[논문]Reticulitermes Speratus 군체의 모니터링을 위한 염색 시약 선정 및 이종 마킹을 통한 군체 간 식별

IM, Ik-Gyun; HAN, Gyu-Seong

doi:10.5658/wood.2021.49.5.514

[국내논문] Reticulitermes Speratus 군체의 모니터링을 위한 염색 시약 선정 및 이종 마킹을 통한 군체 간 식별
Selection of Dye Markers for Monitoring Reticulitermes speratus and Identification of Colonies by Heterogeneous Dye-Marking 원문보기

목재공학 = Journal of the Korean wood science and technology, v.49 no.5, 2021년, pp.514 - 534

IM, Ik-Gyun (Department of Cultural Heritage Science, Chungbuk National University) , HAN, Gyu-Seong (Department of Wood and Paper Science, Chungbuk National University)

초록
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국내 목조건축물을 가해하는 지중 흰개미인 Reticulitermes speratus 군체의 영역 범위 산정을 위한 적정 염색약 선정 및 확산 경향 평가를 실시하였다. Sawdust diet를 이용한 감수성 평가 결과, 염색약의 농도가 증가할수록 섭식량은 감소하지만 보다 진하게 염색되었으며, 생존율은 Neutral Red 0.5%를 제외하고 4주차까지 모든 농도 조건이 대조군과 차이 없이 85% 이상의 생존하였다. 또한 2가지 염색약 모두 0.1% 농도 조건을 제외한 0.2% 이상의 농도 조건에서 11주 동안 염색이 유지되었고 전이력은 염색약 섭식 직후를 제외하고는 없는 것으로 확인되었다. 이에 따라 Nile Blue A와 Neutral Red 모두 0.2% 농도 조건이 R. speratus 군체의 영역 모니터링에 최적의 농도라고 판단된다. 이와 더불어 실내 조건에서 제작된 40m 길이의 foraging arena에 약 25,000마리 군체를 서식시킨 뒤, Nile Blue A와 Neutral Red 0.2% 농도로 각각 염색된 개체를 양 쪽 끝에 방사하여 시간 경과에 따른 확산 경향을 파악하였다. 그 결과 염색 개체가 7일 정도의 기간에 40m 거리까지 점진적으로 확산 이동하여 서로 섞이는 것이 확인되었다. 이를 통해 목조건축물에 유입된 R. speratus 군체를 대상으로 Mark-Release-Recapture(MRR) 법을 이용한 모니터링을 실시할 경우, 군체의 영역 범위를 산정할 수 있으며, 서로 다른 군체의 식별도 가능할 것이다.

Abstract ▼ AI-Helper

To estimate the territorial size of Reticulitermes speratus, a species of subterranean termites that damages wooden structures in Korea, appropriate dye markers were selected and the diffusion tendency was analyzed. According to the results of the sensitivity assessment using sawdust diet, increase in dye concentration reduced the amount of feed consumption but the dyeing becomes more intense. Except for the Neutral Red 0.5% group, all other concentration groups, including the control group, showed a survival rate of > 85% until week 4. For both, Nile Blue A and Neutral Red dye markers, all concentration conditions > 0.2%, except for the 0.1% concentration, were maintained for 11 weeks, and no sign of transfer effect was found except when the termites were fed with the dye markers. Therefore, it appears that 0.2% of Nile Blue A and Neutral Red is the optimal concentration for monitoring the R. speratus colonies. Additionally, we prepared an indoor 40 m long foraging arena consisting of a colony of 25,000 termites and released individuals stained with 0.2% of Nile Blue A and Neutral Red at both ends to assess the diffusion trends over time. The results showed that it took approximately 7 days for each dyed individual to gradually cover the 40 m distance and mix with each other. These findings reveal that when mark-release-recapture monitoring is conducted on the R. speratus colonies damaging wooden structures, the extent of the colony's activity area could be measured and different colonies could be distinguished from each other.

Keyword

표/그림 (13)

그림 Fig. 1. Foraging arena for estimating the activity area (A: Release point of Nile blue A dyed individuals / B: Release point of Neutral Red dyed individuals).
그림 Fig. 2. Pathway of foraging arena for dyed termite (A: Before termite introduction / B: After termite introduction).
그림 Fig. 3. Marking pattern according to the concentration of Nile Blue A and Neutral Red Deviation (A: Control B: 0.1% C: 0.2% D: 0.5% / Left: Nile Blue A, Right: Neutral Red).
그림 Fig. 4. Marking of soldier due to trophallaxis immediately after feeding (A: Nile Blue A / B: Neutral Red).
그림 Fig. 5. Feed consumption (mean ± SD) according to the concentration of the marking reagent (ANOVA, Tukey post hoc test, α = 0.05).
그림 Fig. 6. Survival units (mean ± SD) for 4 weeks according to the concentration of Nile Blue A (ANOVA, Tukey post hoc test, α = 0.05).
그림 Fig. 7. Survival units (mean ± SD) for 4 weeks according to the concentration of Neutral Red (ANOVA, Tukey post hoc test, α = 0.05).
그림 Fig. 8. Comparison of dye marking patterns by concentration conditions over time (A-C: initial, D-F: 4weeks later, G-I: 8weeks later, J-L: 11weeks later / Left: Nile Blue A, Right: Neutral Red).
표 Table 1. Change in the numbers of dyed and undyed R. speratus workers after mixing thirty workers fed Nile Blue A or Neutral Red
그림 Fig. 9. Molting of dyed worker (Left: Nile blue A / Right: Neutral Red).
그림 Fig. 10. Foraging range of marked individuals over time.
그림 Fig. 11. Foraging pattern of the released dyed worker.
표 Table 2. Comparing the results of a prior study in the foraging distance according to the species of Reticulitermes spp.

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