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이매패류(Sinonovacula constricta) 먹이원 NGS 분석 적용에 대한 연구
Application of NGS Analysis for the Food Source of Bivalve 원문보기

생태와 환경 = Korean journal of ecology and environment, v.54 no.3, 2021년, pp.257 - 264  

허유지 (순천대학교 환경교육과) ,  조현빈 (부산대학교 환경.에너지연구소) ,  정은송 (부산대학교 생명과학과) ,  김현우 (순천대학교 환경교육과)

초록
AI-Helper 아이콘AI-Helper

본 연구에서는 가리맛조개(S. constricta)의 토사물을 현미경 검경과 차세대염기서열분석(NGS) 기법으로 먹이원을 확인하고, 이를 통해 형태학적 및 분자학적 방법에 따른 먹이원 분석을 비교하였다. 가리맛조개(S. constricta)의 먹이원은 분석방법에 따라 차이를 보였다. 먹이원생물은 위 내에서 분해되어 현미경 분석을 통한 생활사 확인과 정량적 분석이 가능하였으나 형태학적 및 해부학적 특성 파악이 불완전하였다. NGS 분석은 유기물 형태로 잔존하는 생물의 DNA 확인이 가능하여 현미경 검경 결과와의 상호보완적 적용 가능성을 확인하였다.

Abstract AI-Helper 아이콘AI-Helper

In this study, we analyzed the food components in the release product that sampled Sinonovacula constricta from the foreshore littoral at Byeongnyang-myeon, Suncheon Bay. We used microscopy and next-generation sequencing (NGS) to evaluate the applicability of morphological and molecular methods to a...

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참고문헌 (38)

  1. Albaina, A., M. Aguirre, D. Abad, M. Santos and A. Estonba. 2016. 18S rRNA V9 metabarcoding for diet characterization: a critical evaluation with two sympatric zooplanktivorous fish species. Ecology and Evolution 6(6): 1809-1824. 

  2. Alfaro, A.C. 2006. Evidence of cannibalism and bentho-pelagic coupling within the life cycle of the mussel, Perna canaliculus. Journal of Experimental Marine Biology and Ecology 329(2): 206-217. 

  3. Amaral-Zettler, L.A., E.A. McCliment, H.W. Ducklow and S.M. Huse. 2009. A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes. PloS ONE 4(7): e6372. 

  4. Carroll, E.L., R. Gallego, M.A. Sewell, J. Zeldis, L. Ranjard, H.A. Ross and R. Constantine. 2019. Multi-locus DNA metabarcoding of zooplankton communities and scat reveal trophic interactions of a generalist predator. Scientific Reports 9(1): 1-14. 

  5. Cassie, V. 1989. A contribution to the study of New Zealand diatoms. Bibliotheca Diatomologica 17: 1-266, 13 pls. 

  6. Clarke, L.J., J.M. Beard, K.M. Swadling and B.E. Deagle. 2017. Effect of marker choice and thermal cycling protocol on zooplankton DNA metabarcoding studies. Ecology and Evolution 7(3): 873-883. 

  7. Cloern, J.E. 1982. Does the benthos control phytoplankton biomass in south San Francisco Bay. Marine Ecology Progress Series. Oldendorf 9(2): 191-202. 

  8. Davenport, J., D. Ezgeta-Balic, M. Peharda, S. Skejic, Z. Nincevic-Gladan and S. Matijevic. 2011. Size-differential feeding in Pinna nobilis L. (Mollusca: Bivalvia): Exploitation of detritus, phytoplankton and zooplankton. Estuarine Coastal and Shelf Science 92: 246-254. 

  9. Davenport, J., R.J. Smith and M. Packer. 2000. Mussels Mytilus edulis: significant consumers and destroyers of mesozooplankton. Marine Ecology Progress Series 198: 131-137. 

  10. Deagle, B.E., D.J. Tolli, S.N. Jarman, M.A. Hindell, A.W. Trites and N.J. Gales. 2005. Molecular scatology as a tool to study diet: analysis of prey DNA in scats from captive Steller sea lions. Molecular Ecology 14(6): 1831-1842. 

  11. Dubois, S., H. Blanchet, A. Garcia, M. Masse, R. Galois, A. Gremare and N. Savoye. 2014. Trophic resource use by macrozoobenthic primary consumers within a semi-enclosed coastal ecosystem: stable isotope and fatty acid assessment. Journal of Sea Research 88: 87-99. 

  12. Einsle, U. 1993. Crustacea, Copepoda, Calanoida und Cyclopoida. Susswasswefauna von Mitteleuropa, vol. 8, part 4-1. Gustav Fisher Verlag, Stuttugart. 

  13. Feng, B., L. Dong, D. Niu, S. Meng, B. Zhang, D. Liu and J. Li. 2010. Identification of immune genes of the Agamaki clam (Sinonovacula constricta) by sequencing and bioinformatic analysis of ESTs. Marine Biotechnology 12(3): 282-291. 

  14. Foged, N. 1978. Diatoms in eastern Australia. Bibliotheca Phycologica 41: 1-243, 48 pls. 

  15. Guo, K., W. Zhao, Z. Jiang and S. Dong. 2017. A study of organic carbon, nitrogen and phosphorus budget in jellyfish-shellfish-fish-prawn polyculture ponds. Aquaculture Research 48(1): 68-76. 

  16. Jo, H., D.K. Kim, K. Park and I.S. Kwak. 2019. Discrimination of spatial distribution of aquatic organisms in a coastal ecosystem using eDNA. Applied Sciences 9(17): 3450. 

