[논문]인진호 열수 추출물이 thioacetamide에 의해 유발된 간손상에 미치는 간보호 효과

김민주; 이진아; 신미래; 박해진; 노성수

doi:10.4163/jnh.2021.54.4.412

인진호 열수 추출물이 thioacetamide에 의해 유발된 간손상에 미치는 간보호 효과
Protective effect of Artemisiae Capillaris Herba water extract on liver injury induced by thioacetamide 원문보기

Journal of nutrition and health, v.54 no.4, 2021년, pp.412 - 421

김민주 (대구한의대학교 한의과대학 본초약리학교실) , 이진아 (대구한의대학교 한의과대학 본초약리학교실) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 박해진 (대구한의대학교 DHU 바이오융복합시험센터) , 노성수 (대구한의대학교 한의과대학 본초약리학교실)

초록
AI-Helper

본 연구는 TAA 복강투여로 유발된 간 손상 동물모델에서 인진호 열수 추출물의 간보호 효능을 평가하였으며 다음과 같은 결론을 얻었다. TAA로 인해 줄어드는 체중은 인진호 열수 추출물을 투여한 군에서 유의하게 증가하였으며, 간손상에 의해 증가한 혈중 암모니아 함량과 MPO 활성은 인진호 열수 추출물 투여군에서 유의하게 감소하였다. 간 조직의 western blotting 결과, 인진호 열수 추출물 투여가 산화적 스트레스 관련 인자들의 발현을 유의적으로 감소시키고, 항산화 관련 인자들의 발현을 유의하게 증가시켰으며, MMPs의 발현은 감소시키고 TIMP-1의 발현은 증가시킴을 확인할 수 있었다. 따라서 인진호 열수 추출물은 TAA로 유발된 간손상 동물모델에서 항산화 작용을 통해 산화적 스트레스를 억제하여 간보호 효과를 보이는 것으로 판단된다.

Abstract ▼ AI-Helper

Purpose: Thioacetamide (TAA) produces reactive oxygen species (ROS) in the liver, and the generated ROS induces liver injury through inflammatory reactions. The current study was undertaken to investigate the hepatoprotective effect of Artemisiae Capillaris Herba water extract (AC), imparted via its antioxidant activity, in an animal model of TAA-induced liver injury. Methods: Animal experiments were conducted in 5 groups: normal, control (TAA 200 mg/kg), SM (TAA 200 mg/kg + silymarin 100 mg/kg), ACL (TAA 200 mg/kg + AC 100 mg/kg), ACH (TAA 200 mg/kg + AC 200mg/kg). TAA (intraperitoneal) and treatment compounds (per oral) were administered for 3 days. Serum levels of ammonia concentration and myeloperoxidase (MPO) activity were subsequently measured. Liver tissues were subjected to western blot analysis for measuring the oxidative stress (NADPH oxidase), anti-oxidative activity (Nrf2, heme oxygenase-1 [HO-1], superoxide dismutase [SOD], catalase, and GPx-1/2), tissue inhibitors of metalloproteinase (TIMP)-1, and matrix metalloproteinases (MMPs) protein expressions. Results: Serum ammonia levels and MPO activity were significantly increased in the TAA-induced control group, whereas groups administered AC treatment showed markedly reduced levels. Western blot analysis revealed significantly increased NOX2 and p22phox expressions, (oxidative stress-related factors) in the TAA-induced control group. These levels were determined to be significantly decreased after AC exposure. Moreover, antioxidant-related factors including Nrf2, HO-1, SOD, catalase, and GPx-1/2 were significantly decreased in the control group and increased in the AC treated groups. In addition, MMP expressions were significantly suppressed in the AC treatment group due to increased levels of TIMP-1. Conclusion: Taken together, these data indicate that exposure to AC reduces the oxidative stress by inhibiting the expression of NADPH oxidase (NOX2 and p22phox) through the Nrf2 signaling pathway. We therefore propose the potential of AC for the prevention and treatment of TAA-induced liver injury.

