[논문]3종 한약 처방 물 추출물과 황금 에탄올 추출물에 의한 자궁내막과 태반세포 기능조절 연구

박서예; 노의정; 서창섭; 이성기; 신현규

doi:10.15204/jkobgy.2021.34.3.001

3종 한약 처방 물 추출물과 황금 에탄올 추출물에 의한 자궁내막과 태반세포 기능조절 연구
A Study on the Regulation of Endometrial and Placental Cell Function by Water Extract of 3 Types of Herbal Medicines and Ethanol Extract on Scutellariae Radix 원문보기

大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, v.34 no.3, 2021년, pp.1 - 14

박서예 (산부인과, 건양대학교 의과대학) , 노의정 (산부인과, 건양대학교 의과대학) , 서창섭 (한약연구부, 한국한의학연구원) , 이성기 (산부인과, 건양대학교 의과대학) , 신현규 (한약연구부, 한국한의학연구원)

Abstract ▼ AI-Helper

Methods: We investigated the ability to induce decidualization of human endometrial stromal cells and invasive ability of human trophoblast cells by water extract of three types herbal medicines (Dangguijakyak-san, Siryung-tang, Antae-eum) and ethanol extract on Scutellariae Radix. Results: In the process of decidualization of endometrial stromal cells, three herbal medicines including Dangguijakyak-san, Siryung-tang, and Scutellariae Radix increased the production of decidual markers such as prolactin (PRL) and insulin-like growth factor-binding protein 1 (IGFBP1). However, Antae-eum increased mRNA levels of PRL and IGFBP and secretion of IGFBP in decidual stromal cells, but not PRL. Four herbal medicines inhibited the invasion of trophoblast cells. Conclusions: Four herbal medicines may play a role in implantation in women with reproductive failures. However, further studies are warranted to elucidate whether these medications are helpful in the maintenance of pregnancy.

주제어

표/그림 (7)

표 Table 1. Composition of Each Herbal Medicine Prescription
그림 Fig. 1. Effects of cAMP concentration on decidualization of endometrial stromal cells. T-HESCs were treated with estradiol (10 nM), progesterone (1 μM), and various concentration cAMP for 5 days, and cell viability and PRL expression level confirmed using WST assay and ELISA (†: cyclic adenosine monophosphate).
그림 Fig. 2. Effects of cAMP and four herbal medicine on cell viability in endometrial stromal cells. T-HESCs were treated with estradiol (10 nM), progesterone (1 μM), cAMP (500 μM), and indicated concentration herbal medicines for 5 days, and the survival and growth inhibition of cells were measured through WST assay (†: cyclic adenosine monophosphate, ‡: Dangguijakyak-san, §: Siryung-tang, ∥: Antae-eum, ¶: Scutellariae Radix).
그림 Fig. 3. Effects of four herbal medicine on decidualization control ability at the RNA level in the endometrial stromal cells. T-HESCs were treated with estradiol (10 nM), progesterone (1 μM), cAMP (500 μM), and indicated concentration herbal medicines for 5 days, and expression level of PRL and IGFBP1 confirmed using PCR. (A) PRL, (B) IGFBP1 (*P<0.05, **P<0.01, ***P<0.001, †: cyclic adenosine monophosphate, ‡: Dangguijakyak-san, §: Siryung-tang, ∥: Antae-eum, ¶: Scutellariae Radix).
그림 Fig. 4. Effects of four herbal medicine on decidualization control ability at the protein level in the endometrial stromal cells. T-HESCs were treated with estradiol (10 nM), progesterone (1 μM), cAMP (500 μM), and indicated concentration herbal medicines for 5 days, and expression level of PRL and IGFBP1 confirmed using ELISA. (A) PRL, (B) IGFBP1 (*P<0.05, **P<0.01, ***P<0.001, ‡: Dangguijakyak-san, §: Siryung-tang, ∥: Antae-eum, ¶: Scutellariae Radix, ++: not significant).
그림 Fig. 5. Effects of four herbal medicine on cell viability in trophoblast cells. SW71 cells were treated with various concentration of herbal medicines for 24 hr, the survival and growth inhibition of cells were measured through WST assay. (A) Dangguijakyak-san, (B) Siryung-tang, (C) Antae-eum, (D) Scutellariae Radix (*P<0.05, **P<0.01, ***P<0.001).
그림 Fig. 6. Effects of four herbal medicine on invasive ability of trophoblast cells. SW71 cells were treated with indicated concentration of herbal medicines for 24 hr, and invasive ability of trophoblast cells were measured through invasion assay (*P<0.05, ***P<0.001, ‡: Dangguijakyak-san, §: Siryung-tang, ∥: Antae-eum, ¶: Scutellariae Radix).

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