[논문]이산화염소수를 활용한 오이 노균병 친환경방제

김병련; 함수상; 권미경; 김윤정; 김운섭; 송정영; 오상근; 주정일

doi:10.5423/rpd.2021.27.4.149

이산화염소수를 활용한 오이 노균병 친환경방제
Environment-Friendly Control of Cucumber Downy Mildew Using Chlorine Dioxide 원문보기

Research in plant disease = 식물병연구, v.27 no.4, 2021년, pp.149 - 154

김병련 (충청남도농업기술원) , 함수상 (충청남도농업기술원) , 권미경 (충청남도농업기술원) , 김윤정 (충청남도농업기술원) , 김운섭 (충청남도농업기술원) , 송정영 (영바이오랩) , 오상근 (충남대학교 응용생물학과) , 주정일 (충청남도농업기술원)

초록
AI-Helper

Pseudoperonospora cubensis는 오이를 포함한 박과작물에 강한 병원성을 나타낸다. 이러한 노균병의 친환경 방제를 위하여 이산화염소수를 적용하였는데, 희석 분무살포하는 경우에 41.2%의 방제효과가 있었지만, 가열연막살포한 경우에는 80.9%의 높은 방제효과를 얻을 수 있었다. 이러한 결과로 노균병과 같이 습도에 큰 영향을 받는 병해의 친환경 방제 시 연막 방제는 기존의 희석살포 방제법보다 더욱 효과적으로 적용 가능한 것을 확인할 수 있었다.

Abstract ▼ AI-Helper

Pseudoperonospora cubensis (downy mildew) is highly virulent to various Cucurbitaceae crops, including cucumber (Cucumis sativus). We tested chlorine dioxide application in a plastic greenhouse for environment-friendly control of downy mildew disease. Spraying diluted chlorine dioxide suppressed downy mildew disease with 41.2% control efficacy. Thermal fogging with chlorine dioxide had a high control efficacy of 80.9%, confirming that this approach is useful for environment-friendly downy mildew control. Using thermal fogging to control diseases that are greatly affected by humidity, such as downy mildew, may be more effective compared with conventional dilution spray control methods.

주제어

표/그림 (4)

그림 Fig. 1. Symptomatic development of downy mildew on cucumber leaves. Each picture represents early (A), mid- (B), and late (C) stage of disease progress of downy mildew. Pictures were obtained 3, 7, and 15 days post-infection for early, mid-, and late disease symptoms, respectively.
표 Table 1. Effect of treatment methods using chlorine dioxide for the control of downy mildew on cucumber in plastic greenhouse
그림 Fig. 2. Suppression of downy mildew disease by the treatment methods of chlorine dioxide in plastic greenhouse test. Pictures for disease symptoms of downy mildew were taken at 28 days post-infection: leaf spraying (A), thermal fogging (B), thermal fogging only with diffusig agent (C), and untreated control (D).
그림 Fig. 3. Changes in humidity (A) and temperature (B) in plastic greenhouses before and after treatment with chlorine dioxide by thermal fogging and leaf spraying. The arrow indicates when chlorine dioxide was treated.

참고문헌 (35)

Benarde, M. A., Israel, B. M., Olivieri, V. P. and Granstrom, M. L. 1965. Efficiency of chlorine dioxide as a bactericide. Appl. Microbiol. 13: 776-780.

상세보기
Chang, T. H., Lim, T. H., Kim, I. Y., Choi, G. J., Kim, J.-C., Kim, H. T. et al. 2000. Effect of phosphorous acid on control of phytophthora blight of red-pepper and tomato, and downy mildew of cucumber in the greenhouse. Korean J. Pestic. Sci. 4: 64-70. (In Korean)
Chen, Z., Zhu, C., Zhang, Y., Niu, D. and Du, J. 2010. Effects of aqueous chlorine dioxide treatment on enzymatic browning and shelf-life of fresh-cut asparagus lettuce (Lactuca sativa L.). Postharvest Biol. Technol. 58: 232-238.