  17. Kharlamenko, V.I., S.I. Kiyashko, A.B. Imbs and D.I. Vyshkvartzev. 2001. Identification of food sources of invertebrates from the seagrass Zostera marina community using carbon and sulfur stable isotope ratio and fatty acid analyses. Marine Ecology Progress Series 220: 103-117. 

  18. Kim, H.J., T.K. Lee, S.W. Jung, I.K. Kwon and J.W. Yoo. 2018. Analyzing Vomit of Platalea minor(Black-faced Spoonbill) to Identify Food Components using Next-Generation Sequencing and Microscopy. Korean Journal of Environmental Biology 36(2): 165-173 (in Korean). 

  19. Kim, H.S. 2016. Phytoplankton variability in digestive tract of the Manila clam Ruditapes philippinarum in Gomso Bay, west coast of Korea. The Korean Journal of Malacology 32(3): 165-174 (in Korean). 

  20. Koh, C.H., M.R. Yang and W.K. Chang. 1997. Growth and production of Sinonovacula constricta (Bivalvia) from the Hwaseong tidal flat in the Namyang Bay, Korea. The Sea 2(1): 21-30 (in Korean). 

  21. Koste, W. 1978. Rotatoria. Die Radertiere Mitteleuropes begrunder von Max Voigt, 2nd edn., Vol. 1. Textband, p. 673, Vol. 2. Tafelband, p. 234, Borntraeger, Stuttgart. 

  22. Lehane, C. and J. Davenport. 2006. A 15-month study of zooplankton ingestion by farmed mussels (Mytilus edulis) in Bantry Bay, Southwest Ireland. Estuarine Coastal and Shelf Science 67: 645-652. 

  23. Li, W., L. Fu, B. Niu, S. Wu and J. Wooley. 2012. Ultrafast clustering algorithms for metagenomic sequence analysis. Briefings in Bioinformatics 13(6): 656-668. 

  24. Lukwambe, B., W. Yang, Y. Zheng, R. Nicholaus, J. Zhu and Z. Zheng. 2018. Bioturbation by the razor clam(Sinonovacula constricta) on the microbial community and enzymatic activities in the sediment of an ecological aquaculture wastewater treatment system. Science of the Total Environment 643: 1098-1107. 

  25. Magoc, T. and S.L. Salzberg. 2011. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27(21): 2957-2963. 

  26. Maoxiao, P., L. Xiaojun, N. Donghong, Y. Bo, L. Tianyi, D. Zhiguo and L. Jiale. 2019. Survival, growth and physiology of marine bivalve (Sinonovacula constricta) in long-term low-salt culture. Scientific Reports 9(1): 1-9. 

  27. McCann, K.S. 2000. The diversity-stability debate. Nature 405(6783): 228-233. 

  28. Na, Y.K., H. Jo, J.W. Park, K.H. Chang and I.S. Kwak. 2020. The Gut Content Analysis of Polypedilum scalaenum in the Large-scale Weirs of 4 Major River Ecosystems. Korean Journal of Ecology and Environment 53(1): 55-62 (in Korean). 

  29. Niu, D., L. Wang, F. Sun, Z. Liu and J. Li. 2013. Development of molecular resources for an intertidal clam, Sinonovacula constricta, using 454 transcriptome sequencing. PLoS ONE 8(7): e67456. 

  30. Niu, D., L. Wang, L.Z. Bai, S. Xie, H. Zhao and J. Li. 2015. Identification and expression characterization of the myostatin (MSTN) gene and association analysis with growth traits in the razor clam Sinonovacula constricta. Gene 555(2): 297-304. 

  31. Round, F.E., R.M. Crawford and D.G. Mann. 1990. The Diatoms: Biology & morphology of the genera. Cambridge [England: Cambridge University Press. 

  32. Schloss, P.D., S.L. Westcott, T. Ryabin, J.R. Hall, M. Hartmann, E.B. Hollister, R.A. Lesniewski, B.B. Oakley, D.H. Parks, C.J. Robinson, J.W. Sahl, B. Stres, G.G. Thallinger, D.J. Van Horn and C.F. Weber. 2009. Introducing mothur: opensource, platform-independent, community-supported software for describing and comparing microbial communities. Applied and Environmental Microbiology 75(23): 7537-7541 

  33. Smirnov, N.N. and B.V. Timms. 1983. A revision of the Australian Cladocera (Crustacean). Records of the Australian Museum Supplement 1: 1-132. 

  34. Suh, J., T. Kim, S. Shin, H.Y. Kahng, S. Ahn, J.S. Jung and N.I. Won. 2017. Geochemical Characteristics and Benthos Distribution in the Three Shellfish Farms in Suncheon Bay, Korea. Journal of Environmental Science International 26(6): 691-710 (in Korean). 

  35. Thomsen, P.F. and E. Willerslev. 2015. Environmental DNA - An emerging tool in conservation for monitoring past and present biodiversity. Biological Conservation 183: 4-18. 

  36. Wong, W.H. and S.G. Cheung. 2001. Feeding rhythms of the green-lipped mussel, Perna viridis(Linnaeus, 1758) (Bivalvia: Mytilidae) during spring and neap tidal cycles. Journal of Experimental Marine Biology and Ecology 257(1): 13-36. 

  37. Yoon, T.H., H.E. Kang, C.K. Kang, S.H. Lee, D.H. Ahn, H. Park and H.W. Kim. 2016. Development of a cost-effective metabarcoding strategy for analysis of the marine phytoplankton community. PeerJ 4: e2115. 

  38. Zhang, Z., S. Schwartz, L. Wagner and W. Miller. 2000. A greedy algorithm for aligning DNA sequences. Journal of Computational Biology 7(1-2): 203-214. 

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