[논문]상황버섯 가수분해물의 피부 항노화 효능에 대한 연구

김태준; 곽병문; 김희택

doi:10.6114/jkood.2021.34.2.001

상황버섯 가수분해물의 피부 항노화 효능에 대한 연구
Studies on Skin Anti-aging Efficacy of Hydrolysate from Phellinus igniarius 원문보기

韓方眼耳鼻咽喉皮膚科學會誌 = The journal of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, v.34 no.2, 2021년, pp.1 - 20

김태준 (세명대학교 한의과대학 한방안이비인후피부과) , 곽병문 (세명대학교 보건바이오대학 화장품뷰티생명공학부) , 김희택 (세명대학교 한의과대학 한방안이비인후피부과)

Abstract ▼ AI-Helper

Objective : The object of this study was to assess the efficacy of hydrolysate from Phellinus igniarius(HPI) on anti-aging activities in vitro measurement and mini clinical study performed on 5 subjects. Methods : To evaluate skin anti-aging efficacy of HPI, 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity, type I collagen synthesis, inhibition of nitric oxide(NO) production, inhibiton of tyrosinase, hyaluronan synthase(HAS)2, 3 mRNA expression were measured in vitro. Also, mini clinical study of skin hydration was performed on 5 subjects using HPI in distilled water(DW) and 1,3-butylene glycol diluted solution(30% in DW). Results : 1. DPPH radical scavenging activity of HPI was increased in a dose-dependant. 2. Type I collagen synthesis was increased in 50, 100 and 500㎍/㎖ of HPI. 3. NO production was not inhibited in all concentrations of HPI. 4. Tyrosinase was inhibited in 500, 1000, 2500 and 5000㎍/㎖ of HPI. 5. HAS2 mRNA expression was increased in 50, 100, 150 and 200㎍/㎖ of HPI, HAS3 mRNA expression was increased in 100, 150, and 200㎍/㎖ of HPI. 6. In the mini clinical study of 5 subjects, there was a difference in skin hydration over time for each solutions, but it was not statistically siginificant. Conclusions : HPI increased DPPH radical scavenging activity, type I collagen synthesis, and HAS2, HAS3 mRNA expression. HPI also suppressed tyrosinase. The findings of this study suggest that HPI can be used as an skin anti-aging material.

주제어

표/그림 (25)

