[논문]생약복합물 용매추출물과 조다당획분의 생리활성

신현영; 김훈; 신지영; 이수정; 유광원

doi:10.9799/ksfan.2021.34.1.036

생약복합물 용매추출물과 조다당획분의 생리활성
The Physiological Activity of Crude Polysaccharide Solvent Extracted from Herbal Medicine Mixture 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.34 no.1, 2021년, pp.36 - 46

신현영 (고려대학교 의생명융합과학과) , 김훈 (경희대학교 피부생명공학센터) , 신지영 ((주)코시스바이오 연구소) , 이수정 (경기대학교 식품생물공학전공) , 유광원 (한국교통대학교 식품영양학전공)

Abstract ▼ AI-Helper

After ethanol (BM-E and RW-E) and hot-water (BM-HW and RW-HW) extracts were fractionated from two herbal mixtures (BM and RW), their physiological activities were investigated. All extracts consisted of more than 50% of neutral sugar, with their total polyphenol levels higher than flavonoid levels. Radical scavenging activities of EtOH extracts remained significantly higher compared to that of hot-water extracts, and in particular, RW-E showed consistently higher antioxidant activity than BM-E. When anti-inflammatory activities of the extracts were evaluated by LPS-stimulated RAW 264.7 cells at 10~500 μg/mL non-cytotoxicity doses, BM-E showed significantly higher levels of TNF-α, IL-1β, IL-6, and nitric oxide inhibitory activity than those of hot-water extracts and RW-E. Murine peritoneal macrophage cells were shown to be enhanced in crude polysaccharides (BM-CP and RW-CP fractionated from BM-HW and RW-HW) compared to hot-water extracts and polysaccharide K (PSK, positive control). Especially, RW-CP exhibited higher activity than BM-CP, and component sugar analysis showed that BM-CP mainly contained galacturonic acid, glucose, arabinose, galactose, and xylose (34.5%, 33.9%, 16.1%, 7.1%, and 6.3%, respectively), whereas RW-CP showed different measurements (29.5%, 59.2%, 5.0%, 4.5%, and 0.2%). In conclusion, two herbal mixtures could contain varying sets of physiological activities dependent on different extraction and fractionation methods.

주제어

표/그림 (7)

표 Table 1. Composition of individual herbs for preparing herbal medicine mixture (BM and RW)
표 Table 2. Yield and chemical component of solvent extract
그림 Fig. 1. Anti-oxidative activity on radical scavenging assay. The difference between lower (ABTS) and upper (DPPH) case letters on the bar means that there is a significant difference (p<0.05) among tested groups.
그림 Fig. 2. Anti-inflammatory activity of ethanol and hot-water extracts in LPS-stimulated RAW 264.7 cells. (A) RAW 264.7 cell viability and inhibitory activity of (B) IL-1β, (C) IL-6, (D) TNF-α, and (E) nitric oxide were estimated by WST and ELISA methods. NC means negative control treated with only saline. LPS means inflammation-induced control treated with only LPS (1 μg/mL). Different asterisks on the column indicated significant difference between LPS and each sample by Student's t-test. *p<0.05, **p<0.01, ***p<0.001.
그림 Fig. 3. Effect of ethanol and hot-water extracts on the production of IL-6 (A), IL-12 (B), and TNF-α (C) in murine peritoneal macrophages. Saline and LPS (1 μg/mL) were used as negative and positive control, respectively. Different asterisks on the column indicate significant difference between saline and each group by Student’s t-test. *p<0.05, **p<0.01, ***p<0.001.
그림 Fig. 4. Effect of ethanol and hot-water extracts on the production of IL-6 (A), IL-12 (B), and TNF-α (C) in murine peritoneal macrophage. Saline was used as negative control, whereas PSK (polysaccharide K) and LPS (1 μg/mL) were used as positive control, respectively. Different asterisks on the column indicated significant difference between saline and each sample by Student's t-test. *p<0.05, **p<0.01, ***p<0.001.
표 Table 3. Component sugar of crude polysaccharide on HPLC analysis

참고문헌 (34)

Alazawi W, Pimadjid N, Lahiri R, Bhattacharya S. 2016. Inflammatory and immune responses to surgery and their clinical impact. Ann Surg 264:73-80

상세보기
Blumenkrantz N, Asboe-Hansen G. 1973. New method for quantitative determination of uronic acids. Anal Biochem 54:484-489

상세보기
Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254

상세보기
Burton RA, Gidley MJ, Fincher GB. 2010. Heterogeneity in the chemistry, structure and function of plant cell walls. Nat Chem Biol 6:724-732

상세보기
Cao Y, Zhao D, Xu AT, Shen J, Ran ZH. 2015. Effects of immunosuppressants on immune response to vaccine in inflammatory bowel disease. Clin Med J 128:835-838
Choi Y, Lee SM, Chun J, Lee HB, Lee J. 2006. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chem 99:381-387

상세보기
Conrad RE. 1981. Induction and collection of peritoneal exudates macrophages. In Herscowitz BH, Holden HT, Ballanti JA, Ghaffar A (Eds.), Manual of Macrophage Methodology. pp.5-11. Marcel Dekker
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal Chem 28:350-356

상세보기
Goupy P, Dufour C, Loonis M, Dangles O. 2003. Quantitative kinetic analysis of hydrogen transfer reactions from dietary polyphenols to the DPPH radical. J Agric Food Chem 51:615-622

