[논문]상황버섯(Phellinus linteus) 균사체 액체발효물의 항염증 활성

신현영; 김훈; 정은진; 김현경; 손승우; 서민근; 김나리; 서형주; 유광원

doi:10.9799/ksfan.2021.34.5.487

상황버섯(Phellinus linteus) 균사체 액체발효물의 항염증 활성
Anti-Inflammatory Activity of Liquid Fermentation by Phellinus linteus Mycelium 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.34 no.5, 2021년, pp.487 - 497

신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공) , 김훈 (중앙대학교 생명공학대학) , 정은진 (한국교통대학교 식품영양학전공) , 김현경 (한국교통대학교 식품영양학전공) , 손승우 (고려대학교 대학원 의생명융합과학과) , 서민근 ((주)네오크레마 연구개발부) , 김나리 ((주)네오크레마 연구개발부) , 서형주 (고려대학교 대학원 의생명융합과학과) , 유광원 (한국교통대학교 식품영양학전공)

Abstract ▼ AI-Helper

To investigate the industrial availability of liquid fermentation (PL-ferment) by Phellinus linteus mycelium as a postbiotics for the inhibition of inflammation, PL-ferment was fractionated into culture supernatant (CS), hot-water extract (HW) from PL-ferment, EtOH-precipitate (CP) fractionated from HW, and the dialysate (DCP) of CP. Compared to the other fractions, DCP which is expected to contain exopolysaccharide (EPS) as the major component, significantly decreased the production of NO, IL-6, and MCP-1 in LPS-induced RAW 264.7 cells, and IL-6 and IL-8 in TNF-α and IFN-γ-induced HaCaT cells. The general component analysis results showed that no significant difference in components was observed between the fractions, whereas sugar composition analysis revealed that DCP had decreased glucose and increased mannose contents compared to the other fractions. This suggests that mannose played an important role in the anti-inflammatory activity of the active fraction, DCP. Molecular weight distribution analysis revealed that DCP was mainly composed of low-molecular-weight material-removed high-molecular-weight polysaccharides of 18-638 kDa, suggesting that EPS originated from P. linteus EPS. In conclusion, our results suggest that the DCP of P. linteus mycelium fermentation using the anti-inflammatory activity could be used industrially as postbiotic material.

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표/그림 (6)

$Table 1. Analytical condition of HPLC applied for determining the component sugar and molecular weight of fractions from PL-ferment$ 표 Table 1. Analytical condition of HPLC applied for determining the component sugar and molecular weight of fractions from PL-ferment
$Fig. 1. Cytotoxic effect of fractions from PL-ferment using (A) RAW 264.7 macrophage and (B) HaCaT keratinocyte. Each sample were treated to RAW 264.7 or HaCaT cells, and inflammation inducer, such as lipopolysaccharide (LPS) or TNF-α and IFN-γ (T+I) were subsequently stimulated for 24 hr. A dexamethasone (50 and 20 μg/mL, for RAW 264.7 and HaCaT, respectively) was used as positive controls. Results are expressed as mean±S.D. of three independent test in triplicate. Asterisks mean significant difference between inflammation group (LPS or T+I) and each group by Student’s t-test. *p<0.05, **p<0.01, ***p<0.001.$ 그림 Fig. 1. Cytotoxic effect of fractions from PL-ferment using (A) RAW 264.7 macrophage and (B) HaCaT keratinocyte. Each sample were treated to RAW 264.7 or HaCaT cells, and inflammation inducer, such as lipopolysaccharide (LPS) or TNF-α and IFN-γ (T+I) were subsequently stimulated for 24 hr. A dexamethasone (50 and 20 μg/mL, for RAW 264.7 and HaCaT, respectively) was used as positive controls. Results are expressed as mean±S.D. of three independent test in triplicate. Asterisks mean significant difference between inflammation group (LPS or T+I) and each group by Student’s t-test. *p<0.05, **p<0.01, ***p<0.001.
$Fig. 2. Anti-inflammatory activities of fractions from PL-ferment on LPS-stimulated RAW 264.7 cell line. RAW 264.7 cells were treated with sample followed by LPS treatment for 24 hr, and production of (A) nitric oxide (NO), (B) IL-6 and (C) MCP-1 were estimated. A dexamethasone (50 μg/mL) or N-nitroarginine methyl ester (L-NAME; 50 μg/mL) were used as positive control (PC) for TNF-α and IL-6, or NO experiments, respectively. Results are expressed as mean±S.D. of three independent test in triplicate. Asterisks mean significant difference between LPS group and each group by Student's t-test. *p<0.05, **p<0.01, ***p<0.001.$ 그림 Fig. 2. Anti-inflammatory activities of fractions from PL-ferment on LPS-stimulated RAW 264.7 cell line. RAW 264.7 cells were treated with sample followed by LPS treatment for 24 hr, and production of (A) nitric oxide (NO), (B) IL-6 and (C) MCP-1 were estimated. A dexamethasone (50 μg/mL) or N-nitroarginine methyl ester (L-NAME; 50 μg/mL) were used as positive control (PC) for TNF-α and IL-6, or NO experiments, respectively. Results are expressed as mean±S.D. of three independent test in triplicate. Asterisks mean significant difference between LPS group and each group by Student's t-test. *p<0.05, **p<0.01, ***p<0.001.
$Fig. 3. Anti-inflammatory activities of fractions from PL-ferment on TNF-α+IFN-γ (T+I) -stimulated HaCaT keratinocyte. HaCaT keratinocyte were treated with each sample followed by TNF-α+IFN-γ (T+I) treatment for 24 hr, and the production of (A) IL-6 and (B) IL-8 were estimated. A dexamethasone (20 μg/mL) were used as positive control (PC). Results are expressed as mean±S.D. of three independent test in triplicate. Asterisks mean significant difference between T+I group and each group by Student's t-test. *p<0.05, **p<0.01, ***p<0.001.$ 그림 Fig. 3. Anti-inflammatory activities of fractions from PL-ferment on TNF-α+IFN-γ (T+I) -stimulated HaCaT keratinocyte. HaCaT keratinocyte were treated with each sample followed by TNF-α+IFN-γ (T+I) treatment for 24 hr, and the production of (A) IL-6 and (B) IL-8 were estimated. A dexamethasone (20 μg/mL) were used as positive control (PC). Results are expressed as mean±S.D. of three independent test in triplicate. Asterisks mean significant difference between T+I group and each group by Student's t-test. *p<0.05, **p<0.01, ***p<0.001.
그림 Fig. 4. Molecular weight chromatogram of (A) CP and (B) DCP from PL-ferment.
$Table 2. Chemical properties of fractions from PL-ferment$ 표 Table 2. Chemical properties of fractions from PL-ferment

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