[논문]굴피나무 잎 추출물의 위암세포에 대한 세포사멸 유도 효과

이형선

doi:10.48022/mbl.2102.02009

[국내논문] 굴피나무 잎 추출물의 위암세포에 대한 세포사멸 유도 효과
Induction of Apoptosis in AGS Human Gastric Cancer Cells by Platycarya strobilacea Leaf Extract 원문보기

Microbiology and biotechnology letters = 한국미생물·생명공학회지, v.49 no.3, 2021년, pp.283 - 288

이형선 (중원대학교 임상병리학과)

초록
AI-Helper

본 연구에서는 굴피나무 잎 추출물로부터 사람 위암세포주인 AGS 세포에 대한 apoptosis가 유도되어 항암 활성을 가지는지를 확인하고자 한다. AGS 세포에 굴피나무 잎 추출물을 농도별(0, 50, 150, 200 ㎍/ml)로 처리하여 세포에서 나타나는 양상들을 확인하였다. MTS 측정으로 암세포 생존율을 확인한 결과 굴피나무 잎 추출물에 따라 농도의존적으로 세포에 독성을 보였으며, 이러한 세포 죽음이 apoptosis 유도에 의한 것인지를 annexin V/FITC-PI 염색을 통해 확인하였다. 그 결과, early apoptosis를 보이는 세포의 양상이 유의성 있게 증가하였다. 굴피나무 잎 추출물의 처리는 세포 주기에서 sub-G1기의 증가로 암세포가 더 이상 분열하지 못하고 증식이 억제됨을 보여준다. 내인성 경로에 관여하는 Bax, Bak, Bcl-2, Bcl-xL의 증감을 mRNA 수준에서 RT-PCR로 확인한 결과, Bax, Bak가 증가하고, Bcl-2, Bcl-xL이 감소를 동반하며 미토콘드리아를 통한 세포사멸의 신호전달 경로가 진행되고 있음을 확인할 수 있었다. 이러한 결과를 종합하면 굴피나무 잎 추출물은 사람 위암세포에 대한 항암 활성을 가지는 효능적 가치가 있음에 가능성을 제시할 수 있을 것으로 생각되며, 추가적인 동물실험을 통한 인체적용시험의 효능 검증을 통해 의약품 개발 가능성을 확인해야 한다.

Abstract ▼ AI-Helper

This study investigated the anticancer activity of methanol extract from Platycarya strobilacea leaf in AGS human gastric cancer cells. We determined the cell viability effect of P. strobilacea using MTS assay. Apoptosis induction and cell cycle arrest were confirmed by fluorescein isothiocyanate and propidium iodide staining using cellometer K2. The mRNA expression levels of the Bcl-2 family were confirmed by reverse transcription-polymerase chain reaction. The cell viability was decreased in a dose-dependent manner treated with different concentrations of P. strobilacea. Total, early, and late apoptotic cells were dramatically increased, and the cell cycle was arrested at the sub-G1 phase. The mRNA expressions of Bcl-2 and Bcl-xL were reduced, whereas pro-apoptotic factors, Bax and Bak, were increased in a dose-dependent manner. These results suggested that P. strobilacea leaf extract induced significant apoptotic activity through an intrinsic mitochondria pathway.

주제어

표/그림 (4)

그림 Fig. 1. Cell viability of AGS cells by MTS assay. The cells were treated with PSE for 24 h and added with MTS solution for 3 h. Significant evaluation was indicated from control by t-test (*p < 0.05, **p < 0.01, ***p < 0.001).
그림 Fig. 2. Apoptotic activity of AGS cells by annexin V/FITC-PI stain. The cells were treated with PSE (0, 50, 150, and 200 μg/ml) for 15 h, and stained with annexin V/FITC-PI solution for 0.5 h. (A) The cells were determined by cellometer K2. (B) and (C) Percent of early, late and total apoptosis was indicated using bar charts. Significant evaluation was indicated from control by t-test (*p < 0.05, **p < 0.01, ***p < 0.001, n = 6).
그림 Fig. 3. Cell cycle analysis in AGS cells by PI stain. The cells were treated with PSE (0, 50, 150, and 200 μg/ml) for 15 h, fixed in 70% ethanol, and stained with PI solution for 0.5 h.
그림 Fig. 4. mRNA level analysis in AGS cells treated with P. strobilacea extract (0, 50, 150, and 200 μg/ml). And, density of mRNA band was expressed as a value compared to the control. Significant evaluation was indicated from control by t-test (*p <0.05, **p < 0.01, ***p < 0.001, n = 3).

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