[논문]흰민들레(Taraxacum coreanum)에서 분리한 taraxinic acid의 tyrosinase 활성저해 및 melanin 생성저해 효과

박승일; 윤혜련; 신준호; 이성주; 김도윤; 이환명

doi:10.7732/kjpr.2021.34.4.368

흰민들레(Taraxacum coreanum)에서 분리한 taraxinic acid의 tyrosinase 활성저해 및 melanin 생성저해 효과
Tyrosinase Inhibitory Activity and Melanin Production Inhibitory Activity of Taraxinic Acid from Taraxacum coreanum 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.34 no.4, 2021년, pp.368 - 376

박승일 ((주)씨앤비바이오) , 윤혜련 ((주)씨앤비바이오) , 신준호 (경기도경제과학진흥원 바이오센터) , 이성주 (안양대학교 환경에너지공학과) , 김도윤 (호서대학교 화장품생명공학부) , 이환명 (호서대학교 화장품생명공학부)

초록
AI-Helper

흰민들레 추출물에서 분리한 taraxinic acid, dihydrotaraxinic acid 및 luteolin를 대상으로 화장품의 미백 소재로서의 이용 가능성을 조사하였다. In vitro tyrosinase 저해 활성은 100 ㎍/mL에서 대조군인 ascorbic acid는 42% 저해 활성을 보였으며, luteolin은 48%, taraxinic acid는 29%, 그리고 dihydrotaraxinic acid는 18%를 보여 luteolin이 가장 높게 나타냈다. Taraxinic acid와 dihydrotaraxinic acid는 100 ㎍/mL 이하의 조건에서 B16BL6 멜라닌 형성 세포의 생존율에 영향을 미치지 않았으나, luteolin은 강한 세포 독성을 나타냈다. Taraxinic acid와 dihydrotaraxinic acid가 세포 생존율에 영향을 미치지 않는 농도 범위(10, 50, 100 ㎍/mL)에서 B16BL6 세포 내 멜라닌 생합성 및 tyrosinase 활성을 확인한 결과 농도 의존적으로 억제하는 것으로 확인되었다. 특히 taraxinic acid는 tyrosinase inhibitor로 알려진 arbutin보다 뛰어난 저해 활성을 나타내어, 천연 기능성 미백 화장품 원료로 사용 가능성이 기대된다.

Abstract ▼ AI-Helper

This study was to investigate the Taraxinic acid from Taraxacum coreanum on tyrosinase activity and melanogenesis. In B16BL6 cell, Taraxinic acid did not show cytotoxicity even at concentrations of up to 100 ㎍/mL. In addition, tyrosinase inhibitory activity and melanogenesis inhibitory activity were confirmed by stimulation with α-melanocyte stimulating hormone (α-MSH) in the presence of taraxinic acid. Taraxinic acid was added to cells at concentrations of 10, 50 and 100 ㎍/mL and treated for 48 hours to confirm tyrosinase inhibitory activity and melanin production. The tyrosinase inhibitory activity increased in proportion to the amount of the sample, and showed an inhibitory activity of about 54.5% at a concentration of 50 ㎍/mL. Melanin production decreased in proportion to the sample amount, and it was about 62.2% at the concentration of 10 ㎍/mL. From the above results, it was found that Taraxinic acid had higher tyrosinase activity and melanin synthesis inhibitory activity in melanocyte than arbutin. The results suggest that Taraxinic acid can be utilized in natural whitening cosmetics.

주제어

표/그림 (7)

그림 Fig. 1. The procedure for the isolation of three compounds by chromatography from ethanol extract of Taraxacum coreanum.
표 Table 1. Parameters used for HPLC
그림 Fig. 2. Structures of three compounds from taraxacum extract.
그림 Fig. 3. Tyrosinase inhibitory activities of taraxinic acid, dihydrotaraxinic acid, luteolin and ascorbic acid.
그림 Fig. 4. Effects of taraxinic acid (1), dihydrotaraxinic acid (2) and luteolin (3) on the cell viability in B16BL6 melanocyte by the WST assay. The viability of untreated control cells was defined as 100%. The value represents mean ± SD of three different experiments.
그림 Fig. 5. Inhibitory effect of taraxinic acid (1), dihydrotaraxinic acid (2) and α-MSH (200 nM) on melanin production in B16BL6 melanocytes. The value represents mean ± SD of three different experiments.
그림 Fig. 6. Inhibitory effect of taraxinic acid (1), dihydrotaraxinic acid (2) and α-MSH (200 nM) on n tyrosinase activity in B16BL6 melanocytes. The value represents mean ± SD of three different experiments.

