[논문]해송자 오일의 피부 항노화 및 주름 개선 효과

김형묵; 김태준; 임동빈; 하순봉; 김이화; 차병선; 허효진; 소피아브리토; 이용문; 빈범호; 곽병문

doi:10.6114/jkood.2021.34.4.001

해송자 오일의 피부 항노화 및 주름 개선 효과
Skin Anti-aging and Anti-wrinkle Effects of Pinus koreaiensis Seed Oil 원문보기

韓方眼耳鼻咽喉皮膚科學會誌 = The journal of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, v.34 no.4, 2021년, pp.1 - 11

김형묵 (충북대학교 약학부 응용약학과) , 김태준 (세명대학교 한의과대학 한방안이비인후피부과) , 임동빈 (세명대학교 한의과대학 한의학과) , 하순봉 (세명대학교 한의과대학 한의학과) , 김이화 (세명대학교 한의과대학 한의학과) , 차병선 (아주대학교 자연과학대학 생명과학과) , 허효진 (아주대학교 일반대학원 응용생명공학과) , 소피아브리토 (아주대학교 일반대학원 응용생명공학과) , 이용문 (충북대학교 약학부 응용약학과) , 빈범호 (아주대학교 자연과학대학 생명과학과) , 곽병문 (세명대학교 화장품뷰티생명공학부)

Abstract ▼ AI-Helper

Objectives : The aim of this study was to investigated the anti-aging and anti-wrinkles effects of the pinus koreaiensis seed(PKS) oil. Methods : The anti-oxidant effect was performed by beta-carotene bleaching assay and the intracellular proteome was analyzed expression of each 15 proteins by 2-D electrophoresis. And fatty acid was analysed by gas chromatography. Anti-wrinkle effect was analyzing human skin by the PRIMOS system. Results : Fatty acid analysis of PKS oil has shown oleic acid was 49.7% and linoleic acid was 34.1%. And the antioxidant effect was about 125% compared with alpha-tocoperol(0.1%) by beta carotene bleaching assay. In 2D PAGE analysis, fifteen protein changes in five mechanisms which was collagen synthesis pathway, MMPs, ECM-cell interaction, cytokine, antioxidant enzymes were analyzed. In case of anti-wrinkle effect was proved in vivo by analyzing human skin by the PRIMOS system. The analysis results of eye wrinkles for 4 weeks showed an improvement effect of over 6%. Conclusions : In this study, the amount of protein change in the five mechanism through the cell experiment and the skin anti wrinkle efficacy by the human in vivo test were investigated. As a result pinus koreaiensis seed oil by supercritical extraction could be used as a anti-aging and anti-wrinkle substance for the skin.

주제어

표/그림 (10)

그림 Fig. 1. The Process Flow Diagram for the Supercritical Extraction from Pinus koreaiensis Seed The conditions used for the extraction from PKS were pressure of 350 bar, temperatures of 60 ℃, flow of 140 ㎖/min, particle size of 800-1000㎛.
표 Table 2. Fatty Acid Standard for GC Analysis.
그림 Fig. 2. The Kinetic Graph of Extraction Rate from PKS Supercritical CO₂ flow was 145 ㎖/min, extraction time was 170min, temperature was at 60℃, pressure was 350 bar.
그림 Fig. 3. Gas Chromatography of Fatty Acid Standard and PKS Oil. Chromatogram of PKS oil. GC conditions : 100℃ to 240℃ at 3℃/min., FID : 250℃, (A) Fatty acid standard, (B) Fatty acid from PKS oil
그림 Fig. 4. Effect of Antioxidant Activity by PKS Oil. The concentrations of sample were 1, 10 and 100% of PKS. Alpha-tocoperol(0.1%) was used as a control. The data was obtained from 3 independent experiments.
표 Table 2. Fatty Acid Composition of PKS Oil
그림 Fig. 5. 2D-PAGE Gel Control(A), PKS oil(B). Gel image of proteins extracted from NF cell treated with 500 ppm of PKS oil
표 Table 3. Effect of PKS Oil to Expression of Proteins
표 Table 4. Analysis for Anti-wrinkle Effects
표 Table 5. Analysis for Anti-wrinkle Effects by Pictures

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