그림 Fig. 1. Process Flow Diagram for Supercritical Extraction from Phellinus igniarius (Kwak BM, 2019)
표 Table 1. Condition of Supercritical Extraction from Phellinus igniarius
표 Table 2. Realtime PCR Primer Sequences
그림 Fig. 2. Accumulation of Extracted Protein Yield from Phellinus igniarius. Yield of protein from Phellinus igniarius depends on the extraction times. First time yield of protein is about 4.5%. Second time yield of protein is about 5.6%. Third time yield of protein is about 5.8%.
그림 Fig. 3. Protein from Phellinus igniarius Light Brown Protein Products
그림 Fig. 4. Chromatography of Hydrolysate by Papain Over Time on ESI-MS. ESI-mass spectrometer focused on 200-2000m/z, amu. Green indicates 24hrs, Red indicates 48hrs, Blue indicates 72hrs. As time passed, the intensity of high molecular compound decreased.
그림 Fig. 5. Chromatography of Hydrolysate by Bromelain Over Time on ESI-MS. ESI-mass spectrometer focused on 200-2000m/z, amu. Red indicates 72hrs, Blue indicates 84hrs. During the last 12hrs, the intensity of high molecular compound decreased.
그림 Fig. 6. Chromatography of Hydrolysate by Enzyme Mixture Over Time on ESI-MS. ESI-mass spectrometer focused on 200-2000m/z, amu. Green indicates 24hrs, Red indicates 48hrs, Blue indicates 72hrs. As time passed, the intensity of high molecular compound decreased.
그림 Fig. 7. Chromatography of Comparison. ESI-mass spectrometer focused on 70-200m/z, amu(A), and 250-2000m/z, amu(B). Red indicates papain for 72hrs, green indicates bromelain for 84hrs, blue indicates mixture for 72hrs. Mixture has the best hydrolysis efficiency.
그림 Fig. 8. Hydrolysate from Phellinus igniarius by Enzyme Mixture Dark Black Hydrolysate from Phellinus igniarius Was Made by Enzyme Mixture for 72 Hours.
그림 Fig. 9. Effect of Hydrolysate from Phellinus igniarius on DPPH Radical Scavenging Test. The concentrations of sample were 1, 5, 10, 50, 100, 500 and 1000㎍/㎖ of hydrolysate from Phellinus igniarius. Vit C was used as positive control. Activities were determined by measurement of absorbance at 517㎚. ^* p<0.05 vs 1㎍/㎖. The data was obtained from 3 independent experiments. HPI: Hydrolysate from Phellinus igniarius. ; Vit C: Vitamin C.
그림 Fig. 10. Effect of Hydrolysate from Phellinus igniarius on Cell Viability. CCD-986sk cells were treated with concentrations of sample 5, 10, 50, 100, 500 and 1000㎍/㎖ of hydrolysate from Phellinus igniarius. Cytotoxicity was measured using an MTT assay. Cell viability are presented as a percentage of the control group. HPI: Hydrolysate from Phellinus igniarius.
그림 Fig. 11. Expression of Type Ⅰ Collagen on CCD-986sk Cells by Hydrolysate from Phellinus igniarius. The concentrations of sample were 5, 10, 100 and 500 ㎍/㎖ of hydrolysate from Phellinus igniarius. Incubated time of CCD-986sk cells was 24hrs. ^* p<0.05, ^{* *} p<0.01 vs control. The data was obtained from 3 independent experiments. HPI: Hydrolysate from Phellinus igniarius.
그림 Fig. 12. Effect of Hydrolysate from Phellinus igniarius on Cell Viability. RAW 264.7 cells were treated with concentrations of sample 5, 10, 50, 100, 500 and 1000㎍/㎖ of hydrolysate from Phellinus igniarius. Cytotoxicity was measured using an MTT assay. Cell viability are presented as a percentage of the control group. HPI: Hydrolysate from Phellinus igniarius.
그림 Fig. 13. Inhibition of Nitric Oxide Production on RAW 264.7 Cells by Hydrolysate from Phellinus igniarius. NO production was measured by the griess reaction assay. The concentrations of sample were 5, 10, 50, 100 and 500㎍/㎖ of hydrolysate from Phellinus igniarius. Incubated time of RAW 264.7 cell was 24hrs. HPI: Hydrolysate from Phellinus igniarius.
그림 Fig. 14. Inhibition of Tyrosinase by Hydrolysate from Phellinus igniarius. The concentrations of sample were 250, 500, 1000, 2500 and 5000㎍/㎖ of hydrolysate from Phellinus igniarius. Arbutin was used as positive control. Activities were determined by measurement of absorbance at 490㎚. ^* p<0.05, ^{* *} p<0.01 vs 250㎍/㎖. The data was obtained from 3 independent experiments. HPI: Hydrolysate from Phellinus igniarius.
그림 Fig. 15. Effect of Hydrolysate from Phellinus igniarius on HAS2 mRNA Expression in HaCat Cells. HaCat cells were treated with concentrations of sample 10, 50, 100, 150 and 200㎍/㎖ of hydrolysate from Phellinus igniarius for 24hrs. HAS2 mRNA expression was measured by real time PCR. ^{* *} p<0.01, ^{* * *} p<0.001 vs control. The data was obtained from 3 independent experiments. HPI: Hydrolysate from Phellinus igniarius.
그림 Fig. 16. Effect of Hydrolysate from Phellinus igniarius on HAS3 mRNA Expression in HaCat Cells. HaCat cells were treated with concentrations of sample 10, 50, 100, 150 and 200㎍/㎖ of hydrolysate from Phellinus igniarius for 24hrs. HAS3 mRNA expression was measured by real time PCR. ^{* *} p<0.01, ^{* * *} p<0.001 vs control. The data was obtained from 3 independent experiments. HPI: Hydrolysate from Phellinus igniarius.
그림 Fig. 17. Moisturizing Ability on Skin of Subject 1 by Hydrolysate from Phellinus igniarius. A: Distilled water, B: Hydrolysate 1000㎍/㎖ in distilled water, C: 1,3-butylene glycol, D: Hydrolysate 1000㎍/㎖ in 1,3-butylene glycol.
그림 Fig. 18. Moisturizing Ability on Skin of Subject 2 by Hydrolysate from Phellinus igniarius. A: Distilled water, B: Hydrolysate 1000㎍/㎖ in distilled water, C: 1,3-butylene glycol, D: Hydrolysate 1000㎍/㎖ in 1,3-butylene glycol.
표 Table 3. Inter-group Comparison Test Statistics (A Versus B)
표 Table 4. Inter-group Comparison Test Statistics (C Versus D)
그림 Fig. 19. Moisturizing Ability on Skin of Subject 3 by Hydrolysate from Phellinus igniarius. A: Distilled water, B: Hydrolysate 1000㎍/㎖ in distilled water, C: 1,3-butylene glycol, D: Hydrolysate 1000㎍/㎖ in 1,3-butylene glycol.
그림 Fig. 20. Moisturizing Ability on Skin of Subject 4 by Hydrolysate from Phellinus igniarius. A: Distilled water, B: Hydrolysate 1000㎍/㎖ in distilled water, C: 1,3-butylene glycol, D: Hydrolysate 1000㎍/㎖ in 1,3-butylene glycol.
그림 Fig. 21. Moisturizing Ability on Skin of Subject 5 by Hydrolysate from Phellinus igniarius. A: Distilled water, B: Hydrolysate 1000㎍/㎖ in distilled water, C: 1,3-butylene glycol, D: Hydrolysate 1000㎍/㎖ in 1,3-butylene glycol.

