[논문]항가려움증 활성에 섬애약쑥(Artemisia argyi H.) 추출물이 미치는 영향

이해진; 임현지; 임미혜

doi:10.12925/jkocs.2021.38.5.1292

항가려움증 활성에 섬애약쑥(Artemisia argyi H.) 추출물이 미치는 영향
Effect of Seomaeyakssuk (Artemisia argyi H.) Extracts on Anti-pruritic Activities 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.38 no.5, 2021년, pp.1292 - 1301

이해진 (대전대학교 뷰티건강관리학과) , 임현지 (대전대학교 미용의학과) , 임미혜 (대전대학교 뷰티건강관리학과)

초록
AI-Helper

본 연구는 섬애약쑥(Artemisia argyi H.)의 항가려움증 활성 가능성을 확인하기 위한 연구로써 가려움증 관련인자 등을 측정하였다. 섬애약쑥은 열수로 추출(Artemisia argyi H. distilled water extract 이하, AAD)하여 MTT assay로 세포독성을 측정하였고 항 가려움증 활성을 확인하기 위하여 IL-4와 IL-31 관련 전사인자 발현 및 단백질 생성을 측정하였으며, 히스타민의 발현을 측정하였다. 그 결과 25, 50, 100 ㎍/㎖의 농도에서는 유의한 세포독성이 나타나지 않는 것을 확인하였다. 가려움증 연관 유전인자인 IL4의 경우 25 ㎍/㎖의 농도에서 약 12%, 50 ㎍/㎖의 농도에서 약 26%, 100 ㎍/㎖의 농도에서 약 61%로 유의하게 감소하였으며, IL31의 경우 50 ㎍/㎖의 농도에서 약 33%, 100 ㎍/㎖의 농도에서 약 33%로 유의하게 감소하였다. 연관된 단백질 측정의 경우 각각 50 ㎍/㎖와 100 ㎍/㎖의 농도에서 IL-4는 약 34% 및 약 69%, IL-31은 약 36% 및 약 37% 유의하게 감소하였다. 이 결과는 ADD가 항가려움증을 위한 소재로써 가능성을 보여 소재 개발을 위한 기초자료로 제공될 수 있을 것으로 사료된다.

Abstract ▼ AI-Helper

The purpose of this study was to investigate the anti-pruritic activities such as cell viability and pruritus-related factor using seomaeyakssuk (Artemisia argyi H.; Seomaeyakssuk) extract on MC/9 (mouse mast cell line). Seomaeyakssuk was extracted from hot distilled water. Cell viability was assessed using MTT assay on MC/9 cells. Anti-pruritic activities were measured through changes in the levels of transcription factor (IL4 and IL31) on MC/9 cells. In addition, the expression of linked proteins and histamine was measured. The results confirmed that significant cytotoxicity does not appear in the concentration range of 25, 50, and 100 ㎍/㎖. The levels of IL4 was reduced to 12% (25 ㎍/㎖), 26% (50 ㎍/㎖) and 61% (100 ㎍/㎖). Also, level of IL31 was decreased 33% (50 ㎍/㎖) and 36% (100 ㎍/㎖). In the case of proteins levels decreased significantly IL-4 34%/69% and IL-31 36%/37% at 50 ㎍/㎖ and 100 ㎍/㎖. Histamine decreased by 22, 58% and 61%, respectively, at concentrations of 25, 50, and 100 ㎍/㎖. This results shows possibility of ADD as raw material in anti-pruritic products.

주제어

표/그림 (5)

그림 Fig. 1. Cell viability of AAD in MC/9 cell. MC/9 cell were treated by 25, 50, 100, and 200 ㎍/㎖ of AAD for 12 h. Treated cells were reacted by EZ-Cytox for 30 min and then absorbance were measured at 450 ㎚ using micro plate reader. Cell viability were calculated as percentage relative to the control. The result were presented by the mean ± S.D from three independent experiments.
표 Table 1. The Sequences of Primers
그림 Fig. 2. Effect of AAD on IL4 mRNA expression level (A) and IL-4 protein level (B) in MC/9 cell. MC/9 cell were treated 25, 50, and 100 ㎍/㎖ of AAD with 1 ㎍/㎖ DNP-IgE for 12 h. The mRNA expression level were measured using a quantitative real-time PCR(qRT-PCR). And, the protein expression level were measured using a western blot. The results were expressed as mean±S.D. (Significance of results, ^***p<0.001, ^**p<0.01, ^*p<0.05 compare to control).
그림 Fig. 3. Effect of AAD on IL31 mRNA expression level (A) and IL-31 protein level (B) in MC/9 cell. MC/9 cell were treated 25, 50, and 100 ㎍/㎖ of AAD with 1 ㎍/㎖ DNP-IgE for 12 h. The mRNA expression level were measured using a quantitative real-time PCR(qRT-PCR). And, the protein expression level were measured using a western blot. The results were expressed as mean±S.D. (Significance of results, ^***p<0.001 compare to control).
그림 Fig. 4. Effect of AAD on histamine protein level in MC/9 cell. MC/9 cell were treated 25, 50, and 100 ㎍/㎖ of AAD with 1 ㎍/㎖ DNP-IgE for 12 h. The protein expression level were measured using a western blot. The results were expressed as mean±S.D. (Significance of results, ^***p<0.001 and ^**p<0.01 compare to control).

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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