상세보기
Choi, G.-J., Yu, J.-H., Jang, K.-S., Kim, H.-T., Kim, J.-C. and Cho, K.-Y. 2004. In vivo antifungal activities of surfactants against tomato late blight, red pepper blight, and cucumber downy mildew. J. Korean Soc. Appl. Biol. Chem. 47: 339-343. (In Korean)
Choi, S., Beuchat, L. R., Kim, H. and Ryu, J. H. 2016. Viability of sprout seeds as affected by treatment with aqueous chlorine dioxide and dry heat, and reduction of Escherichia coli O157: H7 and Salmonella enterica on pak choi seeds by sequential treatment with chlorine dioxide, drying, and dry heat. Food Microbiol. 54: 127-132.

상세보기
Han, Y., Linton, R. H., Nielsen, S. S. and Nelson, P. E. 2001. Reduction of Listeria monocytogenes on green peppers (Capsicum annuum L.) by gaseous and aqueous chlorine dioxide and water washing and its growth at 7℃. J. Food Prot. 64: 1730-1738.

상세보기
Kim, G. H., Park, J. Y., Cha, J. H., Jeon, C. S., Hong, S. J., Kim, Y. H., et al. 2011. Control effect of major fungal diseases of cucumber by mixing of biofungicides registered for control of powdery mildew with other control agents. Korean J. Pestic. Sci. 15: 323-328. (In Korean)
Kim, M.-H., Kim, Y.-J., Kim, K.-S., Song, Y.-B., Seo, W.-J., and Song, K. B., 2009. Microbial changes in hot peppers, ginger, and carrots treated with aqueous chlorine dioxide or fumaric acid. Korean J. Food Preserv. 16: 1013-1017. (In Korean)
Kim, Y.-K., Ryu, J.-D., Ryu, J.-G., Lee, S. Y. and Shim, H.-S. 2003. Control of downey mildew occurred on cucumber cultivated under plastic film house condition by optimal application of chemical and installation of ventilation fan. Korean J. Pestic. Sci. 7: 223-227. (In Korean)
Kim, Y.-K. Park, S.-H., Um, D.-O., Hong, S.-J., Cho, J.-L., Ahn, N.-H. et al. 2018. Control of cucumber downy mildew using resistant cultivars and organic materials. Res. Plant Dis. 24: 153-161. (In Korean)

원문보기 상세보기
Korean Society of Plant Pathology. 2009. List of Plant Disease in Korea. 5th ed. The Korean Society of Plant Pathology, Suwon, Korea. 853 pp.
Lebeda, A. and Cohen, Y. 2012. Fungicide resistance in Pseudoperonospora cubensis, the causal pathogen of cucurbit downy mildew. In: Fungicide Resistance in Crop Protection: Risk and Management, ed, by T. S. Thind, pp. 44-63. CABI, Wallingford, UK.
Lee, J.-S., Han, K.-S., Lee, S.-C. and Soh, J.-W. 2013a. Screening for resistance to downy mildew among major commercial cucumber varieties. Res. Plant Dis. 19: 188-195. (In Korean)

원문보기 상세보기
Lee, S. Moon, H.-K., Lee, S.-W., Moon, J.-N., Lee, S.-H. and Kim, J.-K. 2013b. Enhanced antimicrobial effectiveness of Omija (Schizamdra chinesis Baillon) by ClO 2 (chlorine dioxide) treatment. Korean J. Food Preserv. 20: 871-876. (In Korean)

원문보기 상세보기
Lee, S. Y., Weon, H. Y., Kim, J. J. and Han, J. H. 2013c. Cultural characteristics and mechanism of Bacillus amyloliquefacien subsp. plantarum CC110 for biological control of cucumber downy mildew. Korean J. Pestic. Sci. 17: 428-434. (In Korean)

원문보기 상세보기
Lee, S. Y., Weon, H. Y., Kim, J. J. and Han, J. H. 2013d. Selection of Bacillus amyloliquefaciens CC110 for biological control of cucumber downy mildew caused by Pseudoperonospora cubensis. Korean J. Mycol. 41: 261-267. (In Korean)

원문보기 상세보기
Mahovic, M. J., Tenney, J. D. and Bartz, J. A. 2007. Applications of chlorine dioxide gas for control of bacterial soft rot in tomatoes. Plant Dis. 91: 1316-1320.

상세보기
Mari, M., Bertolini, P. and Pratella, G. C. 2003. Non-conventional methods for the control of post-harvest pear diseases. J. Appl. Microbiol. 94: 761-766.