참고문헌 (60)

Kim ID, Kwon RH, Heo YY, Jung HJ, Kang HY, Ha BJ. Supercritical Extraction of Oriental Herb : Anti-aging and Anti-wrinkle Effects. Korean J. Biotechnol. Bioeng. 2008;23(6):529-34.
Nam HJ, Kim YB. Aging and skin aging. J Korean Med Ophthalmol Otolaryngol Dermatol. 2004;17(1):16-33.
Song H, Kim MS. Anti-aging & Skin Hydration Effects of Spore oil Extracted from Ganoderma lucidum. Journal of Convergence for Information Technology. 2020;10(5):232-8.

원문보기 상세보기
Kim SH, Jang HJ. Effect of the Cosmetics Products Containing Morinda citrifolia on the 30-50 Middle-aged Women's Skin. The Korean Beauty Management Society. 2016;4(1):1-7.
Park HS, Kim YM, Kim HT. A Clinical Study for the Efficacy and Safety of Functional Cosmetics Containing Humulus japonicus Extract in Patients with Dry Skin due to Mild Atopic Dermatitis. J Korean Med Ophthalmol Otolaryngol Dermatol. 2019;32(2):24-58.

원문보기 상세보기
Soh SY, Chun YJ. Cosmeceutical Properties of Extracts of Torreya nucifera and Alpinia henryi and Formulation Characteristics of Mask Pack Containing Extracts of These. Journal of the Korea Academia-Industrial cooperation Society. 2020;21(8):36-43.

원문보기 상세보기
Kim GY, Jung HW, Jung DJ, Song HB, Lee HG. Effects of Shiitake Mushroom on Antiplatelet Aggregation and Anti-thrombotic. J. Oriental Physiology & Pathology. 2013;27(2):239-45.
Seo MS, Jang YA, Lee JT. The Study of Cosmeceutical Activities from Lentinula edodes extracts and Application a Natural Cosmetic Material. Journal of Oil & Applied Science. 2018;35(4):1003-12.

