[논문]염증성 장 질환 동물 모델에서 황금탕과 감초사심탕의 효능 연구

김영광; 문영호

doi:10.22246/jikm.2021.42.3.351

염증성 장 질환 동물 모델에서 황금탕과 감초사심탕의 효능 연구
A Study of the Effectiveness of Hwanggeum-tang and Gamchosasim-tang in the Mice Model of Inflammatory Bowel Disease 원문보기

대한한방내과학회지 = The journal of internal Korean medicine, v.42 no.3, 2021년, pp.351 - 374

김영광 (동신대학교 한의과대학 내과학교실) , 문영호 (동신대학교 한의과대학 내과학교실)

Abstract ▼ AI-Helper

Objectives: This study investigates the mechanism of Hwanggeum-tang (HGT) and Gamchosasim-tang (GST) on inflammatory bowel disease (IBD). Methods: The mice (C57BL/6N) were treated with distilled water and 3% dextran sulfate sodium (DSS) to experimentally induce ulcerative colitis. The mice were divided into 7 groups of (6 mice: normal, negative control, positive control (with sulfasalazine), 4 experimental groups (with HGT and GST, respectively). RAW 264.7 cells were used for cell experiments. The experiment was conducted in two ways: in vitro and in vivo. Results: In the experimental group (HGT, GST) of in vitro experiments, NO production decreased, and significant changes in gene expression and protein activation were observed. The length of the colon recovered in the experimental groups (HGT, GST) of the in vivo experiment was longer than that of the negative control group, and the mucosal barrier was recovered. Sone significant changes in the amount of mRNA expression were partially observed, and significant changes in protein activation also were confirmed. Conclusions: HGT and GST are effective in treating IBD caused by DSS. In the same herbal preparation group, the higher the concentration, the better the experimental effect, and when the same concentration was tested, HGT was more effective than GST. Herbal medicine has a higher antioxidant effect than sulfasalazine, so it is also excellent for cell protection.

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표/그림 (16)

표 Table 1. The Amount and Composition of Hwanggeum-tang (HGT) and Gamchosasim-tang (GST)
표 Table 2. The Sequences of Primers
그림 Fig. 1. Effect of HGT and GST on NO level in RAW 264.7 cells.
표 Table 3. Effect of HGT and GST on NO Level in RAW 264.7 Cells
표 Table 4. Effect of HGT and GST on mRNA (PTGS2, NOS2, IL1B, IL6, TNFA, IL10, HPGD, NQO1, HO1, NFE2L2) Expression Level in RAW 264.7 Cells
그림 Fig. 2. Effect of HGT and GST on mRNA (PTGS2, NOS2, IL1B, IL6, TNFA, IL10, HPGD, NQO1, HO1, NFE2L2) expression level in RAW 264.7 cells. RAW 264.7 cells : treated 50, 100, and 200 μg/ml of HGT and GST with 100 ng/ml LPS for 24 h. NO level were calculated as percentage relative to the control. The results : presented by the mean±S.D from 3 independent experiments (Significance of results - ###: p<0.001 compared to normal, * : p<0.05, ** : p<0.01, *** : p<0.001 compared to control).
표 Table 5. Effect of HGT and GST on Protein (COX-2, STAT3, ERK, JNK, P38, HO-1, NRF2) Expression Level in RAW 264.7 Cells
그림 Fig. 3. Effect of HGT and GST on protein (COX-2, STAT3, ERK, JNK, P38, HO-1, NRF2) expression level in RAW 264.7 cells. RAW 264.7 cells : treated 50, 100, and 200 μg/ml of HGT and GST with 100 ng/ml LPS for 24 h. NO level were calculated as percentage relative to the control. The results : presented by the mean±S.D from 3 independent experiments (Significance of results - ###: p<0.001 compared to normal, * : p<0.05, ** : p<0.01, *** : p<0.001 compared to control).
그림 Fig. 4. Effect of HGT and GST on colon length in DSS-induced colitis model. The result were presented by the mean±S.D (Significance of results - ###: p<0.001 compared to normal, ** : p<0.01, *** : p<0.001 compared to control) (n=6). A : data graphic image, B : photograph image
표 Table 6. Effect of HGT and GST on Colon Lengthin DSS-in duced Colitis Model
그림 Fig. 5. Representative colon sections stained with hematoxylin & eosin (H&E). Magnifications : ×100
표 Table 7. Effect of HGT and GST on mRNA (PTGS2, NOS2, IL1B, IL6, TNFA, IL10, HPGD, NQO1, HO1, NFE2L2) Expression Level in DSS-induced Colitis Model
그림 Fig. 6. Effect of HGT and GST on mRNA (PTGS2, NOS2, IL1B, IL6, TNFA, IL10, HPGD, NQO1, HO1, NFE2L2) expression level in DSS-induced colitis model. The results : presented by the mean±S.D (Significance of results - ### : p<0.001 compared to normal, * : p<0.05, *** : p<0.001 compared to control) (n=6).
표 Table 8. Effect of HGT and GST on Protein (COX-2, STAT3, HO-1, NRF2) Expression Level in DSS-induced Colitis Model
그림 Fig. 7. Effect of HGT and GST on protein (COX-2, STAT3, HO-1, NRF2) expression level in DSS-induced colitis model. The results : presented by the mean±S.D (Significance of results - ###: p<0.001 compared to normal, *** : p<0.001 compared to control) (n=6).
그림 Fig. 8. Schematic diagram of this experiment. Animal : 7 week-old C57BL/6N mice * 3% DSS in drinking water for 7 days ** HGT & GST as oral gavage, 200 and 400 mg/kg for 2 weeks *** HGT & GST unit : μg/ml (DSS : Dextran sulfate sodium, HGT : Hwanggeum-tang, GST : Gamchosasim-tang)

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