[논문]염생식물 도깨비고비의 항산화 및 항염증 효과

김현모; 김호준; 공창숙; 이봉호; 심현보; 서영완

doi:10.4217/opr.2021.43.3.113

염생식물 도깨비고비의 항산화 및 항염증 효과
Antioxidant and Anti-inflammatory Activities of the Halophyte Cyrtomium falcatum 원문보기

Ocean and polar research, v.43 no.3, 2021년, pp.113 - 126

김현모 (한국해양대학교 해양과학융합학부) , 김호준 (한국해양바이오클러스터) , 공창숙 (신라대학교 의생명과학대학 식품영양학과) , 이봉호 (한밭대학교 공과대학 화학생명공학과) , 심현보 (인천과학예술영재학교) , 서영완 (한국해양대학교 해양과학융합학부)

Abstract ▼ AI-Helper

In the present study, the halophyte C. falcatum extract and its solvent fractions (n-hexane, 85% aqueous methanol, n-butanol, and water) were evaluated for antioxidant and anti-inflammatory activities. Antioxidative ability was measured by DPPH radical, intracellular reactive oxygen species (ROS) and peroxynitrite scavenging, DNA oxidation inhibition, and ferric reducing antioxidant power (FRAP). For DPPH radical and peroxynitrite scavenging, DNA oxidation inhibition, and FRAP, 85% aq.MeOH and n-BuOH fractions showed significant scavenging activity. For production of intracellular ROS in HT-1080 cells, 85% aq.MeOH fraction showed the highest scavenging activity. In addition, anti-inflammatory activity was also assessed by measuring the inhibitory effect against mRNA expression of pro-inflammatory factors (NO, IL-1β, IL-6 and COX-2) in LPS-stimulated Raw 264.7 macrophages. For NO production, crude extract exhibited a strong inhibitory effect at a concentration of 100 ㎍/ml. For mRNA expression of pro-inflammatory cytokines (IL-1β, IL-6, and COX-2), n-BuOH greatly suppressed expression levels of IL-1β and IL-6 at 100 ㎍/ml concentration while 85% aq. MeOH fraction significantly inhibited that of COX-2 even at 100 ㎍/ml. These results suggest that C. falcatum may be used as a potential source for the development of a natural antioxidant or anti-inflammatory agent.

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표/그림 (14)

$Fig. 1. Procedure for obtaining crude extract and its solvent fractions from Cyrtomium falcatum$ 그림 Fig. 1. Procedure for obtaining crude extract and its solvent fractions from Cyrtomium falcatum
$Fig. 2. DPPH radical scavenging activity of crude extract and its solvent fractions of C. falcatum. 85% aq.MeOH: 85% aqueous methanol, n-BuOH: n-butanol, BHA: butylated hydroxyanisole. a–kMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan’s multiple range test$ 그림 Fig. 2. DPPH radical scavenging activity of crude extract and its solvent fractions of C. falcatum. 85% aq.MeOH: 85% aqueous methanol, n-BuOH: n-butanol, BHA: butylated hydroxyanisole. ^a–kMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan’s multiple range test
$Fig. 4. Ferric reducing antioxidant power (FRAP) of crude extract and its solvent fractions of C. falcatum. a–lMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test$ 그림 Fig. 4. Ferric reducing antioxidant power (FRAP) of crude extract and its solvent fractions of C. falcatum. ^a–lMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test
$Fig. 3. Scavenging activity of crude extract and its solvent fractions of C. falcatum on authentic ONOO- (a) and ONOO- from SIN-1 (b). a–hMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan’s multiple range test$ 그림 Fig. 3. Scavenging activity of crude extract and its solvent fractions of C. falcatum on authentic ONOO^- (a) and ONOO^- from SIN-1 (b). ^a–hMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan’s multiple range test
$Fig. 5. Cell viabillity of crude extract and its solvent fractions of C. falcatum on HT-1080 cells. a–dMeans with differentletters at the same concentration are significantly different (p < 0.05) by Duncan’s multiple range test$ 그림 Fig. 5. Cell viabillity of crude extract and its solvent fractions of C. falcatum on HT-1080 cells. ^a–dMeans with differentletters at the same concentration are significantly different (p < 0.05) by Duncan’s multiple range test
$Fig. 6. ROS scavenging activity of crude extract and its solvent fractions of C. falcatum on intracellular ROS levels induced by H2O2 in HT-1080 cells$ 그림 Fig. 6. ROS scavenging activity of crude extract and its solvent fractions of C. falcatum on intracellular ROS levels induced by H₂O₂ in HT-1080 cells
$Fig. 7. Antioxidant activity of crude extract and its solvent fractions of C. falcatum on genomic DNA in HT-1080 cells. a–cMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test$ 그림 Fig. 7. Antioxidant activity of crude extract and its solvent fractions of C. falcatum on genomic DNA in HT-1080 cells. ^a–cMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test
$Table 1. Total polyphenol and total flavonoid contents of crude extract and its solvent fractions of C. falcatum. a–cMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test$ 표 Table 1. Total polyphenol and total flavonoid contents of crude extract and its solvent fractions of C. falcatum. a–cMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test
$Fig. 8. Cell viabillity of crude extract and its solvent fractions of C. falcatum on Raw 264.7 cells. a–dMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test$ 그림 Fig. 8. Cell viabillity of crude extract and its solvent fractions of C. falcatum on Raw 264.7 cells. ^a–dMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test
$Fig. 9. Inhibition activity of crude extract and its solvent fractions of C. falcatum on NO production in Raw 264.7 cells. a–bMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan’s multiple range test$ 그림 Fig. 9. Inhibition activity of crude extract and its solvent fractions of C. falcatum on NO production in Raw 264.7 cells. ^a–bMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan’s multiple range test
$Fig. 10. Effect of crude extract, and its n-hexane and 85% aq.MeOH fractions of C. falcatum on mRNA levels of IL-1β and IL-6 determined by RT-PCR in Raw 264.7 cells. a-kMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test$ 그림 Fig. 10. Effect of crude extract, and its n-hexane and 85% aq.MeOH fractions of C. falcatum on mRNA levels of IL-1β and IL-6 determined by RT-PCR in Raw 264.7 cells. ^a-kMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test
$Fig. 11. Effect of n-BuOH and water fractions of C. falcatum on mRNA levels of IL-1β and IL-6 determined by RT-PCR in Raw 264.7 cells. a-eMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test$ 그림 Fig. 11. Effect of n-BuOH and water fractions of C. falcatum on mRNA levels of IL-1β and IL-6 determined by RT-PCR in Raw 264.7 cells. ^a-eMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test
$Fig. 12. Effect of crude extract and its n-hexane and 85% aq.MeOH fractions of C. falcatum on mRNA levels of COX-2 determined by RT-PCR in Raw 264.7 cells. a-hMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test$ 그림 Fig. 12. Effect of crude extract and its n-hexane and 85% aq.MeOH fractions of C. falcatum on mRNA levels of COX-2 determined by RT-PCR in Raw 264.7 cells. ^a-hMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test
$Fig. 13. Effect of n-BuOH and water fractions of C. falcatum on mRNA levels of COX-2 determined by RT-PCR in Raw 264.7 cells. a-gMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test$ 그림 Fig. 13. Effect of n-BuOH and water fractions of C. falcatum on mRNA levels of COX-2 determined by RT-PCR in Raw 264.7 cells. ^a-gMeans with different letters at the same concentration are significantly different (p < 0.05) by Duncan's multiple range test

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