원문보기 상세보기
Kim AR, Kim JE, Park SN. Antioxidative Activity and Component Analysis of Phellinus linteus Extracts. J. Soc. Cosmet. Sci. Korea. 2011;37(4):309-18.

원문보기 상세보기
Lee SJ. Dohae Bonchogangmok. Seoul: Komoonsa. 1975:500-5.
Pyo MY, Hyun SM, Yang KS. Effects of phellinus linteus extracts on the humoral immune response in normal and cyclophosphamide-treated mice. The Journal of Applied Pharmacology. 2001;9(3):194-200.
Rhee YK, Han MJ, Park SY, Kim DH. In vitro and in vivo Antitumor Activity of the Fruit Body of Phellinus linteus. Korean J. Food Sci. Technol. 2000;32(2):477-80.
Lee YH. Effect of Phellinus Linteus Grown in Germinated Brown Rice on Atopic Dermatitis. J. Kor. Soc. Cosm. 2007;13(2):514-9.
Song CH, Ra KS, Yang BK, Jeon YJ. Immuno-stimulating activity of phellinus linteus. The Korean Journal of Mycology. 1998;26(1):86-90.
Seo JH. The effects of phellinus linteus extract administration on aerobic exercise ability and blood lactate concentration the during the supramaximal progressive loaded exercise by athletes. The Korean Journal of Physical Education. 2008;47(2):499-505.
Jung ME, Ham SS, Nam SM, Kang IJ, Kim SJ, Chung CK. Biochemical and Histological Effects of Phellinus linteus Methanol Extract on Liver Lipid Metabolism of Rats Fed CCl4 and High Fat. J. Korean Soc. Food Sci. Nutr. 2001;30(2):331-7.
Jeoung YJ, Choi SY, An CS, Jeon YH, Park DK, Lim BO. Comparative Effect on Anti-Inflammatory Activity of the Phellinus linteus and Phellinus linteus Grown in Germinated Brown Rice Extracts in Murine Macrophage RAW 264.7 Cells. Korean J. Medicinal Crop Sci. 2009;17(2):97-101.
Ji JH, Kim MN, Chung CK, Ham SS. Antimutagenic and Cytotoxicity Effects of Phellinus linteus Extracts. J. Korean Soc. Food Sci. Nutr. 2000;29(2):322-8.
Yun WS, Jung HA, Roh SS. Effect of Phellinus igniarius Quel Extract on the Anti-inflammatory, Anti allergy, Antioxidant, Anti-wrinkle. J Korean Med Ophthalmol Otolaryngol Dermatol. 2010;23(1):75-93.
Kwak BM, Kim EH, Kim YM, Kim HT. Component analysis of four-part extracts from Chamaecyparis obtusa Endl. by supercritical fluid extraction anti-inflammatory effect on RAW 264.7cells. Journal of Exercise Rehabilitation. 2019;15(5):723-30.

상세보기
Han LL. Studies on skin moisturizing and anti-inflammatory efficacy of peptide and amino acid hydrolysate from ginseng seed. [master's thesis] Suwon:Ajou University. 2017:1-66.
KDA Textbook Editing Board. Dermatology 6th edition. Seoul:Medbook. 2014:2-51.
Kim JE, Choi YS, Kim HK, Jang YA. Collagen synthesis ability and inhibitory effect of MMPs in keratinocytes of Lysimachia christinae Hance Extract. Journal of the Korean Applied Science and Technology. 2020;37(4):820-9.

원문보기 상세보기
Kim TJ, Kim YM, Kim HT. Effects of Naetakcheongeum-san on Anti-inflammatory Activities in RAW 264.7 cells. J Korean Med Ophthalmol Otolaryngol Dermatol. 2018;31(1):12-21.

원문보기 상세보기
Chen CY, Kuo PL, Chen YH, Huang JC, Ho ML, Lin RJ, et al. Tyrosinase inhibition free radical scavenging antimicroorganism and anticancer proliferation activities of sapindus mukorossi extracts. Journal of the Taiwan Institute of Chemical Engineers. 2010;41(2):129-35.

