[논문]꽃사과(Malus prunifolia Borkh.) 추출물의 생리활성 평가 및 활성 성분의 규명

신현영; 김훈; 정은진; 김현경; 이경행; 배윤정; 김우중; 이상현; 유광원

doi:10.9799/ksfan.2021.34.5.477

꽃사과(Malus prunifolia Borkh.) 추출물의 생리활성 평가 및 활성 성분의 규명
Evaluation of the Physiological Activity and Identification of the Active Ingredients of Crab Apple (Malus prunifolia Borkh.) Extracts 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.34 no.5, 2021년, pp.477 - 486

신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공) , 김훈 (중앙대학교 생명공학대학) , 정은진 (한국교통대학교 식품영양학전공) , 김현경 (한국교통대학교 식품영양학전공) , 이경행 (한국교통대학교 식품영양학전공) , 배윤정 (한국교통대학교 식품영양학전공) , 김우중 (경기도 경제과학진흥원 바이오센터) , 이상현 (중앙대학교 식물생명공학과) , 유광원 (한국교통대학교 식품영양학전공)

Abstract ▼ AI-Helper

To utilize Malus pruniforia Borkh. as a functional material, cold-water (CW), hot-water (HW), and 70% ethanol (EtOH) extracts were prepared, and their antioxidant and anti-inflammatory activities were compared. The antioxidant activity of the HW extract evaluated by ABTS and DPPH radical scavenging and FRAP activity was significantly effective. The total polyphenol content of the HW extract was also higher by 15.5±0.7 mg GAE/g extract compared to other extracts. The EtOH extract showed significantly decreased TNF-α (39.8%), IL-6 (65.5%), and NO (34.9%) levels in RAW 264.7 cells compared to the LPS-induced control group. The levels of IL-6 (21.1%) and IL-8 (19.3%) were significantly decreased by treatment of EtOH extract in HaCaT keratinocytes induced with TNF-α and IFN-γ. The UHPLC-MS results indicated that the EtOH extract might have chlorogenic acid and phlorizin as the major compounds. This was validated using HPLC-DAD, which showed that the EtOH extract had higher levels of chlorogenic acid and phlorizin (1,185±58 and 470±10 ㎍/g extract, respectively). In conclusion, the present study suggested that the anti-inflammatory activity of the EtOH extract was more effective than the CW and HW extracts, and chlorogenic acid and phlorizin could be used as indicator compounds and functional substances.

주제어

표/그림 (7)

표 Table 1. Antioxidant capacities and total polyphenol of solvent extracts from Malus prunifolia Borkh
그림 Fig. 1. Cytotoxic effect of solvent extracts prepared from Malus prunifolia Borkh. Using (A) RAW 264.7 macrophage and (B) HaCaT keratinocyte. Each sample were treated to RAW 264.7 or HaCaT cells, and inflammation inducer, such as lipopolysaccharide (LPS) or TNF-α and IFN-γ (T+I) were subsequently stimulated for 24 hr. A dexamethasone (50 and 20 μg/mL, for RAW 264.7 and HaCaT, respectively) was used as positive controls. Results are expressed as mean±S.D. of three independent test in triplicate. Asterisks mean significant difference between inflammation group (LPS or T+I) and each group by Student's t-test. *p<0.05, **p<0.01, ***p<0.001.
그림 Fig. 2. Anti-inflammatory activities of solvent extracts prepared from Malus prunifolia Borkh. RAW 264.7 cells were treated with sample followed by LPS treatment for 24hr, and production of (A) TNF-α, (B) IL-6 and (C) nitric oxide (NO) were estimated. A dexamethasone (50 μg/mL) or N-nitroarginine methyl ester (L-NAME; 50 μg/mL) were used as positive control (PC) for TNF-α and IL-6, or NO experiments, respectively. Results are expressed as mean±S.D. of three independent test in triplicate. Asterisks mean significant difference between LPS group and each group by Student's t-test. *p<0.05, **p<0.01, ***p<0.001.
그림 Fig. 3. Anti-inflammatory activities on the skin of solvent extracts prepared from Malus prunifolia Borkh. HaCaT keratinocyte were treated with each sample followed by TNF-α+IFN-γ (T+I) treatment for 24hr, and the production of (A) IL-6 and (B) IL-8 were estimated. A dexamethasone (20 μg/mL) were used as positive control (PC). Results are expressed as mean±S.D. of three independent test in triplicate. Results are expressed as mean±S.D. of three independent test in triplicate. Asterisks mean significant difference between T+I group and each group by Student’s t-test. *p<0.05, **p<0.01, ***p<0.001.
그림 Fig. 4. UHPLC chromatogram of EtOH extract prepared from Malus prunifolia Borkh. (A) The PDA chromatogram and MS spectra of the peaks on the (B) 6.37min and (C) 10.42min.
그림 Fig. 5. HPLC chromatogram of (A) chlorogenic acid and (B) phlorizin references, and (C) EtOH extract prepared from Malus prunifolia Borkh.
표 Table 2. Determination of major compounds from solvent extracts from Malus prunifolia Borkh

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