[논문]Thioacetamide로 유발된 간섬유증 동물 모델에서 백작약이 미치는 효능

이세희; 이진아; 신미래; 서부일; 노성수

doi:10.9721/kjfst.2021.53.5.544

Thioacetamide로 유발된 간섬유증 동물 모델에서 백작약이 미치는 효능
Effect of Paeoniae Radix Alba on a thioacetamide induced liver fibrosis mice model 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.53 no.5, 2021년, pp.544 - 552

이세희 (대구한의대학교 한의과대학 본초약리학교실) , 이진아 (대구한의대학교 한의과대학 본초약리학교실) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 서부일 (대구한의대학교 한의과대학 본초약리학교실) , 노성수 (대구한의대학교 한의과대학 본초약리학교실)

초록
AI-Helper

본 연구에서 in vitro를 통해 백작약 열수 추출물의 total polyphenol, total flavonoid 함량, DPPH 및 ABTS radical 소거능 측정으로 높은 항산화능을 확인했으며, 백작약 열수 추출물 투여로 인한 AST, ALT, ammonia 및 MPO 수치의 호전을 확인하였다. 또한 간 조직을 이용한 western blot 실시 후 모든 인자에서 유의적인 차이를 확인하였다. 이 결과를 토대로, 백작약 열수 추출물이 TAA로 인한 산화적 스트레스를 Nrf2/Keap1 경로의 활성화를 통해 SIRT1/AMPK/NF-kBp65를 조절하였으며, 섬유화 관련 단백질의 발현을 억제함으로써 간섬유증에 대한 백작약 열수 추출물의 긍정적인 효과가 있음을 제시하였다. 따라서 선행 연구를 통해 TAA로 유발한 급성 간손상 동물 모델에서 간보호 효과를 나타낸 백작약 열수 추출물이 TAA로 유발한 간섬유증 동물 모델에서 산화적 스트레스 억제와 간 기능 개선을 나타냈으며 향후 후보 소재로서 가능성이 있다고 판단된다.

Abstract ▼ AI-Helper

This study investigated the anti-fibrotic and antioxidant effects of Paeonia Radix Alba water extract (PR) on thioacetamide (TAA)-induced liver fibrosis in a mouse model and its underlying mechanisms. Liver fibrosis was induced by intraperitoneal injection of TAA (three times a week) for 8 weeks. Furthermore, silymarin (50 mg/kg body weight) and PR (200 mg/kg body weight) were administered for 8 weeks. PR treatment downregulated aspartate aminotransferase (AST), alanine aminotransferase (ALT), ammonia, and myeloperoxidase levels. Moreover, PR treatment downregulated NOX2 and p47phox and upregulated antioxidant enzymes by activating the Nrf2/Keap1 signaling pathway. Furthermore, PR inhibited the factors associated with fibrosis, such as α-SMA and collagen I. AMPK/SIRT1 was upregulated by PR treatment. Overall, these results suggest that PR attenuates liver fibrosis by regulating the Nrf2/Keap1 and AMPK/SIRT1/NF-κB signaling pathways through the inhibition of oxidative stress. Hence, PR has potential as a remedy for preventing and treating liver fibrosis.

주제어

표/그림 (8)

표 Table 1. Total polyphenol and total flavonoid contents of Paeoniae Radix Alba water extract
표 Table 2. Scavenging activity (IC50) of PR on DPPH and ABTS free radical
그림 Fig. 1. Levels of AST, ALT, ammonia and MPO in serum. Serum AST level (A), Serum ALT level (B), Serum ammonia concentration (C), Serum MPO concentration (D). Normal, normal mice; Control, TAA-induced control mice; Silymarin, TAA-induced with silymarin 50 mg/kg body weight/day mice; PR, TAA-induced with Paeoniae Radix water extract 200 mg/kg body weight/day mice. All data are expressed as means±SD (n=9 mice per group). Significance: ###p<0.001 vs. Normal, *p<0.05, **p<0.01, and ***p<0.001 vs. Control.
그림 Fig. 2. Expressions of NADPH oxidase proteins in liver. Normal, normal mice; Control, TAA-induced control mice; Silymarin, TAA-induced with silymarin 50 mg/kg body weight/day mice; PR, TAA-induced with Paeoniae Radix water extract 200 mg/kg body weight/day mice. All data are expressed as means±SD (n=9 mice per group). Significance: ##p<0.01, ###p<0.001 vs. Normal, *p<0.05, **p<0.01 vs. Control.
그림 Fig. 3. Expressions of antioxidant-related proteins in liver. Normal, normal mice; Control, TAA-induced control mice; Silymarin, TAAinduced with silymarin 50 mg/kg body weight/day mice; PR, TAA-induced with Paeoniae Radix water extract 200 mg/kg body weight/day mice. All data are expressed as means±SD (n=9 mice per group). Significance: #p<0.05, ##p<0.01 vs. Normal, *p<0.05, **p<0.01 vs. Control.
그림 Fig. 4. Expressions of activated proteins and inflammatory proteins in liver. Normal, normal mice; Control, TAA-induced control mice; Silymarin, TAA-induced with silymarin 50 mg/kg body weight/day mice; PR, TAA-induced with Paeoniae Radix water extract 200 mg/kg body weight/day mice. All data are expressed as means±SD (n=9 mice per group). Significance: #p<0.05, ##p<0.01, and ###p<0.001 vs. Normal, *p<0.05, **p<0.01 vs. Control.
그림 Fig. 5. Expressions of α-SMA and Collagen I in liver. Normal, normal mice; Control, TAA-induced control mice; Silymarin, TAA-induced with silymarin 50 mg/kg body weight/day mice; PR, TAA-induced with Paeoniae Radix water extract 200 mg/kg body weight/day mice. All data are expressed as means±SD (n=9 mice per group). Significance: ###p<0.001 vs. Normal, *p<0.05, **p<0.01, and ***p<0.01 vs. Control.
그림 Fig. 6. Histopathological analysis. A, hematoxylin and eosin (H&E) staining; B, Masson’s trichrome (MT) staining. (Original magnification ×200, magnification bar=100 μm). Normal, normal mice; Control, TAA-induced control mice; Silymarin, TAA-induced with silymarin 50 mg/ kg body weight/day mice; PR, TAA-induced with Paeoniae Radix water extract 200 mg/kg body weight/day mice.

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