참고문헌 (35)

Ahn, J.H., E.J. Mo, Y.H. Jo, B.Y. Hwang and M.K. Lee. 2019. Two new sesquiterpenes from the roots of Taraxacum coreanum. Chem. Nat. Compd. 55:278-280.

상세보기
Ando, H., Y. Niki, M . Ito, K. Akiyama, M .S. M atsui, D.B. Yarosh and M. Ichihash. 2012. Melanosomes are transferred from melanocytes to keratinocytes through the processes of packaging, release, uptake and dispersion. J. Invest. Dermatol. 132:1222-1229.

상세보기
Baba, K., S. Abe and D. Mizuno. 1981. Antitumor activity of hot water extract of dandelion, Taraxacum officinale correlation between antitumor activity and timing of administration. Yakugaku Zasshi 101:538-543.

상세보기
Blaschek, W., R. Hansel, K. Keller, J. Reichling, H. Rimpler and G. Schneider. 1998. Hagers Handbuch derPharmazeutischen Praxis. Berlin, Heidelberg, NY: Springer Verlag, Folgeband 3, Drogen L-Z, p. 897.
Cabanes, J., S. Chazarra and F. Garxia-Carmona. 1994. Kojic acid, a cosmetic skin whitening agent, is a slow-binding inhibitor of catecholase activity of tyrosinase. J. Pharm. Pharmacol. 46:982-985.

상세보기
Choi, J.H., K.M. Shin, N.Y. Kim, J.P. Hong, Y.S. Lee, H.J. Kim, H.J. Park and K.T. Lee. 2002. Taraxinic acid, a hydrolysate of sesquiterpene lactone glycoside from the Taraxacum coreanum NAKAI, induces the differentiation of human acute promyelocytic leukemia HL-60 cells. Biol. Pharm. Bull. 25:1446-1450.

상세보기
Chung, S.K. 2001. Preparation of Antioxidative and Anti-browning Fraction and Purification of Active Compounds from Chestnut Outer Peel. Korea Patent 1020010057283.
Esatbeyoglu, T., B. Obermair, T. Dorn, K. Siems, G. Rimbach and M. Birringer. 2017. Sesquiterpene lactone composition and cellular Nrf2 induction of Taraxacum officinale leaves and roots and taraxinic acid beta-D-glucopyranosyl ester. J. Med. Food. 20:71-78.

상세보기
Francoeur, A.M. and A. Assalian. 1996. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3:19-25.
Hausen, B.M. 1982. Taraxinic acid 1'-O-beta-D-glucopyranoside, the contact sensitizer of dandelion (Taraxacum officinale Wiggers). Derm Beruf Umwelt. 30:51-53.
Hosoi, J., E. Abe, T. Suda and T. Kuroki. 1985. Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer. Res. 45: 1474-1478.

상세보기
Ishikawa, M., I. Kawase and F. Ishii. 2007. Glycine inhibits melanogenesis in vitro and causes hypopigmentation in vivo. Biol. Pharm. Bull. 30:2031-2036.

상세보기
Kang, J.R. and S.M . Yoo. 2009. A study on effect on skin whitening action in Taraxacum platycarpum fractions. Korean J. Aesthet. Cosmetol. 7:199-206.
Kang, M.J., Y.H. Seo, J.B. Kim, S.R. Shin and K.S. Kim. 2000. The chemical composition of Taraxacum officinale consumed in Korea. Korean J. Soc. Food Sci. 16:182-187.
Kim, D.H. 1995. Antitumor activity of fractions of Taraxaci herba synergistic effect with anticancer drugs. Department of Life and Health Science, MS Thesis, Taejon Univ., Korea.
Kim, D.H., T.S. Park and S.G. Kim. 2019. Whitening and antiwrinkle effect of Spirodela polyrhiza extracts. J. Appl. Biol. Chem. 62:391-398.

