고함량 사포나린 함유 보리 어린 순 재배를 위한 식물공장내 인공광 조건 및 사포나린 추출 분석법 최적화 Optimization of the extraction procedure for quantitative analysis of saponarin and the artificial light condition for saponarin production from barley sprout원문보기
본 연구에서는 보리 어린 순의 기능성 성분인 saponarin의 정량적 추출을 위한 최적의 추출 분석 조건을 methanol, ethanol, acetonitrile 및 water를 대상으로 반응표면분석법을 통해 실시하였다. 이를 통해 용매의 농도, 추출 시간에 따른 saponarin과 색소 추출율을 확인한 결과, 53.7% methanol 수용액에서 3.9 h 동안 진탕추출하는 것이 높은 saponarin 추출율을 유지하면서, 불필요한 색소의 추출을 최소화하는 최적 추출조건으로 확인되었다. 보리 어린 순의 재배에 필요한 인공광의 saponarin 생성 영향 평가를 광 주기, 광량, 광질을 달리한 조건에서 시험한 결과, saponarin 고함유 보리 어린 순의 인공광 최적 재배 조건은 8 h day-1의 광 주기에서 6500K LED와 적색보광을 통한 총 광량 220-320 μmol m-2 s-1 에서 가장 효과적임을 확인 할 수 있었고, 청색광이 saponarin 생합성에 주요 인자로 작용함을 확인할 수 있었다.
본 연구에서는 보리 어린 순의 기능성 성분인 saponarin의 정량적 추출을 위한 최적의 추출 분석 조건을 methanol, ethanol, acetonitrile 및 water를 대상으로 반응표면분석법을 통해 실시하였다. 이를 통해 용매의 농도, 추출 시간에 따른 saponarin과 색소 추출율을 확인한 결과, 53.7% methanol 수용액에서 3.9 h 동안 진탕추출하는 것이 높은 saponarin 추출율을 유지하면서, 불필요한 색소의 추출을 최소화하는 최적 추출조건으로 확인되었다. 보리 어린 순의 재배에 필요한 인공광의 saponarin 생성 영향 평가를 광 주기, 광량, 광질을 달리한 조건에서 시험한 결과, saponarin 고함유 보리 어린 순의 인공광 최적 재배 조건은 8 h day-1의 광 주기에서 6500K LED와 적색보광을 통한 총 광량 220-320 μmol m-2 s-1 에서 가장 효과적임을 확인 할 수 있었고, 청색광이 saponarin 생합성에 주요 인자로 작용함을 확인할 수 있었다.
Saponarin is a crucial component of barley sprout, and the production and quantitative analysis are issued to date. In this study, the optimal saponarin extraction conditions were presented on the subject of acetonitrile, ethanol, methanol, and water for the quantitative analysis in barley sprout th...
Saponarin is a crucial component of barley sprout, and the production and quantitative analysis are issued to date. In this study, the optimal saponarin extraction conditions were presented on the subject of acetonitrile, ethanol, methanol, and water for the quantitative analysis in barley sprout through the extraction efficiency compared with the solvent concentration and extraction time using the reaction surface methodology. The optimal extraction time and solvent condition for saponarin were 3.9 h and 53.7% of aqueous methanol, respectively. In addition, the effect of LED artificial light on the saponarin production in barley sprouts was evaluated by the light cycle, light quantity, and light quality. The optimal cultivation conditions under artificial light for the growth of barley sprout and saponarin production were most effectively achieved on 220-320 μmol m-2 s-1 of the light quantity with 8 h day-1 of a daylight cycle under 6500K LED combined with red light. Furthermore, blue light was evaluated as the main factor in the biosynthesis of saponarin.
Saponarin is a crucial component of barley sprout, and the production and quantitative analysis are issued to date. In this study, the optimal saponarin extraction conditions were presented on the subject of acetonitrile, ethanol, methanol, and water for the quantitative analysis in barley sprout through the extraction efficiency compared with the solvent concentration and extraction time using the reaction surface methodology. The optimal extraction time and solvent condition for saponarin were 3.9 h and 53.7% of aqueous methanol, respectively. In addition, the effect of LED artificial light on the saponarin production in barley sprouts was evaluated by the light cycle, light quantity, and light quality. The optimal cultivation conditions under artificial light for the growth of barley sprout and saponarin production were most effectively achieved on 220-320 μmol m-2 s-1 of the light quantity with 8 h day-1 of a daylight cycle under 6500K LED combined with red light. Furthermore, blue light was evaluated as the main factor in the biosynthesis of saponarin.