상세보기
Triggs-Raine B, Natowicz MR. Biology of hyaluronan: Insights from genetic disorders of hyaluronan metabolism. World J Biol Chem. 2015;6(3):110-20.

상세보기
Kim HS, Ko KS. Antioxidant and Anti-inflammatory Effects of Ginseng Berry Ethanol Extracts as a Cosmetic Ingredient. Asian J Beauty Cosmetol. 2020;18(3):389-97.

상세보기
Yoon JH, Park JH, Kim BR. Skin Whitening Effect of Sedum kamtschaticum Fisch. Solvent Fractions. Journal of the Korean Applied Science and Technology. 2020;37(5):1239-47.

원문보기 상세보기
Shim JH. Anti-aging Effect of Sulfuretin in UVA-irradiated Normal Human Epidermal Keratinocytes. Asian J Beauty Cosmetol. 2020;18(3):265-72.

상세보기
Sohn EH, Roh HS, Par YS, Sohn ES, Kang SC, Kang NS, et al. Phellinus Iinteus; Market and Technology Trends Analysis. Korean J. Biotechnol. Bioeng. 2008;23(2):109-17.
Kang MY, Kim S, Yun HJ, Nam SH. Antioxidative activity of the extracts from browned oak mushroom(Lentinula edodes) with unmarketable quality. Korean J. Food Sci. Technol. 2004;36(4):648-54.
Kim MS, Park TJ, Lim JS, Kim SY. Effect of Agaricus biorenovate Extract on Collagen Synthesis and Matrix Metalloproteinase-1 Production in Human Dermal Fibroblast. Korean Society for Biotechnology and Bioengineering Journal. 2019;34(1):49-53.

상세보기
Kim JE, Lee SY, Chang YH, Jim MH. Effect of Glucosylceramides and Sterols Isolated from Agaricus Blazei Extract on Improvement of Skin Cell. J. Soc. Cosmet. Sci. Korea. 2020;46(2):105-17.

원문보기 상세보기
Jang HL, Park SY, Nam JS. Effect of Extraction Solvent on the Antioxidant Activity of Lentinula edodes GNA01 Extract. Korean J. Food Nutr. 2017;30(1):51-8.

원문보기 상세보기
Hong WK, Shin JH, Lee YH, Park DK, Choi GS. The Clinical Effect of Phellinus linteus Grown on Germinated Brown Rice in the Treatment of Atopic Dermatitis. Kor. J. Herbology. 2008;23(1):103-8.
Shon MY, Seo KI, Choi SY, Sung NJ, Lee SW, Park SK. Chemical Compounds and Biological Activity of Phellinus baumii. J. Korean Soc Food Sci Nutr. 2006;35(5):524-9.

원문보기 상세보기
Choi CH. Saengtaeboncho. Paju:Fishwood. 2018:366-80.
Dong-A Editing Board. Dong-A Chinese character Korean Dictionary 5th edition. Seoul:Dong-A Publishing. 1989:1443-4.
Choo J. Bongyeongsojeung. Seoul:Medibook. 2001:657-8.
Ko BS. Sinpyeonjungyagdaesajeon. Taipei: Sinmunpung Publisher. 1982:1479-80.
Heo J. Donguibogam. Hadong:Donguibogam Publisher. 2006:2139-40.
Ko WS, Kim KJ, Kim NK, Kim YB, Kim JH, Kim HT, et al. Text of Traditional Korean Dermatology & Surgery. Busan:Seonu. 2007:27-142.
Kwak BM. Studies on the anti-inflammatory effect of the supercritical fluid extraction from Chamaecyparis obtusa Endl. [doctor's thesis] Suwon:Ajou University. 2019:1-106.
Rachmawati D, Hutabarat J, Samidjan I, Windarto S. Utilization of papain as feed additive in the fish feed on activity of digestive enzymes, contents of nutrient and minerals of Sangkuriang catfish. AACL Bioflux. 2020;13(5):2738-44.
Choi C, Son GM, Cho YJ, Chun SS, Lim SI, Seok YR. Purificaton and characteristics of bromelain from korean pineapple. J. Korean Agric. Chem. Soc. 1992;35(1):23-9.
Kim SJ, Kim TJ, Kim EH, Kim YM. Anti-inflammatory and Anti-oxidant Studies of Osung-tang Extracts in LPS-Induced RAW 264.7 Cells. J Korean Med Ophthalmol Otolaryngol Dermatol. 2020;33(1):1-11.

