[논문]금강 상류와 태안, 순천만 해변들로부터 야생효모의 분리 및 국내 미기록 효모들의 균학적 특성

박선정; 장지은; 이향범; 이종수

doi:10.4489/kjm.20210007

금강 상류와 태안, 순천만 해변들로부터 야생효모의 분리 및 국내 미기록 효모들의 균학적 특성
Isolation of Wild Yeasts from Riversides in Geumgang Upstream, Taean and Suncheonman Seashores and Microbiological Characteristics of the Unrecorded Wild Yeasts 원문보기

한국균학회지 = The Korean journal of mycology, v.49 no.1, 2021년, pp.67 - 79

박선정 (배재대학교 바이오의약학부) , 장지은 (배재대학교 바이오의약학부) , 이향범 (전남대학교 응용생물공학부) , 이종수 (배재대학교 바이오의약학부)

초록
AI-Helper

금강 상류와 태안, 순천만 등 해안주변환경의 야생효모들의 효모 종 분포특성을 조사하고 국내 미발표된 효모들을 선별하여 이들의 균학적 특성들을 알아보고자 금강 상류인 충남 금산의 천내강과 서해안에 있는 청포대 해수욕장 및 순천만 습지대 주변의 물과 토양들로부터 야생효모들을 분리, 동정하였고 이들 중 국내 미기록 효모들을 선별하여 이들의 균학적 특성을 조사하였다. 금산의 천내강 주변 물과 토양 40점에서 49종의 야생효모들을 분리, 동정하였고, 태안의 청포대 해수욕장과 순천만 습지대 주변 물과 식물체 부식물 및 토양 80점에서 각각 56종, 36종의 야생효모 들을 분리, 동정하였다. 이들지역에서분리, 동정한야생효모들중C. michaelii NNIBRFG28278, Sp. japonicus NNIBRFG28271, D. buhagiarii NNIBRFG28279, U. spermophora NNIBRFG28273, N. pomicola NNIBRFG31590, C. natalensis NNIBRFG31591, C. pseudorhagii NNIBRFG31592, C. santamariae NNIBRFG31593, Cu. terricola NNIBRFG31594, M. nashicola NNIBRFG31595등 10균주들이 국내 미기록 야생효모들로 최종 선별되었다. 이들 미기록 효모들은C. pseudorhagii NNIBRFG31592과 Cu. terricola NNIBRFG31594 만이 구형이고 나머지 균들은 난형으로 Sp. japonicus NNIBRFG28271와 C. natalensis NNIBRFG31591 두 균주만이 포자를 생성하였다. U. spermophora NNIBRFG28273균주는 vitamin-free배지에서도 생육이 아주 양호하였고 Sp. japonicus NNIBRFG28271균주는 15% NaCl을 함유한 YPD배지에서도 생육이 양호한 호염성균이었다.

Abstract ▼ AI-Helper

This study aimed to isolate wild yeasts from waters and soils on the riversides of Geumgang upstream in Geumsan, seashores of Cheongpodae in Taean and Suncheonman in Suncheon, Korea, and to investigate their unrecorded microbiological characteristics. Forty-nine species of wild yeasts, including Aureobasidium pullans YH 4-3, were isolated from 40 samples of Cheonnaegang in Geumgang upstream. Fifty-six species and 36 species of wild yeasts were also isolated from 80 samples of Cheongpodae Beach and Suncheonman Mashland, respectively. Species from Candida genus were isolated from all three locations. Among them, Candida michaelii NNIBRFG28278, Sporobolomyces japonicus NNIBRFG28271, Dioszegia buhagiarii NNIBRFG28279, Ustilago spermophora NNIBRFG28273, Nakazawaea pomicola NNIBRFG31590, Candida natalensis NNIBRFG31591, Candida pseudorhagii NNIBRFG31592, Candida santamariae NNIBRFG31593, Cutaneotrichosporon terricola NNIBRFG31594, and Meira nashicola NNIBRFG31595 represent newly recorded yeast strains in Korea. Almost all of these unrecorded yeasts were oval shaped, and Sporobolomyces japonicus NNIBRFG28271 and Candida natalensis NNIBRFG31591 have ascospores. All the strains grew well in yeast extract-peptone-dextrose (YPD) and yeast extract-malt extract (YM) media, and Ustilago spermophora NNIBRFG28273 grew well in vitamin-free medium. Sporobolomyces japonicus NNIBRFG28271 grew well in 15% NaCl-containing YPD medium, and Ustilago spermophora NNIBRFG28273 and Dioszegia buhagiarii NNIBRFG28279 assimilated starch, and Ustilago spermophora NNIBRFG28273 produced ethanol.

주제어

표/그림 (9)

표 Table 1. Isolated wild yeasts from freshwaters and soils in riversides of Cheonaegang in Geumgang upstream of Geumsan-gun, Chungcheongnam-do, Korea.
표 Table 2. Isolated wild yeasts from freshwaters and soils in seashore of Cheongpodae beach of Taean, Chungcheongnam-do, Korea. (to be continued)
표 Table 2. Isolated wild yeasts from freshwaters and soils in seashore of Cheongpodae beach of Taean, Chungcheongnam-do, Korea.
표 Table 3. Isolated wild yeasts from freshwaters and soils in Suncheonman marshland of Jeollanam-do, Korea.
표 Table 4. Microbiological characteristics of unrecorded wild yeasts from Cheonnaegang of Geumsan-gun, Cheonpodae beach of Taean and Suncheonman marshland in Korea.
표 Table 4. Microbiological characteristics of unrecorded wild yeasts from Cheonnaegang of Geumsan-gun, Cheonpodae beach of Taean and Suncheonman marshland in Korea.
그림 Fig. 1. Phylogenetic tree of the unrecorded yeasts isolated from freshwaters and soils of Cheonnaegang in Geumgang upstream, based on the nucleotide sequences of large subunit 26S ribosomal DNA D1/D2 region. The tree was generated by the neighbor-joining method, using MEGA 7. The bar indicates the number of substitutions per position. The new isolates from the present study are shown in bold and its detailed characteristics were described in Table 4.
그림 Fig. 2. Phylogenetic tree of the unrecorded yeasts isolated from freshwaters and soils of Cheongpodae beach in Taean and Suncheonman marshland in Suncheon, based on the nucleotide sequences of large subunit 26S ribosomal DNA D1/D2 region. The tree was generated by the neighbor-joining method, using MEGA 7. The bar indicates the number of substitutions per position. The new isolates from the present study are shown in bold and its detailed characteristics were described in Table 4.
표 Table 5. Assimilation and fermentation on sugars, sugar alcohols and organic acid of unrecorded wild yeasts.

참고문헌 (30)

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원문보기 상세보기
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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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