[논문]Antibacterial Effect of Eucalyptus Oil, Tea Tree Oil, Grapefruit Seed Extract, Potassium Sorbate, and Lactic Acid for the development of Feminine Cleansers

Yuk, Young Sam

doi:10.7236/ijibc.2021.13.2.82

Antibacterial Effect of Eucalyptus Oil, Tea Tree Oil, Grapefruit Seed Extract, Potassium Sorbate, and Lactic Acid for the development of Feminine Cleansers 원문보기

International journal of internet, broadcasting and communication : IJIBC, v.13 no.2, 2021년, pp.82 - 92

Yuk, Young Sam (Department of Clinical Medical Science, Graduate School of Health and Welfare, Dankook University)

Abstract ▼ AI-Helper

Purpose: It has been reported that the diversity and abundance of microbes in the vagina decrease due to the use of antimicrobial agents, and the high recurrence rate of female vaginitis due to this suggests that a new treatment is needed. Methods: In the experiment, we detected that 10% potassium sorbate solution, 1% eucalyptus oil solution, 1% tea tree oil solution, 400 µL/10 mL grapefruit seed extract solution, 100% lactic acid, 10% acetic acid solution, and 10% lactic acid solution were prepared and used. After adjusting the pH to 4, 5, and 6 with lactic acid and acetic acid in the mixed culture medium, each bacterium was inoculated into the medium and incubated for 72 h at 35℃. Incubate and 0 h each. 24 h. 48 h. The number of bacteria was measured after 72 h. Results: In the mixed culture test between lactic acid bacteria and pathogenic microorganisms, lactic acid bacteria showed good results at pH 5-5.5. Potassium sorbate, which has varying antibacterial activity based on the pH, killed pathogenic bacteria and allowed lactic acid bacteria to survive at pH 5.5. Conclusion: The formulation ratio obtained through this study could be used for the development of a feminine cleanser that can be used as a substitute for antibacterial agents. Further, the findings of this study may be able to solve the problem of antimicrobial resistance in the future.

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표/그림 (9)

표 Table 1. Strains and media used in this study
표 Table 3. The blending ratio of grapefruit seed extract, eucalyptus oil, and tea tree oil that satisfies the conditions for inhibiting pathogenic microorganisms and promoting the growth of lactic acid bacteria, which is a required for vaginal cleansers (pH 5.5)
표 Table 4. The blending ratio of potassium sorbate, grapefruit seed extract, eucalyptus oil, and tea tree oil that inhibits pathogenic microorganisms and allows lactic acid bacteria to grow (pH 5.5)
그림 Figure 1. Growth of lactic acid bacteria in mixed culture of C. albicans and lactic acid bacteria at the appropriate pH and in the presence of lactic acid and acetic acid
그림 Figure 2. Growth of Candida albicans in mixed culture of C. albicans and lactic acid bacteria at the appropriate pH in the presence of lactic acid and acetic acid
표 Table 2. Growth of Candida albicans, Escherichia coli, and Lactobacillus plantarum according to changes in pH and potassium sorbate concentration
표 Table 3. The blending ratio of grapefruit seed extract, eucalyptus oil, and tea tree oil that satisfies the conditions for inhibiting pathogenic microorganisms and promoting the growth of lactic acid bacteria, which is a required for vaginal cleansers (pH 5.5)
표 Table 4. The blending ratio of potassium sorbate, grapefruit seed extract, eucalyptus oil, and tea tree oil that inhibits pathogenic microorganisms and allows lactic acid bacteria to grow (pH 5.5)
그림 Figure 3. Comparison of microbial growth in the presence of a potassium sorbate (10%; 0.2 mL/200 mL), grapefruit seed extract (400 μL/10 mL; 20 μL), eucalyptus oil (10 μL), and tea tree oil (10 μL) mixture at different pH values

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