[논문]전염성근괴사증바이러스(IMNV)를 인위감염 시킨 흰다리새우(Litopenaeus vannamei)의 근육에서 나타난 미세 손상과 염증반응에 대한 조직병리학적 특성 연구

이효은; 김영숙; 장진현; 천원주; 최가영; 김수미

doi:10.7847/jfp.2022.35.2.167

전염성근괴사증바이러스(IMNV)를 인위감염 시킨 흰다리새우(Litopenaeus vannamei)의 근육에서 나타난 미세 손상과 염증반응에 대한 조직병리학적 특성 연구
Histopathological features of pacific whiteleg shrimp, Litopenaeus vannamei, infected with Infectious Myonecrosis Virus (IMNV) with an emphasis on micro-traumas and inflammatory responses in muscle tissues 원문보기

韓國魚病學會誌= Journal of fish pathology, v.35 no.2, 2022년, pp.167 - 176

이효은 (국립수산물품질관리원 수산방역과) , 김영숙 (국립수산물품질관리원 수산방역과) , 장진현 (국립수산물품질관리원 수산방역과) , 천원주 (국립수산물품질관리원 수산방역과) , 최가영 (국립수산물품질관리원 수산방역과) , , 김수미 (국립수산물품질관리원 수산방역과)

Abstract ▼ AI-Helper

We injected infectious myonecrosis virus (IMNV) to pacific whiteleg shrimp, Litopenaeus vannamei, and observed closely with using light microscope and transmission electron microscope (TEM) for 4-8 days post infection (dpi). As clinical signs, abdominal bodies had mild opaque muscles at 5 dpi. And the mortality was shown at 6 dpi. At 8 dpi, most injected shrimps had severe opaque muscles and humped back that cause of movement disorder. As results of histopathological examinations, local parts of abdominal body muscle had muscle fiber hyalinization, muscle fiber atrophy, rounded muscle fibers, myofibrillar hypertrophy in size, a decrease in number of myofibrils and phagocytosis from the sarcolemmas by multiple hemocytes at 4 dpi. Especially, myofibrillar hypertrophy appeared at the whole or random part of single muscle fiber not in specific locations like the center or edge of muscle fiber. At 6-7 dpi, multiple muscle necrosis, muscle fiber segmentation, myofibril lysis ap- peared and a few hemocytes were infiltrated at lesions. At 8 dpi, extensive muscle necrosis, multiple myofibril lysis and muscle fiber atrophy were shown, and very few hemocytes were infiltrated. In early stage of infection, local viral myositis with zenker's degeneration were shown. These lesions appeared multiply after the early stage. In late stage of infection, extensive coagulative muscle necrosis appeared with few of inflammatory response such as hemocytes infiltration. The lack of hemocytes infiltration response at the late stage might be disadvantage for Litopenaeus vannamei to defense against IMNV and to recover, because hematocytes (granulocyte, semi-granulocyte) eliminate pathogen and damaged tissues from infection sites and help recover. As results of the TEM observation, IMNVs that had nonenveloped icosahedral capsid which was 30-40 nm diameter were in myofibril and beside tubules of sarcoplasmic reticulum and moved to the certain direction. The micro-tears and micro-trau- mas in myofibrils caused muscle fiber necrosis. And semi-granulocytes engulfed IMNVs to eliminate virus.

주제어

표/그림 (6)

표 Table 1. PCR, RT-PCR and qPCR conditions
그림 Fig. 1. Control group and IMNV infected L. vannamei. A: Control group, B: 4 dpi, C: 5 dpi, D: 6 dpi, E: 7 dpi, F and G: 8 dpi of infected shrimps. F: A cross section of abdominal body, the whole body showed opaque muscle. Arrow: opaque muscle, arrow head: humped back
그림 Fig. 2. Fine muscle structure of control group and muscular lesions at abdominal body of IMNV infected L. vannamei. A: Control group, normal muscle fibers are full of myofibrils, B and C: 4 dpi infection group, hypertrophied myofibrils are shown, some muscle fibers are hyalinized and become round because of reduced diameter, clear spaces are surrounded, some hemocytes are infiltrated between affected muscle fibers. D: 5 dpi infection group, affected myofibers are segmented and eliminated by surrounded multiple hemocytes. E: 6 dpi infection group and F: 7 dpi infection group, dotted lines identify the boundaries between coagulation necrosis and normal muscle fibers. G and H: 8 dpi infection group, extensive muscle necrosis and atrophy are shown, affected muscle fibers are lysed. Asterisk: myofibrils hypertrophy, arrow: hemocytes infiltration, arrow head: hyalinization, blank arrow: muscle fiber lysis. H&E stain.
그림 Fig. 3. IMN virus (V) at 8 dpi of infection group. A: IMNVs have icosahedral shape and are located in myofibril (M). B: IMNVs are spilled out. C and D show the details of boxed areas. TEM.
그림 Fig. 4. IMN virus (V) at 8 dpi of infection group. A: multiple various sized tubules of sarcoplasmic reticulum are in a row beside IMNV and B shows the details of the boxed area, C: Various sized tubules are beside IMNVs, D: many IMN virus are beside multiple tubules. Arrow: tubule of sarcoplasmic reticulum. TEM.
그림 Fig. 5. The engulfing of IMN virus (V) by a semigranulocyte. A: The semi-granulocyte is located beside myofibril (M) and has phagosomes full of IMNV particles. B shows the details of the boxed area. G: small granule, m: mitochondria, N: nucleus. TEM.

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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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