[논문]Diversity and Antiaflatoxigenic Activities of Culturable Filamentous Fungi from Deep-Sea Sediments of the South Atlantic Ocean

Zhou, Ying; Gao, Xiujun; Shi, Cuijuan; Li, Mengying; Jia, Wenwen; Shao, Zongze; Yan, Peisheng

doi:10.1080/12298093.2020.1871175

Diversity and Antiaflatoxigenic Activities of Culturable Filamentous Fungi from Deep-Sea Sediments of the South Atlantic Ocean 원문보기

Mycobiology, v.49 no.2, 2021년, pp.151 - 160

Zhou, Ying (School of Marine Science and Technology, Harbin Institute of Technology) , Gao, Xiujun (School of Marine Science and Technology, Harbin Institute of Technology) , Shi, Cuijuan (School of Marine Science and Technology, Harbin Institute of Technology) , Li, Mengying (School of Marine Science and Technology, Harbin Institute of Technology) , Jia, Wenwen (School of Marine Science and Technology, Harbin Institute of Technology) , Shao, Zongze (China Key Laboratory of Marine Genetic Resources, The Third Institute of Oceanography, Ministry of Natural Resources) , Yan, Peisheng (School of Marine Science and Technology, Harbin Institute of Technology)

Abstract ▼ AI-Helper

Despite recent studies, relatively few are known about the diversity of fungal communities in the deep Atlantic Ocean. In this study, we investigated the diversity of fungal communities in 15 different deep-sea sediments from the South Atlantic Ocean with a culture-dependent approach followed by phylogenetic analysis of ITS sequences. A total of 29 fungal strains were isolated from the 15 deep-sea sediments. These strains belong to four fungal genera, including Aspergillus, Cladosporium, Penicillium, and Alternaria. Penicillium, accounting for 44.8% of the total fungal isolates, was a dominant genus. The antiaflatoxigenic activity of these deep-sea fungal isolates was studied. Surprisingly, most of the strains showed moderate to strong antiaflatoxigenic activity. Four isolates, belonging to species of Penicillium polonicum, Penicillium chrysogenum, Aspergillus versicolor, and Cladosporium cladosporioides, could completely inhibit not only the mycelial growth of Aspergillus parasiticus mutant strain NFRI-95, but also the aflatoxin production. To our knowledge, this is the first report to investigate the antiaflatoxigenic activity of culturable deep-sea fungi. Our results provide new insights into the community composition of fungi in the deep South Atlantic Ocean. The high proportion of strains that displayed antiaflatoxigenic activity demonstrates that deep-sea fungi from the Atlantic Ocean are valuable resources for mining bioactive compounds.

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표/그림 (4)

표 Table 1. A list of deep-sea sediment samples with details of their sampling information.
그림 Figure 1. Neighbor-joining phylogenetic trees of fungal strains isolated from the 15 deep-sea sediment samples of the South Atlantic Ocean constructed with ITS rDNA sequences. (A) Penicillium species; (B) Aspergillus species; (C) Cladosporium species; (D) Alternaria species. Bootstrap values (expressed as percentages of 1000 replications) of >50% are shown at branch points.
표 Table 2. Diversity of the fungi isolated from the 15 deep-sea sediment samples of the South Atlantic Ocean using Gause No. 1 medium.
표 Table 3. Fermentation characteristics of the 29 deep-sea fungi and antiaflatoxigenic activities of the supernatants of their liquid cultures.

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