상세보기
Meng, R., Han, X. Y., Wu, J., Zhao, J. J., Lu, F. and Wang, W. Q. 2017. Resistance dynamics of Pseudoperonospora cubensis to metalaxyl and azoxystrobin and control efficacy of seven fungicides against cucumber downy mildew in Hebei Province. J. Plant Prot. 44: 849-855.
Ministry of Agriculture, Food and Rural Affairs. 2020. The Numerical Statement of Agriculture. Food and Rural Affairs, Sejong, Korea, pp. 98-99.
National Agricultural Products Quality Management Service. 2021. List of Organic Materials. National Agricultural Products Quality Management Service, Gimcheon, Korea.
National Institute of Agricultural Sciences. 2018. Pesticide Registration Test: Pesticide Efficacy and Crop Safety Guidelines, Fungicide Section. National Institute of Agricultural Sciences, Wanju, Korea, pp. 423-425.
Park, J.-W., Kim, Y.-K., Park, S.-H., Hong, S.-J., Shim, C.-K., Kim, M.-J. et al. 2016. Effect of organic materials and the removal of apical shoot on controlling cucumber downy mildew. Korean J. Org. Agric. 24: 919-929. (In Korean)

원문보기 상세보기
Park, S.-D., Kwon, T. Y., Lim, Y.-S., Jung, K. C. and Choi, B.-S. 1996. Disease survey in melon, watermelon, and cucumber with different successive cropping periods under vinylhouse conditions. Korean J. Plant Pathol. 12: 428-431. (In Korean)
Park, S. S., Sung, J. M., Jeong, J. W., Park, K. J. and Lim, J. H. 2012. Efficacy of electrolyzed water and aqueous chlorine dioxide for reducing pathogenic microorganism on Chinese cabbage. Korean J. Food Sci. Technol. 44: 240-246.

원문보기 상세보기
Roberts, R. G. 1994. Integrating biological control into postharvest disease management strategies. Hortic. Sci. 29: 758-762.
R Core Team. 2017. R foundation for statistical computing ver. 3.4.0. R Foundation for Statistical Computing, Vienna, Austria.
Ryu, S.-H. 2007. Effects of aqueous chlorine dioxide against Escherichia coli O157:H7 and Listeria monocytogenes on broccoli served in foodservice institutions. J. Korean Soc. Food Sci. Nutr. 36: 1622-1627. (In Korean)

원문보기 상세보기
Savory, E. A., Granke, L. L., Quesada-Ocampo, L. M., Varbanova, M., Hausbeck, M. K. and Day, B. 2011. The cucurbit downy mildew pathogen Pseudoperonospora cubensis. Mol. Plant Pathol. 12: 217-226.

상세보기
Sharma, D. R., Gupta, S. K. and Shyam, K. R. 2003. Studies on downy mildew of cucumber caused by Pseudoperonospora cubensis and its management. J. Mycol. Plant Pathol. 33: 246-251.
Sun, S., Lian, S., Feng, S., Dong, X., Wang, C., Li, B. et al. 2017. Effects of temperature and moisture on sporulation and infection by Pseudoperonospora cubensis. Plant Dis. 101: 562-567.

상세보기
Sun, X., Bai, J., Ference, C., Wang, Z., Zhang, Y., Narciso, J. et al. 2014. Antimicrobial activity of controlled-release chlorine dioxide gas on fresh blueberries. J. Food Prot. 77: 1127-1132.

상세보기
Urban, J. and Lebeda, A. 2006. Fungicide resistance in cucurbit downy mildew: methodological, biological and population aspects. Ann. Appl. Biol. 149: 63-75.

상세보기
Yao, K.-S., Hsieh, Y.-H., Chang, Y.-J., Chang, C.-Y., Cheng, T.-C. and Liao, H.-L. 2010. Inactivation effect of chlorine dioxide on phytopathogenic bacteria in irrigation water. J. Environ. Eng. Manage. 20: 157-160.
Zhao, X., Ren, L., Yin, H., Zhou, J., Han, J. and Luo, Y. 2012. Sensitivity of Pseudoperonospora cubensis to dimethomorph, metalaxyl and fosetyl-aluminium in Shanxi of China. Crop Prot. 43: 38-44.

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