[논문]임신 관련 3종 한약 처방 물 추출물과 목단피 에탄올 추출물이 자궁내막과 태반세포에 미치는 영향

박서예; 노의정; 서창섭; 이성기; 신현규

doi:10.15204/jkobgy.2021.34.4.030

임신 관련 3종 한약 처방 물 추출물과 목단피 에탄올 추출물이 자궁내막과 태반세포에 미치는 영향
The Effects of Pregnancy-Related Water Extract of 3 Types Herbal Medicines and Ethanol Extract of Moutan Radicis Cortex on the Endometrial and Placental Cells 원문보기

大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, v.34 no.4, 2021년, pp.30 - 45

박서예 (산부인과, 건양대학교 의과대학) , 노의정 (산부인과, 건양대학교 의과대학) , 서창섭 (한의과학연구부, 한국한의학연구원) , 이성기 (산부인과, 건양대학교 의과대학) , 신현규 (한의과학연구부, 한국한의학연구원)

Abstract ▼ AI-Helper

Objectives: This study was performed to investigate the effects of pregnancy-related four herbal medicines (Samul-tang, Onkyung-tang, Chokyungjongok-tang and Moutan Radicis Cortex) on the endometrial and placental cells. Methods: In this study, we examined viability and decidualization of telomerase immortalized human endometrial stromal cell lines (T-HESCs) and viability and invasion ability of human first trimester trophoblast cell lines Sw.71 by four herbal medicines (Samul-tang, Onkyung-tang, Chokyungjongok-tang and Moutan Radicis Cortex) Results: In the study, we showed that Samul-tang, Onkyung-tang, Chokyungjongok-tang increased decidualization marker prolactin (PRL) and insulin-like growth factor-binding protein 1 (IGFBP1) in T-HESCs. Moutan Radicis Cortex decreased the mRNA level of PRL and IGFBP1, and the protein level of PRL and IGFBP1 had no significant effect. Moreover, four herbal medicines reduced invasion ability of Sw.71 cells. Conclusions: These results suggest that Samul-tang, Onkyung-tang, and Chokyungjongok-tang have beneficial effects on successful embryo implantation and pregnancy maintenance by increasing decidualization markers such as PRL and IGFBP1. Moutan Radicis Cortex reduces the mRNA levels of PRL and IGFBP1, which may adversely affect pregnancy. Further investigations are needed to elucidate the significance of the decreased invasive ability of Sw.71 cells induced by four herbal medications.

주제어

표/그림 (6)

표 Table 1. Composition of the 3 Herbal Medicine Formulas
그림 Fig. 1. Effects of 4 types herbal medicines on cytotoxicity of endometrial stromal cells. Treatment with 10 nM estradiol, 1 μM progesterone, 500 μM cAMP and indicated concentration of 4 types herbal medicines for 5 days resulted in no significant effect on the viability of T-HESCs. Cell viability was measured by WST assay. (†; cyclic adenosine monophosphate, ‡; Samul-tang, § ; Onkyung-tang, ∥; Chokyungjongok-tang, ¶ ; Moutan Radicis Cortex)
그림 Fig. 2. Effects of 4 types herbal medicines on decidualization of endometrial stromal cells at the mRNA level. The T-HESCs were treated with 10 nM estradiol, 1 μM progesterone, 500 μM cAMP and 4 types herbal medicines of indicated concentrations for 5 days. PRL and IGFBP1 expression of mRNA level were measured by real-time PCR. (A) PRL, (B) IGFBP1 (*P<0.05, **P<0.01, ***P<0.001, †; cyclic adenosine monophosphate, ‡; Samul-tang, § ; Onkyung-tang, ∥; Chokyungjongok-tang, ¶ ; Moutan Radicis Cortex)
그림 Fig. 3. Effects of 4 types herbal medicines on decidualization of endometrial stromal cells at the protein level. The T-HESCs were treated with 10 nM estradiol, 1 μM progesterone, 500 μM cAMP and 4 types herbal medicines of indicated concentrations for 5 days. PRL and IGFBP1 expression of protein level were measured by ELISA. (A) PRL, (B) IGFBP1 (*P<0.05, ***P<0.001, †; cyclic adenosine monophosphate, ‡; Samul-tang, § ; Onkyung-tang, ∥; Chokyungjongok-tang, ¶ ; Moutan Radicis Cortex, ††; not significant)
그림 Fig. 4. Effects of 4 types herbal medicines on cytotoxicity of trophoblast cells. Treatment with various concentration of 4 types herbal medicines for 24 hr resulted in no significant effect on the viability of Sw.71 cell. Cell viability was measured by WST assay. (A) Samul-tang, (B) Onkyung-tang, (C) Chokyungjongok-tang, (D) Moutan Radicis Cortex (**P<0.01, ***P<0.001)
그림 Fig. 5. Effects of 4 types herbal medicines on invasion abilities of trophoblast cells. Sw.71 cells were treated with four herbal medicines with different concentrations for 24 hours, and invasion assay was performed to confirm the change in invasion of trophoblast cells according to the herbal medicines. (**P<0.01, ***P<0.001, †; Samul-tang, ‡; Onkyung-tang, § ; Chokyungjongok-tang, ∥; Moutan Radicis Cortex, ¶ ; not significant)

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