[논문]자생 Tuber himalayense 자실체 추출물의 항염증 활성

김민경; 홍혜현; 김정환; 김승영; 김창무

doi:10.14480/jm.2021.19.3.176

자생 Tuber himalayense 자실체 추출물의 항염증 활성
Anti-inflammatory activity of indigenous Tuber himalayense in Korea 원문보기

Journal of mushrooms = 한국버섯학회지, v.19 no.3, 2021년, pp.176 - 183

김민경 (국립생물자원관 미생물자원과) , 홍혜현 (선문대학교 제약생명공학과) , 김정환 (선문대학교 제약생명공학과) , 김승영 (선문대학교 제약생명공학과) , 김창무 (국립생물자원관 유용자원분석과)

초록
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본 연구는 국내 참나무 생육지에서 채집된 송로버섯의 일종인 T. himalayense 자실체 추출물(TH)의 항염증 활성을 확인하였다. LPS로 유도된 RAW 264.7 대식세포를 대상으로 한 염증 억제 실험에서 TH는 100 ㎍/ml 이하의 농도에서 세포독성을 보이지 않았으며 LPS에 의해 증가된 NO와 PGE₂의 생성을 농도 의존적으로 억제하였다. Western blot 분석 결과로 볼 때, TH의 항염증 활성은 iNOS와 COX-2 유전자의 발현억제에 의해 NO와 PGE₂의 생성이 감소된 것으로부터 유발된 것임을 입증한다. 또한, TH가 대식세포에 의해 생산 및 분비되는 cytokine IL-1β와 IL-6의 생성을 효과적으로 저해함을 확인하였다. T. himalayense는 다양한 염증 매개체들의 과발현을 효과적으로 억제하며, 이를 표적으로 하는 항염증 관련 식품 및 의약품에 다양하게 활용 될 수 있을 것으로 기대된다. 추후 염증 예방 물질로 적용 될 수 있는 가능성을 높이기 위하여 염증성 작용 기전에 대한 자세한 연구가 필요할 것으로 사료된다.

Abstract ▼ AI-Helper

In this study, the anti-inflammatory activity of an extract of the fruiting body of the Tuber himalayense (TH) truffle collected from oak growing areas in Korea was investigated. The extract was not cytotoxic at concentrations below 100 ㎍/mL in an experiment evaluating inflammation inhibitory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production was inhibited by the extract in a concentration-dependent manner. Western blot assay results indicated that the anti-inflammatory activity of TH extract was likely caused by the reduced production of NO and PGE2 via suppression of induced NO synthase and cyclooxygenase-2 gene expression. In addition, TH extract effectively inhibited the production of interleukin (IL)-1β and IL-6 by macrophages. Thus, TH extract effectively inhibits the overexpression of various inflammatory mediators and could be valuable in formulating anti-inflammatory foods and medicines that target these components.

주제어

표/그림 (7)

그림 Fig. 1.Morphological features of Tuber himalayense (NIBRFG0000505337), Ascocarp (A), peridium and gleba (B), asci (C-E), ascospores (F-G), SEM images of ascospores(H-J). Scale bars B = 1 cm, C = 100 μm, D-E = 50 μm, F-G = 10 μm, H-J = 10 μm.
그림 Fig. 2. Phylogenetic tree of Tuber himalayense(NIBRFG0000505337). The tree inferred from concatenated alignment of internal transcribed spacer(ITS) and Large-subunit rRNA(LSU) sequences. The topological structure was determined using a Maximum Likelihood method based on the Kimura 2-parameter model. The sample from our study is indicated in bold. Scale bar indicates the number of substitutions per site for each branch.
그림 Fig. 3. Inhibitory effects of TH on cell viability in RAW 264.7 cells. The production of nitric oxide was assayed in the culture medium of cells stimulated with LPS (1 μg/mL) for 24 h in the presence of TH (25, 50 and 100 μg/mL). Cytotoxicity was determined by MTT assay. Result is expressed as percentages compared to the respective values obtained for the control.
그림 Fig. 4. Inhibitory effects of TH on nitric oxide production in RAW 264.7 cells. The production of nitric oxide was assayed in the culture medium of cells stimulated with LPS (1 μg/ mL) for 24 h in the presence of TH (25, 50 and 100 μg/mL). Result is expressed as percentages compared to the respective values obtained for the control. Data represent the means ± SD with three separate experiments. *p<0.05; **p<0.01.
그림 Fig. 5. Inhibitory effects of TH on PGE₂ production in RAW 264.7 cells. The production of PGE₂ was assayed in the culture medium of cells stimulated with LPS (1 μg/mL) for 24 h in the presence of TH extract (25, 50, and 100 μg/mL). Result is expressed as percentages compared to the respective values obtained for the control. Data represent the means ± SD with three separate experiments. *p<0.05; **p<0.01.
그림 Fig. 6. Inhibitory effects of TH on the protein level of iNOS and COX-2 expression in LPS-stimulated RAW 264.7 cells. Cells 4.0 × 10⁵ cells/well were pre-incubated for 24 h, and then treated with LPS (1 μg/mL) and Sample for 24 h. The protein levels of a) iNOS, b) COX-2 were analyzed by western blot. β-actin was used as the control.
그림 Fig. 7. Inhibitory effects of TH on production of proinflammatory cytokines in RAW 264.7 cells. The production of a) TNF-α b) IL-6, and c) IL-1β was assayed in the culture medium of cells stimulated with LPS (1 μg/mL) for 24 h in the presence of TH (25, 50, and 100 μg/mL). Results are expressed as a percentage of the control. Data represent the means ± SD with three separate experiments. *p<0.05; **p<0.0.

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