[논문]산화철 나노입자를 함유한 초상자성 마이크로니들에 관한 연구

이승준

doi:10.14478/ace.2021.1071

산화철 나노입자를 함유한 초상자성 마이크로니들에 관한 연구
Study of Superparamagnetic Microneedles containing Iron Oxide Nanoparticles 원문보기

공업화학 = Applied chemistry for engineering, v.32 no.5, 2021년, pp.556 - 561

이승준 (서원대학교 제약공학과 공정개발연구실)

초록
AI-Helper

최근 산화철 나노입자는 외부의 자기장에 반응하는 자성의 특성과 생체적합성이 뛰어나 약물전달시스템(drug delivery system, DDS)에 관한 많은 연구의 소재로 사용되어져 왔다. 본 연구에서는 마이크로니들(microneedles, MNs)의 매트릭스 물질로 HA (hyaluronic acid)와 CMC (carboxy methyl cellulose)를 이용하여 SIONs (superparamagnetic iron oxide nanoparticles)이 함유된 HA-SMNs (hyaluronic acid-superparamagnetic microneedles)와 CMC-SMNs (carboxy methyl cellulose-superparamagnetic microneedles)를 제조하였으며, SEM (scanning electron microscopic), SQUD-VSM (superconducting quantum interference device-vibrating sample magnetometer), FMMD (frequency mixing magnetic detection), 고분자 및 바이오 멤브레인을 이용하여 SMNs의 다양한 특성을 조사하였다. SQUID-VSM 측정 결과 SIONs이 포함된 HA-SMNs와 CMC-SMNs에서 초상자성의 특성이 나타났으며, FMMD 측정에서는 SIONs 농도가 증가함에 따라 신호 강도에 변화가 확인되었다. 또한 SMNs의 바이오 막을 통한 HA-SMNs와 CMC-SMNs의 투과도 분석에서는 각각 평균 92.5%와 98.5%의 피부 투과율이 조사되었다. 이러한 결과를 통해 SMNs 제형은 경피약물전달시스템(transdermal drug delivery system, TDDS) 및 MR(magnetic mesonance) molecular imaging 분야의 전달소재로 활용될 수 있을 것으로 기대한다.

Abstract ▼ AI-Helper

Recently, iron oxide nanoparticles have been used as the subject of many studies on drug delivery system (DDS) due to their excellent magnetic properties and biocompatibility in response to external magnetic fields. In this study, hyaluronic acid-superparamagnetic microneedles (HA-SMNs) and carboxy methyl cellulose-superparamagnetic microneedles (CMC-SMNs) containing superparamagnetic iron oxide nanoparticles (SIONs) were prepared with HA and CMC as a matrix materials of MNs (microneedles). Various properties of SMNs were then investigated with scanning electron microscopy (SEM), superconducting quantum interference device-vibrating sample magnetometer (SQUD-VSM), frequency mixing magnetic detection (FMMD), and polymer/bio membrane. The SQUID-VSM measurements showed superparamagnetism of HA-SMNs and CMC-SMNs containing SIONs. The FMMD results demonstrated that the signal intensity changed significantly as the concentration of SIONs increased. In addition, SMNs exhibited the average skin permeability intensities on the bio membrane for HA-SMNs and CMC-SMNs were 92.5 and 98.5%, respectively. These results suggested that SMNs could be utilized as deliver materials for a TDDS and MR molecular imaging.

주제어

표/그림 (8)

그림 Figure 1. The schematic diagram and Image of Microneedles (MNs) Master.
그림 Figure 3. SEM images of HA-SMNs and CMC-SMNs Morphologies (scale bar 500 um).
그림 Figure 2. (a) Process flow for the fabrication of HA-SMNs and CMC-SMNs containing SIONs. (b) Image of HA-MNs not containing SIONs. (c) Image of HA-SMNs containing SIONs.
그림 Figure 4. The magnetic susceptibility of SIONs (a) and HA/CMC-SMNs containing SIONs 0.25%, 0.5%, 1% (b).
그림 Figure 5. The magnetic property(a) and the signal intensity(b) of HA-SMNs and CMC-SMNs containing SIONs 0.25%, 0.5%, 1%.
그림 Figure 6. Optical Microscope images of permeability on the polymer membrane permeability with HA-SMNs and CMC-SMNs containing SIONs 0%, 0.25%, 0.5%, 1%.
그림 Figure 7. Optical Microscope images of permeability on the bio membrane(porcine back skin) permeability with HA-SMNs and CMC-SMNs containing SIONs 0%, 0.25%, 0.5%, 1%.
표 Table 1. The Permeability Intensity of the Polymer Membrane and the Bio Membrane with HA-SMNs and CMC-SMNs containing SIONs 0%, 0.25%, 0.5%, 1%