원문보기 상세보기
Choi KH, Seo HS. The Experimental Study of Jeondo-san on Antioxidant Effects. Journal of Pharmacopuncture. 2008;11(1): 143-7.

원문보기 상세보기
Yang B, Ji C, Kang J, Chen W, Bi Z, Wan Y. Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts. Int J Mol Med. 2009;23(4):555-60.
Tsuji-Naito K. Ishikura S, Akagawa M, Saeki H. α- Lipoic acid induces collagen biosynthesis involving prolyl hydroxylase expression via activation of TGF-β-Smad signaling in human dermal fibroblasts. Connect Tissue Res. 2010;51(5):378-87.

상세보기
Huang C, Ma WY, Dawson MI, Rincon M, Flavell RA, Dong Z. Blocking activator protein-1 activity, but not activating retinoic acid response element, is required for the antitumor promotion effect of retinoic acid. Proc Natl Acad Sci U S A. 1997;94(11):5826-30.

상세보기
Jeon SH, Seol GH, Suh SH, Park SH. Nitric Oxide-Induced Intracellular Ca2+ Modulation in Macrovascular Endothelial Cells. Korean Circulation J. 2004;34(6):600-9.

상세보기
Lee HJ, Kim JS, Jin CB, Ryu JH. Inhibitory activity of medicinal herbs on nitric oxide synthesis in activated macrophages. Natural Product Sciences. 2005;11(1):16-21.

원문보기 상세보기
Lee TH, Kwak HB, Kim HH, Lee ZH, Chung DK, Baek NI, et al. Methanol extracts of Stewartia koreana inhibit Cyclooxygenase-2 (COX-2) and Inducible Nitric Oxide Synthase (iNOS) Gene Expression by blocking NF-kappa B transactivation in LPS-activated RAW 264.7 cells. Mol. Cells. 2007;23(3):398-404.
Kim CH, Jung HA, Roh SS, Hong SH. Effects of Aloe and Violae herba Extract on the Anti-oxidant, Anti-inflammatory, Anti-wrinkle and Whitening. J Korean Med Ophthalmol Otolaryngol Dermatol. 2010;23(1):23-43.
Mo JH, Oh SJ. Tyrosinase Inhibitory Activity and Melanin Production Inhibitory Activity of the Methanol Extract and Fractions from Dendropanax morbifera Lev. Kor. J. Aesthet. Cosmetol. 2013;11(2):275-80.
Zolghadri S, Bahrami AR, Hassan Khan MT, Munoz-munoz J, Garcia-Molina F, Garcia-Canovas F, et al. A comprehensive review on tyrosinase inhibitors. J Enzyme Inhib Med Chem. 2019;34(1):279-309.

상세보기
Park HS, Ha HY, Kim HT. An Experimental Study on the Effect of Angelica gigas Ethanol Extract on Hyaluronic Acid Synthesis. J Korean Med Ophthalmol Otolaryngol Dermatol. 2018;31(1):32-41.

원문보기 상세보기
Ahn SY, Gil SY, Kwon OS, Chang YH, Jin MH. Skin Hydration Effect of Brasenia schreberi Mucilage Polysaccharide Extract. J. Soc. Cosmet. Sci. Korea. 2017;43(3):223-30.

원문보기 상세보기
Kligman AM, Dogadkina D, Lavker RM. Effects of topical tretinoin on non-sun-exposed protected skin of the elderly. J AM Acad Dermatol. 1993;29(1):25-33.

상세보기
So BH. Device evaluation of skin effect. The Journal of Skin Barrier Research. 2006;8(1):68-75.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증