[논문]반총산의 항산화, 항염증, 항소양증, 항균효능에 관한 실험 연구

조은진; 조성희; 양승정

doi:10.15204/jkobgy.2021.34.3.029

반총산의 항산화, 항염증, 항소양증, 항균효능에 관한 실험 연구
The Experimental Study on Antioxidant, Anti-inflammatory, Antipruritic and Antibacterial Effects of the Banchong-san (BCS) 원문보기

大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, v.34 no.3, 2021년, pp.29 - 48

조은진 (동신대학교 한의과대학 한방부인과학교실) , 조성희 (동신대학교 한의과대학 한방부인과학교실) , 양승정 (동신대학교 한의과대학 한방부인과학교실)

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Objectives: Banchong-san (BCS) is a herbal formula composed of 13 korean medicinal herbs and is traditionally used to treat inflammatory diseases and pain. The object of this study was to research the antioxidant, anti-inflammatory, antipruritic and antimicrobial effects of the BCS in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Methods: In this experiment, effects of BCS on the following four were measured as follows: (1) Anti-oxidative effects were evaluated by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) Radical scavenging activity, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) Radical scavenging activity. (2) Anti-inflammatory effects were evaluated by the production amount of Reactive oxygen species (ROS), Nitric oxide (NO), Interleukin-1β (IL-1β), Interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), Prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2)(the previous two are "mRNA"), extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinases (p38), inhibitor of nuclear factor kappa B (IκBα), nuclear factor kappa B (NF-κB) (the previous five are "Protein") in LPS-Stimulated RAW 264.7 cells. (3)Antipruritic effects were evaluated by the production amount of histamine, Leukotriene B4 (LTB4), LeukotrieneC4 (LTC4) Levels in phorbol 12-myristate 13-acetate(PMA)/ionomycin-stimulated MC/9 mast cell. (4) Anti-microbial effects were evaluated by the growth suppression of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Aspergillus niger. Results: The following results were obtained through each measurement: (1) DPPH Radical Scavenging Activity, ABTS Radical Scavenging Activity evoked a significant concentration-dependent increase. (2) ROS, NO, IL-1β, IL-6, TNF-α, PGE2 production amount, iNOS, COX-2 mRNA expression were significantly reduced in the BCS extraction group compared with the control group and significantly decreased the amount of ERK, JNK, p38, NF-κB Protein expression. The amount of IκB-α Protein Expression have increased significantly. (3) The amounts of histamine, LTB4, LTC4 were significantly decreased. (4) The antibacterial efficacy, BCS inhibited the growth of Escherichia coli, Pseudomonas aeruginosa at concentrations of 5 ㎍/ml, but did not suppress the growth of staphylococcus aureus and aspergillus niger. Conclusions: The experimental results show that BCS has anti-oxidant, anti-inflammatory, antipruritic and antimicrobial properties.

주제어

표/그림 (26)

표 Table 1. The Prescription of BCS
표 Table 2. The Sequences of Primers for Real-Time Polymerase Chain Reaction
그림 Fig. 1. Cell viability of BCS in RAW 264.7 cell.
그림 Fig. 2. Cell viability of BCS in MC/9 cell.
그림 Fig. 3. DPPH radical scavenging activity of BCS.
그림 Fig. 4. ABTS radical scavenging activity of BCS.
그림 Fig. 5. The effects of BCS on ROS production in LPS-stimulated RAW 264.7 cells. ^###p<0.001 compared with the normal group and ***p<0.001 compared with the control group.
그림 Fig. 6. The effects of BCS on NO production in LPS-stimulated RAW 264.7 cells. ^###p<0.001 compared with the normal group and ***p<0.001 compared with the control group.
그림 Fig. 7. Effects of BCS on IL-1β level in LPS-stimulated RAW 264.7 cells. ^###p<0.001 compared with the normal group. And ***p<0.001, **p<0.01, *p<0.05 compared with the control group.
그림 Fig. 8. Effects of BCS on IL-6 level in LPS-stimulated RAW 264.7 cells. ^###p<0.001 compared with the normal group. And ***p<0.001, **p<0.01 compared with the control group.
그림 Fig. 9. The effects of BCS on TNF-α level in LPS-stimulated RAW 264.7 cells. ^###p<0.001 compared with the normal group and ***p<0.001 compared with the control group.
그림 Fig. 10. The effects of BCS on PGE2 producion in LPS-stimulated RAW 264.7 cells. ^###p<0.001 compared with the normal group and **p<0.01, *p<0.05 compared with the control group.
그림 Fig. 11. Effects of BCS on iNOS mRNA expression in LPS-stimulated RAW 264.7 cells. ^###p<0.05 compared with the normal group and **p<0.001, *p<0.05 compared with the control group.
그림 Fig. 12. Effects of BCS on COX-2 mRNA expression in LPS-stimulated RAW 264.7 cells. ^###p<0.001 compared with the normal group and ***p<0.001 compared with the control group.
그림 Fig. 13. The effects of BCS on ERK protein expression in LPS-stimulated RAW 264.7 cells. ^###p<0.01 compared with the normal group and ***p<0.001 compared with the control group.
그림 Fig. 14. The effects of BCS on JNK protein expression in LPS-stimulated RAW 264.7 cells. ^###p<0.001 compared with the normal group and ***p<0.001, **p<0.01 compared with the control group.
그림 Fig. 15. The effects of BCS on p38 protein expression in LPS-stimulated RAW 264.7 cells. ^###p<0.001 compared with the normal group and ***p<0.001 compared with the control group.
그림 Fig. 16. The effects of BCS on IκBa protein expression in LPS-stimulated RAW 264.7 cells. ^###p<0.001 compared with the normal group and ***p<0.001, **p<0.01, *p<0.05 compared with the control group.
그림 Fig. 17. The effects of BCS on NF-κB protein expression in LPS-stimulated RAW 264.7 cells. ^###p<0.001 compared with the normal group and ***p<0.001, **p<0.01, *p<0.05 compared with the control group.
그림 Fig. 18. The effects of BCS on histamine level in PMA/ionomycin-stimulated MC/9 cell. ^###p<0.001 compared with the normal group and ***p<0.001, *p<0.05 compared with the control group.
그림 Fig. 19. The effects of BCS on LTB₄ level in PMA/ionomycin-stimulated MC/9 cell. ^###p<0.001 compared with the normal group and ***p<0.001, **p<0.01 compared with the control group.
그림 Fig. 20. The effects of BCS on LTC₄ level in PMA/ionomycin-stimulated MC/9 cell. ^###p<0.001 compared with the normal group and ***p<0.001, **p<0.01 compared with the control group.
그림 Fig. 21. The inhibition effects of BCS on Escherichia coli growth.
그림 Fig. 22. The inhibition effects of BCS on Pseudomonas aeruginosa growth.
그림 Fig. 23. The inhibition effects of BCS on Staphylococcus aureus growth.
그림 Fig. 24. The inhibition effects of BCS on Aspergillus niger growth.

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