참고문헌 (27)

J. S. Lee, J. H. Lee, J. K. Shim, and W. Hur, Microencapsulation of Iron Oxide Nanoparticles and Their Application in Magnetic Levitation of Cells, Appl. Chem. Eng., 31(1), 13-18 (2020).
T. Neuberger, B. Schopf, H. Hofmann, M. Hofmann, and B. Von Rechenberg, Superparamagnetic nanoparticles for biomedical applications: Possibilities and limitations of a new drug delivery system, J. Magn. Magn. Mater., 293, 483-496 (2005).

상세보기
K. Mahmoudi, A. Bouras, D. Bozec, R. Ivkov, and C. Hadjipanayis, Magnetic hyperthermia therapy for the treatment of glioblastoma: A review of the therapy's history, efficacy and application in humans, Int. J. Hyperthermia, 34, 1316-1328 (2018).

상세보기
H. K. Min, J. A. Kim, J. C. Kim, and S. J. Lee, Preparation and Characterization of Superparamagnetic Microneedles (SMMNs) containing Magnetic Cubosome Nanoparticles (MCNs), J. Adv. Eng. Technol., 12(3), 103-108 (2019).

상세보기
S. J. Lee, J. R. Jeong, S. C. Shin, and J. D. Kim, Synthesis and Characterization of Superparamagnetic Maghemite Nanoparticles Prepared by Coprecipitation Technique, J. Magn. Magn. Mater., 282, 147-150 (2004).

상세보기
S. J. Lee, J. R. Jeong, S. C. Shin, Y. M. Huh, H. T. Song, J. S. Suh, Y. H. Chang, B. S. Jeon, and J. D. Kim, Intracellular translocation of superparamagnetic iron oxide nanoparticles encapsulated with peptide-conjugated Poly(D,L lactide-co-glycolide), J. Appl. Phys., 97, 10Q913 (2005).

상세보기
S. J. Lee, J. R. Jeong, S. C. Shin, Y. H. Chang, and J. D. Kim, Magnetic Enhancement of Iron Oxide Nanoparticles Encapsulated with Poly(D,L latide-co-glycolide), Colloids Surf. A, 225, 19-25 (2005).
J. R. Jeong, S. J. Lee, J. D. Kim, and S. C. Shin, Magnetic Properties of Fe3O4 Nanoparticle Encapsulated with Poly(D,L Laticde-co-Glycolide), IEEE Trans. Mag., 40(4), 3015-3017 (2004).

상세보기
D. Ling, N. Lee, and T. Hyeon, Chemical synthesis and assembly of uniformly sized iron oxide nanoparticles for medical applications, Acc. Chem. Res., 48, 1276-1285 (2015).

상세보기
R. Wassel, B. Grady, R. Kopke, and K. Dormer, Dispersion of superparamagnetic iron oxide nanoparticles in poly (D,L-lactide-coglycolide) microparticles, Colloids Surf. A, 292, 125-130 (2007).

상세보기
S. J. Lee, H. J. Kim, Y. M. Huh, I. W. Kim, J. H. Jeong, J. C. Kim, and J. D. Kim, Functionalized magnetic PLGA Nanospheres for Targeting and Bioimaging of breast cancer, J. Nanosci. Nanotechnol., 18, 1542-1547 (2018)

상세보기
H. Ryu, H. Koo, I. Sun, S. Yuk, K. Choi, K. Kim, and I. Kwon, Tumor-targeting multi-functional nanoparticles for theragnosis: New paradigm for cancer therapy, Adv. Drug Deliv. Rev., 64, 1447-1458 (2012).