원문보기 상세보기
Kim, H.S. 2019. Effect of Calendula arvensis extract on antimelanogenesis and antimicrobial activity. J. Appl. Biol. Chem. 62:137-141.

원문보기 상세보기
Lee, B.W. and D.H. Shin. 1991. Screening of natural antimicrobial plant extract on food spoilage microorganism. Korean J. Food Sci. Technol. 23:200-204.
Lee, E.B., J.K. Kim and O.K. Kim. 1993. The antigastritic effect of Taraxaci herba. Korean J. Pharmacogn. 24:313-318.
Lim, S.M., H.J. Park and Y.J. Cho. 2017. Antioxidative and biological activity of extracts from Orostachys japonicus. J. Appl. Biol. Chem. 60:293-300.

원문보기 상세보기
Mascolo, N., G. Autore, F. Capasso, A. Menghini and M.P. Fasulo. 1987. Biological screening of Italian medicinal plants for antiinflammatory activity. Phytother. Res. 1:28-31.

상세보기
Michalska, K., J. Marciniuk and A. Stojakowska. 2020. A new sesquiterpenoid and further natural products from Taraxacum portentosum Kirschner & Stepanek, an endangered species. Nat. Prod. Res. 13:1-5.
Ngamwongsatit, P., P.P. Banada, W. Panbangred and A.K. Bhunia. 2008. WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line. J. Microbiol. Methods 73:211-215.

상세보기
Park, C.I., J.H. Kim, W.S. Hwang, B.D. Lee and K.Y. Lee. 2016. In vitro antityrosinase activity of viscumneoside III and homoflavoyadorinin B isolated from Korean Mistletoe (Viscum album). Korean J. Plant Res. 29:690-698.

원문보기 상세보기
Shahidi, F., P.K. Janitha and P.D. Wanasundara. 1992. Phenolic antioxidants. Critical. Rev. Food Sci. Nut. 32:67-103.

상세보기
Shosuke, I. 2003. A Chemist's view of melanogenesis. Pigment Cell Res. 16:230-236.

상세보기
Slominski, A., D.J. Tobin, S. Shibahara and J. Wortsman. 2004. Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol. Rev. 84:1155-1228.

상세보기
Song, H.N. and N.I. Baek. 2020. Isolation of bioactive compounds from the ethylacetate fraction of fermented garlic complex and their tyrosinase inhibition activities. Korean J. Plant Res. 33:63-72.
Takasaki, M., T. Konoshima, H. Tokuda, K. Masuda, Y. Arai, K. Shiojima and H. Ageta. 1999a. Anticarcinogenic activity of Taraxacum plant. I. Biol. Pharm. Bull. 22:602-605.

상세보기
Takasaki, M., T. Konoshima, H. Tokuda, K. Masuda, Y. Arai, K. Shiojima and H. Ageta. 1999b. Anticarcinogenic activity of Taraxacum plant. II. Biol. Pharm. Bull. 22:606-610.

상세보기
Taylor, S.C. 2002. Skin of color: Biology, structure, function and implications for dermatologic disease. J. Am. Acad. Dermatol. 46:41-62.
Tomita, K., N. Oda, M. Ohbayashi, H. Kamei, T. Miyaki and T. Oki. 1990. A new screening method for melanin biological synthesis inhibitor using Streptomyces bikiniensis. J. Antibiot. 43:1601-1605.
Williams, C.A., F. Goldstone and J. Greenham. 1996. Flavonoids, cinnamic acids and coumarins from the different tissues and medicinal preparations of Taraxacum officinale. Phytochemistry 42:121-127.

상세보기
Yan, Y., J.H. Chu, H. Wang, H. Xu, G.X. Chou, A.K.M. Leung, W.F. Fong and Z.L. Yu. 2010. Involvement of p38 MAPK signaling pathway in the antimelanogenic effect of sanbaitang, a chinese herbal formula, in B16 cells. J. Ethnopharmacol. 132:533-535.

상세보기
Yang, B.C. 2000. Cosmetic composition for skin whitening comprising and the extract from Rubus coreanus Miq. Korea Patent 1020000073267.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증