[논문]침향 추출물의 면역조절 및 생리활성 분석

황유림; 김광연; 유선녕; 박광일; 안순철

doi:10.14374/hfs.2022.30.4.249

침향 추출물의 면역조절 및 생리활성 분석
Evaluation of Immunomodulatory and Biological Effects of Aquilaria crassna Extracts 원문보기

大韓韓醫學方劑學會誌 = Herbal formula science, v.30 no.4, 2022년, pp.249 - 257

황유림 (부산대학교 의과대학 미생물학 및 면역학 교실) , 김광연 (한국한의학연구원 한의기술응용센터) , 유선녕 (부산대학교 의과대학 미생물학 및 면역학 교실) , 박광일 (경상대학교 수의과대학 수의생리학) , 안순철 (부산대학교 의과대학 미생물학 및 면역학 교실)

Abstract ▼ AI-Helper

Objectives : Aquilaria crassna is a traditional herbal medicine, which is used to treat allergies, diabetes, neurological diseases. Recently, Aquilaria crassna extracts have been reported in anti-bacterial and anti-inflammatory activities. In this study, various solvents fraction of Aquilaria crassna were investigated on various physiological activities. Methods : According to the polarity, the solvents fraction of Aquilaria crassna were confirmed through TLC, and the activities of the extracts were confirmed in anti-diabetes, anti-obesity, whitening, anti-gout, and anti-inflammation. Results : TLC results showed that ACM and ACM/E have similar patterns and most of the components were transferred to ACM/E. Treatment with ACM and ACM/E fraction were significantly decreased the generation of NO in lipopolysaccharide (LPS)-stimulated macrophage cells. Analysis of biological activities such as α-glucosidase, protein tyrosine phosphatase (PTP1B), tyrosinase, xanthine oxidase (XO) and pancreatic lipase inhibition, showed that ACM and ACM/E have more inhibitory effects than other fractions. Conclusions : Therefore, the results of the present study clearly demonstrate that Aquilaria crassna and its constituents might be beneficial in the prevention or treatment of immune-regulating effects.

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표/그림 (4)

$Fig. 1. Solvent extraction of active compounds from Aquilaria crassna. (A) Schematic procedure for preparation. (B) Thin layer chromatography (TLC) of solvent fractions from Aquilaria crassna under UV light. (C) After colorization with anisaldehyde sulphuric acid reagent. Solvent fractions were loaded on a base line of a silica gel normal-phase TLC plate. Chloroform/methanol/water(4:2:1, v/v/v) was used as the development solvent for normal-phase TLC. ACM; methanol extract from A. crassna, ACM/E; ethyl acetate layer of ACM, ACM/b; n-butanol layer of ACM, ACM/W; water layer of ACM.$ 그림 Fig. 1. Solvent extraction of active compounds from Aquilaria crassna. (A) Schematic procedure for preparation. (B) Thin layer chromatography (TLC) of solvent fractions from Aquilaria crassna under UV light. (C) After colorization with anisaldehyde sulphuric acid reagent. Solvent fractions were loaded on a base line of a silica gel normal-phase TLC plate. Chloroform/methanol/water(4:2:1, v/v/v) was used as the development solvent for normal-phase TLC. ACM; methanol extract from A. crassna, ACM/E; ethyl acetate layer of ACM, ACM/b; n-butanol layer of ACM, ACM/W; water layer of ACM.
그림 Fig. 2. Effects of Solvent extraction of active compounds from Aquilaria crassna on nitric oxide (NO) production and inhibition in RAW 264.7 cells. (A) Production of NO treated with extracts for 24 h. (B) Cell viability treated with extracts alone for 24 h. (B) Inhibition effects of NO treated with extracts for 24 h. (D) Cell viability co-treated with extracts and LPS for 24 hr. LPS (1 μg/ml) was used as a positive. Data are represented the mean ±SD (n=3 in each group) from three separate experiments. ***p<0.001, compared with negative control. ACM, methanol extract from A. crassna; ACM/E, ethyl acetate layer of ACM; ACM/b, n-butanol layer of ACM; ACM/W, water layer of ACM.
그림 Fig. 3. Effects of Solvent extraction of active compounds from Aquilaria crassna on α-Glucosidase and PTP1B inhibition. (A) α-Glucosidase inhibitory activity, (B) PTP1B inhibitory activity, Data are represented the mean ±SD (n=3 in each group) from three separate experiments. **p<0.01, and ***p<0.001, compared with negative control. ACM, methanol extract from A. crassna; ACM/E, ethyl acetate layer of ACM; ACM/b, n-butanol layer of ACM; ACM/W, water layer of ACM. Acarbose and ursolicacid were used as a positive control for each assay, respectively.
그림 Fig. 4. Various biological activities of Solvent extraction of active compounds from Aquilaria crassna. (A) tyrosinase inhibitory activity, (B) xanthine oxidase inhibitory activity, (C) pancreatic lipase inhibitory activity. Data are represented the mean ±SD (n=3 in each group) from three separate experiments. ***p<0.001, compared with negative control. ACM, methanol extract from A. crassna; ACM/E, ethyl acetate layer of ACM; ACM/b, n-butanol layer of ACM; ACM/W, water layer of ACM. Acarbose, ursolicacid, orlistat, kojic acid, and allopurinol were used as a positive control for each assay, respectively.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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