[논문]LPS로 유도된 RAW 264.7 세포에서 청폐사간탕(淸肺瀉肝湯)의 항산화 효과

전보희; 김태준; 김희택; 김용민

doi:10.6114/jkood.2021.34.4.024

LPS로 유도된 RAW 264.7 세포에서 청폐사간탕(淸肺瀉肝湯)의 항산화 효과
Anti-oxidative Effect of Chungpyesagan-tang in LPS Induced RAW 264.7 Cells 원문보기

韓方眼耳鼻咽喉皮膚科學會誌 = The journal of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, v.34 no.4, 2021년, pp.24 - 36

전보희 (세명대학교 화장품과학과) , 김태준 (세명대학교 한의과대학 한방안이비인후피부과) , 김희택 (세명대학교 한의과대학 한방안이비인후피부과) , 김용민 (세명대학교 화장품뷰티생명공학부)

Abstract ▼ AI-Helper

Objectives : This study was conducted to confirm the anti-oxidative effect of Chungpyesagan-tang(CPSGT) extract. Methods : In this study, MTT assay was performed to confirm cell viability, and DPPH and ABTS were performed to confirm radical scavenging ability. The ROS scavenging ability and the protective effect against DNA damage were confirmed by 2,7-dichlorofluorescin diacetate(DCF-DA) and 4',6-diamidino-2-phenylindole(DAPI) staining and comet assay. mRNA expression of Heme oxygenase-1(HO-1) was measured by real-time PCR, and expression of HO-1 and Kelch-like ECH-associated protein 1(Keap1) proteins was measured by western blot. Results : CPSGT was not cytotoxic at 50-400㎍/㎖. The radical scavenging activity was increased, and the ROS scavenging activity and the protective effect against DNA damage were increased compared to the LPS-treated group. The mRNA expression and protein expression of HO-1 were increased in a concentration-dependent manner. The protein expression level of Keap1 was decreased in a concentration-dependent manner. Conclusion : This suggests that CPSGT has an antioxidant effect and can be used as a potential material for skin diseases.

주제어

표/그림 (9)

표 Table 1. Contents of CPSGT
표 Table 2. Gene Name and Assay ID Number in Realtime PCR Analysis
그림 Fig. 1. The Effects of CPSGT on Cell Viability in RAW 264.7 Cells Control: untreated group; 25, 50, 100, 200, 400: treated with various concentrations of CPSGT(㎍/㎖)
그림 Fig. 2. The Effects of CPSGT on DPPH and ABTS Radical Scavenging Control: untreated group. Positive control: 200 μM ascorbic acid (AC). 50, 100, 200, 400: treated with various concentrations of CPSGT(㎍/㎖). (A) DPPH radical scavenging, (B) ABTS radical scavenging. (*p<0.05)
그림 Fig. 3. The Effects of CPSGT on Intracellular ROS Generation in LPS-stimulated RAW 264.7 Cells Control: untreated group. LPS: treated with LPS(1㎍/㎖); 50, 100, 200, 400: treated with various concentrations of CPSGT(㎍/㎖) with LPS(1㎍/㎖)
그림 Fig. 4. Inhibition of DNA Damage by CPSGT in LPS-stimulated RAW 264.7 Cells. Control: untreated group. LPS: treated with LPS(1㎍/㎖); 50, 100, 200 400: treated with various concentrations of CPSGT(㎍/㎖) with LPS(1㎍/㎖).
그림 Fig. 5. Inhibition of DNA Damage by CPSGT in LPS-stimulated RAW 264.7 Cells. Control: untreated group. LPS: treated with LPS(1㎍/㎖); 50, 100, 200, 400: treated with various concentrations of CPSGT(㎍/㎖) with LPS(1㎍/㎖)
그림 Fig. 6. The Effects of CPSGT on HO-1 mRNA Expression in LPS-induced RAW 264.7 Cells. Control: untreated group. LPS: treated with LPS(1㎍/㎖); 50, 100, 200, 400: treated with various concentrations of CPSGT(㎍/㎖) with LPS(1㎍/㎖). (*p<0.05)
그림 Fig. 7. The Effects of CPSGT on HO-1 Protein Expressions in LPS-induced RAW 264.7 Cells. Control: untreated group. LPS: treated with LPS(1㎍/㎖); 50, 100, 200, 400: treated with various concentrations of CPSGT(㎍/㎖) with LPS(1㎍/㎖). (A) protein expression level, (B) HO-1, Keap1 protein level was proved by image J. Values are relative to the control. (*p<0.05)

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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