주제어

표/그림 (6)

표 Table 1. Initial and fnal body weight, body weight change
그림 Fig. 1. Ammonia concentration and MPO activity in serum. Ammonia concentration (A) and MPO activity (B). TAA (200 mg/kg of body weight, I.P) and drug treatment compounds (P.O) were administered for 3 days. Groups are represented as below: Normal, normal rats; Control, TAA induce rats; SM, TAA-induced + silymarin 100 mg/kg body weight rats; ACL, TAA-induced + Artemisiae Capillaris Herba water extract 100 mg/kg body weight rats; ACH TAA-induced + Artemisiae Capillaris Herba water extract 200 mg/kg body weight rats. All data are expressed as means ± SD (n = 8 rats per group). MPO, myeloperoxidase. ^###p < 0.001 vs. Normal, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. Control.
그림 Fig. 2. Expressions of NOX2 and p22^phox protein in liver tissue. The protein expression level of NOX2 (A) and p22^phox (B) were detected by western blotting. TAA (200 mg/kg of body weight, I.P) and drug treatment compounds (P.O) were administered for 3 days. Groups are represented as below: Normal, normal rats; Control, TAA induce rats; SM, TAA-induced + silymarin 100 mg/kg body weight rats; ACL, TAA-induced + Artemisiae Capillaris Herba water extract 100 mg/kg body weight rats; ACH TAA-induced + Artemisiae Capillaris Herba water extract 200 mg/kg body weight rats. All data are expressed as means ± SD (n = 8 rats per group). ^##p < 0.01 and ^###p < 0.001 vs. Normal, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. Control.
그림 Fig. 3. Expressions of anti-oxidant protein in liver tissue. The protein expression level of Nrf2 (A), HO-1 (B), SOD (C), Catalase (D), and GPx-1/2 (E) were detected by western blotting. TAA (200 mg/kg of body weight, I.P) and drug treatment compounds (P.O) were administered for 3 days. Groups are represented as below: Normal, normal rats; Control, TAA induce rats; SM, TAA-induced + silymarin 100 mg/kg body weight rats; ACL, TAA-induced + Artemisiae Capillaris Herba water extract 100 mg/kg body weight rats; ACH TAA-induced + Artemisiae Capillaris Herba water extract 200 mg/kg body weight rats. All data are expressed as means ± SD (n = 8 rats per group). SOD, superoxide dismutase. ^###p < 0.001 vs. Normal, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. Control.
그림 Fig. 4. Expressions of TIMP-1 and MMPs protein in liver tissue. The protein expression level of TIMP-1 (A), MMP-1 (B), MMP-2 (C), and MMP-8 (D) were detected by western blotting. TAA (200 mg/kg of body weight, I.P) and drug treatment compounds (P.O) were administered for 3 days. Groups are represented as below: Normal, normal rats; Control, TAA induce rats; SM, TAA-induced + silymarin 100 mg/kg body weight rats; ACL, TAA-induced + Artemisiae Capillaris Herba water extract 100 mg/kg body weight rats; ACH TAA-induced + Artemisiae Capillaris Herba water extract 200 mg/kg body weight rats. All data are expressed as means ± SD (n = 8 rats per group). TIMP, tissue inhibitors of metalloproteinase; MMP, matrix metalloproteinase. ^###p < 0.001 vs. Normal, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. Control.
그림 Fig. 5. Pathological observation (hematoxylin & eosin) in liver tissue. Normal (A), Control (B), SM (C), ACL (D), and ACH (E). TAA (200 mg/kg of body weight, I.P) and drug treatment compounds (P.O) were administered for 3 days. Groups are represented as below: Normal, normal rats; Control, TAA induce rats; SM, TAA-induced + silymarin 100 mg/kg body weight rats; ACL, TAA-induced + Artemisiae Capillaris Herba water extract 100 mg/kg body weight rats; ACH TAA-induced + Artemisiae Capillaris Herba water extract 200 mg/kg body weight rats (×200, scale bar 100 μm).

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