상세보기
Han SH, Lim JK. 2001. Effect of Cnidium officinale Makino aqua-acupuncture solution (COMAS) and Cnidium officinale Makino water-extracted solution (COMWS) on the induction of phase II detoxification enzymes. J Korean AM-Meridian Pointol Soc 18:13-18
Honda S, Akao E, Suzuki S, Okuda M, Kakehi K, Nakamura J. 1989. High-performance liquid chromatography of reducing carbohydrates as strongly ultraviolet-absorbing and electrochemically sensitive 1-phenyl-3-methyl5-pyrazolone derivatives. Anal Biochem 180:351-357

상세보기
Hsieh YHP, Ofori JA. 2007. Innovations in food technology for health. Asia Pac J Clin Nutr 1:65-73
Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura Y, Ueno K. 1996. A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. Biol Pharm Bull 19:1518-1520

상세보기
Jackman C, Tomay F, Duong L, Abdol Razak NB, Pixley FJ, Metharom P, Nelson DJ. 2017. Aging and cancer: The role of macrophages and neutrophilis. Ageing Res Rev 36: 105-116

상세보기
Kang MJ, Shin JH, Lee SJ, Chung MJ, Sung NJ. 2009. Effect of garlic and medicinal plants composites on the liver function and lipid metabolism of rats administered with ethanol during the short-term. J Life Sci 7:934-942
Kim D, Ahn M, Jung J, Kwon S, Park EJ, Koo KH, Woo JM. 2015. Perspectives on the market globalization of Korean herbal manufacturers: A company-based survey. Evid Based Complementary Alternat Med 2015:515328
Kim H, Hong HD, Shin KS. 2017. Structure elucidation of an immunostimulatory arabinoxylan-type polysaccharide prepared from young barley leaves (Hordeum vulgare L.). Carbohydr Polym 157:282-293

상세보기
Kim H, Kwak BS, Hong HD, Suh HJ, Shin KS. 2016. Structural features of immunostimulatory polysaccharide purified from pectinase hydrolysate of barley leaf. Int J Biol Macromol 87:308-316

상세보기
Kim H, Park CK, Jeong JH, Jeong HS, Lee HY, Yu KW. 2010. Immune stimulation and anti-metastasis of crude polysaccharide from submerged culture of Hericium erinaceum in the medium supplemented with Korean ginseng extracts. J Korean Soc Food Sci Ntur 38:1535-1542
Kim SN, Li YC, Xu HD, Yi DG, Kim MS, Lee SP, Yi KT, Lee JK, Kim SJ, Kwon MS, Chang PS, Kwak BY. 2008. Phytoestrogenic effects of combined plant extracts on the change of bone metabolism of OVX rats. Korean J Food Sci Technol 40:316-320
Kim YE, Lee YC, Kim HK, Kim CJ. 1997. Antioxidative effect of ethanol fraction for several Korean medicinal plant hot water extracts. Korean J Food Nutr 10:141-144
Korea Health Supplements Association. 2016. Approved Functional Ingredients for Health Functional Foods in Korea (Individually Recognized by the Government). Available from http://khsa.or.kr/user/eng/Khsa.do?_menu103 [cited 1 December 2020]
Lee KH, Lee DJ, Kim SM, Je SH, Kim EK, Han HS, Han IK. 2005. Evaluation of effectiveness and safety of natural plant extract (estromon) on perimenopausal women for 1 year. J Korean Soc Menopause 11:16-26
Lee KH, Rhee KH. 2015. Screening of anti-inflammatory herbs having the activation of MAPK family proteins. Korean J Food Nutr 28:343-350

원문보기 상세보기
Lee SG, Jeong HJ, Lee EJ, Kim JB, Choi SW. 2011. Antioxidant and anti-inflammatory activities of ethanol extracts from medicinal herb mixtures. Korean J Food Sci Technol 43:200-205

원문보기 상세보기
Marinova D, Ribarova F, Atanassova M. 2005. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. J Univ Chem Technol Metall 40:255-260
Park S, Yang MJ, Ha SN, Lee JS. 2014. Effective anti-aging strategies in an era of super-aging. J Menopausal Med 20:85-89

상세보기
Rivera G, Bocanegra-Garcia V, Monge A. 2010. Traditional plants as source of functional foods: A review. CyTA J Food 8:159-167

상세보기
Ryu JY, Park JY, Sung AD, Sung SH, 2020. The utilization of traditional herbal medicine for treatment in traditional Korean medicine clinics. In Hassan B (Ed.), Medicinal Plants: Use in Prevention and Treatment Diseases. pp.82-92. IntechOpen
Stankovic MS. 2011. Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac J Sci 33:63-72
Suh MG, Choi HS, Cho K, Park SS, Kim WJ, Suh HJ, Kim H. 2020. Anti-inflammatory action of herbal medicine comprised of Scutellaria baicalensis and Chrysanthemum morifolium. Biosci Biotechnol Biochem 84:1799-1809

상세보기
Tursun X, Zhao Y, Talat Z, Xin X, Tursun A, Abdulla R, AkberAisa H. 2016. Anti-inflammatory effect of Rosa rugosa flower extract in lipopolysaccharide-stimulated RAW 264.7 macrophages. Biomol Ther 24:184-190

원문보기 상세보기
Tzianabos O. 2000. Polysaccharide immunomodulators as therapeutic agents: Structural aspects and biologic function. Clin Microbiol Rev 13:523-533

상세보기
Yang Y, Zhang Z, Li S, Ye X, Li X, He K. 2014. Synergy effects of herb extracts: Pharmacokinetics and pharmacodynamic basis. Fitoterapia 92:133-147

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증