[논문]블루베리와 아로니아를 함유한 청국장 환 제품의 마우스 염증성 장질환 개선 효과

김하림; 노은미; 이승현; 조종현; 박미희; 김선영

doi:10.9799/ksfan.2022.35.6.513

블루베리와 아로니아를 함유한 청국장 환 제품의 마우스 염증성 장질환 개선 효과
Effect of Cheonggukjang Pills Product Containing Blueberry and Aronia in Mouse Inflammatory Bowel Disease 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.35 no.6, 2022년, pp.513 - 520

김하림 ((재)전주농생명소재연구원) , 노은미 ((재)전주농생명소재연구원) , 이승현 ((재)전주농생명소재연구원) , 조종현 (순창문옥례식품(주)) , 박미희 ((재)전주농생명소재연구원) , 김선영 ((재)전주농생명소재연구원)

Abstract ▼ AI-Helper

The purpose of this study was to investigate the effect of Cheonggukjang pills with aronia and blueberries on dextran sulfate sodium (DSS)-induced colitis in mice. There have been several reports that Cheonggukjang is effective for intestinal health, but the efficacy of Cheonggukjang containing fruits has not yet been reported. In this study, we showed the effect of cheonggukjang pills with blueberries and aronia (CPBA) on DSS-induced colitis in BALB/c mice. CPBA was obtained from Soonchang Moonokae foods and orally administered once a day for 2 weeks before DSS treatment. Colitis was induced in mice by feeding 5% (w/v) DSS drinking water for 7 days. The results showed that CPBA treatment significantly alleviated DSS-induced disease activity index associated with a decrease in colon length. CPBA improved DSS-induced histological changes and intestinal epithelial barrier integrity in mice colon. In addition, CPBA administration significantly reduced the levels of DSS-mediated interferon-γ and interleukin-6 in serum and tumor necrosis factor-α in colon tissue. Moreover, the gene expression of COX-2 and iNOS, which are factors involved in inflammatory signaling, was significantly reduced by CPBA treatment. These results suggest that CPBA have a protective effect against DSS-induced mice colitis and may be a candidate for colitis treatment.

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표/그림 (6)

표 Table 1. Disease activity index
표 Table 2. Primer sequence
그림 Fig. 1. Effect of CPBA on DAI and colon length. (A) Time schedule of this study. DAI was measured daily during 5% DSS administration (B). Large intestines were obtained after the DSS administration (C) and their lengths was measured (D). Data shown are the mean±S.D. *Indicates a significant difference from the N group (***p<0.001). #Indicates a significant difference from the NC group (^##p<0.01, ^###p<0.001). N, group received water without DSS; NC, group received 5% DSS in drinking water for 7 days; PC, group pre-treated with 5-ASA for 2 weeks and then, received 5% DSS and oral administration with 5-ASA for 7 days; CPBA, group pre-treated with CPBA for 2 weeks and then, received 5% DSS and oral administration with CPBA for 7 days.
그림 Fig. 2. Effect of CPBA on the histological pathology of colitis. Histopathological change of large intestine. The rectum of mouse was stained with hematoxylin and eosin (HE) or alcian blue (AB). The upper figure shows representative HE staining images of the N, NC, PC and CPBA groups. The lower figure presents the AB staining images. N, group received water without DSS; NC, group received 5% DSS in drinking water for 7 days; PC, group pre-treated with 5-ASA for 2 weeks and then, received 5% DSS and oral administration with 5-ASA for 7 days; CPBA, group pre-treated with CPBA for 2 weeks and then, received 5% DSS and oral administration with CPBA for 7 days.
그림 Fig. 3. Effect of CPBA on the level of pro-inflammatory cytokines in plasma and in colorectal tissue. The levels of plasma (A) IL-6 and (B) IFN-γ were determined in experimental groups. (C) TNF-α mRNA expression in colorectal region was measured by qRT-PCR. Data shown are the mean±S.D. *Indicates a significant difference from the N group (*p<0.05, ***p<0.001). ^#Indicates a significant difference from the NC group (^#p<0.05, ^##p<0.01, ^###p<0.001). N, group received water without DSS; NC, group received 5% DSS in drinking water for 7 days; PC, group pre-treated with 5-ASA for 2 weeks and then, received 5% DSS and oral administration with 5-ASA for 7 days; CPBA, group pre-treated with CPBA for 2 weeks and then, received 5% DSS and oral administration with CPBA for 7 days.
그림 Fig. 4. Effect of CPBA on the expression of inflammatory genes in colorectal tissue of mice. iNOS (A) and COX-2 (B) mRNA expression in the large intestine were analyzed by qRT-PCR. Data shown are the mean±SD. *Indicates a significant difference from the N group (**p<0.01, ***p<0.001). ^#Indicates a significant difference from the NC group (^#p<0.05, ^##p<0.01, ^###p<0.001). N, group received water without DSS; NC, group received 5% DSS in drinking water for 7 days; PC, group pre-treated with 5-ASA for 2 weeks and then, received 5% DSS and oral administration with 5-ASA for 7 days; CPBA, group pre-treated with CPBA for 2 weeks and then, received 5% DSS and oral administration with CPBA for 7 days.

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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