상세보기
H. Na, I. Song, and T. Hyeon, Inorganic nanoparticles for MRI contrast agents, Adv. Mater., 21, 2133-2148 (2009).

상세보기
Y. G. Kim, S. M. Lee, D. B. Kim, K. M. Noh, K. S. Oh, S. H. Cho, E. P. Choi, K. P. Kim, and K. M. Huh, Preparation and Characterization of Poly(ethylene glycol)-Doxorubicin/SPION Magnetic Nanoparticles for Cancer Therapy, Polymer(Korea), 42(6) 1059-1067 (2018).

상세보기
J. H. Park, S. O. Choi, S. Seo, Y. B. Choy, and M. R. Prausnitz, A microneedle roller for transdermal drug delivery, Eur. J. Pharm. Biopharm., 76, 282 (2010).

상세보기
J. W. Lee, S. O. Choi, E. I. Felner, and M. R. Prausnitz, Dissolving Microneedle Patch for Transdermal Delivery of Human Growth Hormone, Small, 7(4), 531 (2011)

상세보기
S. K. Han, H. Y. Ha, and S. J. Lee, Fabrication of Microneedles array Combining Biodegradable Hyaluronic acid and Herbal Medicine Lonicera flos, Polymer(Korea), 43(4), 540-546 (2019).
S. K. Han, S. J. Lee, and H. Y. Ha, Skin Moisturizing Effects of a Microneedle Patch Containing Hyaluronic Acid and Lonicerae flos, Processes, 9, 321, 1-8 (2021).

상세보기
G. Bonfante, H. J. Lee, L. Bao, J. H. Park, N. Takama, and B. J. Kim, Comparison of polymers to enhance mechanical properties of microneedles for bio-medical applications, Micro Nano Syst. Lett. 8:13 (2020).

상세보기
R. V. Dixon, E. Skaria, W. M. Lau, P. Manning, M. A. Birch-Machin, S. M. Moghimi, and K. W. Ng, Microneedle-based devices for point-of-care infectious disease diagnostics, Acta Pharm. Sin. B., 11(8), 2344-2361 (2021).

상세보기
Z. Wang, J. Luan, A. Seth, L. Liu, M. You, P. Gupta, P. Rathi, Y. Wang, S. Cao, Q. Jiang, X. Zhang, R. Gupta, Q. Zhou, J. J. Morrissey, E. L. Scheller, J. S. Rudra, and S. Singamaneni, Microneedle patch for the ultrasensitive quantification of protein biomarkers in interstitial fluid, Nat. Biomed. Eng., 5, 64-76 (2021).

상세보기
S. J. Lee, Fabrication and Characterization of Micropatterned Elastomeric PDMS Mold, J. Adv. Eng. Technol., 20(3), 335 (2017).
C. B. Kim, E. G. Lim, S. W. Shin, H. J. Krause, and H. B. Hong, Magnetic immunoassay platform based on the planar frequency mixing magnetic technique, Biosens. Bioelectron., 83(15), 293-299 (2016).

상세보기
H. B. Hong, H. J. Krause, I. H. Nam, C. J. Choi, and S. W. Shin, Magnetic immunoassay based on frequency mixing magnetic detection and magnetic particles of different magnetic properties, Anal. Methods, 6(19), 8055-8058 (2014).

상세보기
H. J. Krause, N. Wolters, Y. Zhang, A. Offenhausser, P. Miethe, M. Meyer, M. Hartmann, and M. Keusgen, Magnetic particle detection by frequency mixing for immunoassay applications, J. of J. Magn. Magn. Mater., 311, 436-444 (2007).

상세보기
S. J. Lee, J. R. Jeong, S. C. Shin, J. C. Kim, Y. H. Chang, Y. M. Chang, and J. D. Kim, Nanoparticles of magnetic ferric oxides encapsulated with poly (D, L latide-co-glycolide) and their applications to magnetic resonance imaging contrast agent, J. Magn. Magn. MAter., 272-276, 2432-2433 (2004).

상세보기
H. Li, S. Yang, D. Hui, and R. Hong, Progress in magnetic Fe3O4 nanomaterials in magnetic resonance imaging, Nanotechnol. Rev., 9, 1265-1283 (2020).

상세보기

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