Lee JJ, Park DH, Lee WY (2017) Optimization of microwave assisted extraction process of Hordeum vulgare L. by response surface methodology. Korean J Food Preserv 24: 949-956. doi: 10.11002/kjfp.2017.24.7.949
Lee YH, Kim JH, Kim SH, Oh JY, Seo WD, Kim KM, Jung JC, Jung YS (2016) Barley sprouts extract attenuates alcoholic fatty liver injury in mice by reducing inflammatory response. Nutrients 8: 440. doi: 10.3390/ nu8070440
Ha KY (2018) Current status on research and industrial value of balrey sprout. Dissertation, Kongju National University
Kim JS (2021) The effect of artificaial lights on the growth and quality of hydroponic cultivated barley (Hordeum vulgare L.) sprouts. J Plant Biotechnol 48: 62-70. doi: 10.5010/JPB.2021.48.1.062
Gupta SD, Jatothu B (2013) Fundamentals and applications of lightemitting diodes LEDs in in vitro plant growth and morphogenesis. Plant Biotechnol Rep 7: 211-220. doi: 10.1007/s11816-013-0277-0
Um YC, Oh SS, Lee JG, Kim SY, Jang YA (2010) The development of container-type plant factory and growth of leafy vegetables as affected by different light sources. J Bio-Env Con 19: 333-342
Lee GI, Kim HJ, Kim SJ, Lee JW, Park JS (2016) Increased growth by LED and accumulation of functional materials by fluorescence lamps in a hydroponics culture system for Angelica gigas. J Bio-Env Con 25: 42-48. doi: 10.12791/KSBEC.2016.25.1.42
Chung SO, Kim YM, Ryu DG, Kim SJ, Park JT (2014) Variation of functional compounds in leafy chinese cabbage grown under different light conditions in a plant factory. Korean J Food Sci Technol 46: 526-529. doi: 10.9721/KJFST.2014.46.4.526
Park S, Cho E, An J, Yoon B, Choi K, Choi E (2019) Plant growth and ascorbic acid content of spinacia oleracea grown under different light-emitting diodes and ultraviolet radiation light of plant factory system. J Bio-Env Con 28: 1-8. doi: 10.12791/KSBEC.2019.28.1.1
Lee HR, Kim HM, Kim HM, Park SH, Hwang SJ (2019) Applicability of artificial light source and newly developed growing medium for lettuce cultivation in a closed-type plant production system. J Bio-Env Con 28: 134-142. doi: 10.12791/KSBEC.2019.28.2.134
Park SY, Oh MM (2021) Enhancement of crepidiastrum denticulatum production using supplemental far-red radiation under various white LED lights. J Bio-Env Con, 30: 149-156. doi: 10.12791/KSBEC.2021.30.2.149
Kim S, Bok G, Lee G, Park J (2017) Growth characteristics of lettuce under different frequency of pulse lighting and RGB ratio of LEDs. J Bio-Env Con 26: 123-132. doi: 10.12791/KSBEC.2017.26.2.123
Kang S, Yang HJ, Ko BS, Kim MJ, Kim BS, Park S (2015) Effect of LED with mixed wavelengths on bio-active compounds in cherry tomato and red cabbage. Korean J Food Cook Sci 31: 505-509. doi: 10.9724/kfcs.2015.31.4.505
Gomes PGC, Veloso AF, Maynard IFN, Marques MN, de Souza RL, Pereira MM, Soares CMF, Lima AS (2020) Integrative process to extract chlorophyll and purify rosmarinic acid from rosemary leaves (Rosmarinus officialis). J Chem Technol Biotechnol 95: 1503-1510. doi: 10.1002/jctb.6343
Kozai T, Kubota C, Chun C, Afreen F, Ohyama K (2000) Necessity and concept of the closed transplant production system. In: Kubota C, Chun C (eds) Transplant production in the 21st century. Springer, Dordrecht, pp 3-19
Neff MM, Van Volkenburgh E (1994) Light-stimulated cotyledon expansion in Arabidopsis seedlings (the role of phytochrome B). Plant Physiol 104: 1027-1032. doi: 10.1104/pp.104.3.1027
Zheng L, Van Labeke MC (2017) Chrysanthemum morphology, photosynthetic efficiency and antioxidant capacity are differentially modified by light quality. Plant Physiol 213: 66-74. doi: 10.1016/j.jplph.2017.03.005
Meng XC, Xing T, Wang XJ (2004) The role of light in the regulation of anthocyanin accumulation in Gerbera hybrida. J Plant Growth Regul 44: 243-250. doi: 10.1007/s10725-004-4454-6
Kim S, Bok G, Park J (2018) Analysis of antioxidant content and growth of Agastache rugosa as Affected by LED light qualities. J Bio-Env Con 27: 260-268. doi: 10.12791/KSBEC.2018.27.3.260
AI-Helper
※ AI-Helper는 부적절한 답변을 할 수 있습니다.