[논문]머위(Petasites japonicus)의 피부장벽과 항염증 효과

김채현; 문외숙; 장영아

doi:10.12925/jkocs.2023.40.2.258

머위(Petasites japonicus)의 피부장벽과 항염증 효과
Skin barrier and anti-inflammatory effect of petasites japonicus 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.40 no.2, 2023년, pp.258 - 267

김채현 (경성대학교 화장품학과) , 문외숙 ((주)에코마인) , 장영아 (경성대학교 스마트헬스케어융복합연구센터)

초록
AI-Helper

본 연구에서는 머위에탄올추출물 (PJE)의 기능성 소재로서의 활용 가능성을 알아보기 위해 UVB로 유도된 Human keratinocyte (HaCaT cell)를 통해 피부장벽과 염증 개선에 대한 활성을 알아보고자 하였다. DPPH 라디칼 소거능 측정, ABTS⁺ 라디칼 소거능 측정, Hydrogen peroxide 소거능 측정을 통해 항산화 효과를 확인한 결과, 1 mg/ml 농도에서 대조군인 ascorbic acid과 비슷한 항산화 효과를 가지고 있음을 확인하였다. UVB로 유도된 HaCaT 세포의 filaggrin과 aquaporin-3의 생성능의 mRNA의 발현을 확인해본 결과, UVB 자극에 의해 줄어든 발현량이 머위 추출물 처리 시, 농도 의존적으로 증가하고 있음을 확인하였다. TNF-𝛼와 IL-1𝛽의 mRNA 발현은 UVB 자극에 의해 증가되었으며 머위 추출물을 처리하였을 때 감소되는 것을 확인할 수 있었다. Migration assay 결과 농도 의존적으로 피부 각질세포의 증식과 상처의 회복율을 증가시켰음을 확인하였다. 실험결과를 바탕으로 머위가 피부 보습 및 피부장벽 기능을 개선할 수 있는 기능성 소재의 화장품으로 사용될 수 있음을 시사한다.

Abstract ▼ AI-Helper

In this study, to investigate the possibility of using ethanol extract of Petasites japonicus (PJE) as a functional material, we investigated the activity of improving skin barrier and inflammation through UVB-induced human keratinocyte (HaCaT cell). As a result of confirming the antioxidant effect through DPPH radical scavenging activity, ABTS+ radical scavenging activity, and hydrogen peroxide scavenging activity, it was confirmed that it had an antioxidant effect similar to that of ascorbic acid, a control, at a concentration of 1 mg/ml. As a result of confirming the mRNA expression of the production ability of filaggrin and aquaporin-3 in HaCaT cells induced by UVB, it was confirmed that the reduced expression level by UVB stimulation increased in a concentration-dependent manner when the PJE was treated. It was confirmed that the mRNA expression of TNF-𝛼 and IL-1𝛽 were increased by UVB stimulation and decreased when the PJE was treated. As a result of the migration assay, it was confirmed that the proliferation of skin keratinocytes and the recovery rate of wounds were increased in a concentration-dependent manner. Based on the experimental results, it suggests that Petasites japonicus can be used as a functional cosmetic product that can improve skin moisturizing and skin barrier function.

주제어

표/그림 (8)

표 Table 1. Primer sequences for real-time PCR
그림 Fig. 1. Electron donating ability of Petasites japonicus extracts, ascorbic acid (Positive control), Results are means ± S.D. of triplicate data. Values with different letters^a,b,c,d are significantly different each other by ANOVA and Duncan's multiple range test (p < 0.05).
그림 Fig. 2. ABTS⁺ inhibition activity of Petasites japonicus extracts, ascorbic acid (Positive control), Results are means ± S.D. of triplicate data.Values with different letters^a,b,c,d are significantly different each other by ANOVA and Duncan's multiple range test (p<0.05).
그림 Fig. 3. Hydrogen peroxide scavenging activity of Petasites japonicus extracts, Ascorbic acid (Positive control), Results are means±S.D. of triplicate data. Values with different letters^a,b,c,d are significantly different each other by ANOVA and Duncan's multiple range test (p < 0.05).
그림 Fig. 4. Cell viability assay on human keratinocytes. The cells were treated for 24 h with the indicated concentrations of 0.025, 0.05, 0.1 mg/ml. The data are representative of thee experiments. Each value is expressed as the mean ± SD of thee independent experiments. The statistical analysis of the data was carried out by use of ANOVA test. ^*p < 0.05, compared with absence of extract.
그림 Fig. 5. Expression levels of filaggrin and aquaporin-3 on UVB-irradiated keratinocytes treated with PJE. The cells were treated for 24h with the indicated concentrations of extracts before treatment with UVB (50 mJ/cm²) for 24 h. There sults were analyze dusing ELISA kits. Nor : Neither extract nor UVB treatment, Con : only UVB (50mJ/cm²) treated. The data are representative of three experiments. Each value is expressed as the mean ± SD ofthee independent experiments. The statistical analysis of the data was carried out by use of ANOVA test. ^*p < 0.05, compared with absence of extract.
그림 Fig. 6. TNF-α, IL-1β, filaggrin and aquaporin-3 mRNA expressions on UVB-irradiated human keratinocytes treated with PJE. The cells were treated for 24 h with the indicated concentrations of extracts before treatment with UVB (50 mJ/cm²) for 24 h. There rsults were analyze dusing ELISA kits. Nor : Neither extract nor UVB treatment, Con : only UVB (50mJ/cm²) treated. The data are representative of three experiments. Each value is expressed as the mean ± SD ofthee independent experiments. The statistical analysis of the data was carried out by use of ANOVA test. ^*p < 0.05, compared with absence of extract.
그림 Fig. 7. Effects of a fermented PJE on migration of HaCaT cells. HaCaT cells were treated with PJE at indicated concentration for 24 h. Wound healing assay was performed as described in materials and methods. Phase contrast images were taken at 24 h after scratching to determine healed distance. Values represent mean±S.D. (n=3). ^*p < 0.05, compared with absence of extract.

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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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