[논문]Antioxidant and Immune-Modulating Activities of Egg Yolk Protein Extracts

Lee, Jae Hoon; Lee, Yunjung; Paik, Hyun-Dong; Park, Eunju

doi:10.5851/kosfa.2022.e3

[국내논문] Antioxidant and Immune-Modulating Activities of Egg Yolk Protein Extracts 원문보기

Food science of animal resources, v.42 no.2, 2022년, pp.321 - 331

Lee, Jae Hoon (Research Group of Food Processing, Korea Food Research Institute) , Lee, Yunjung (Department of Food and Nutrition, Kyungnam University) , Paik, Hyun-Dong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) , Park, Eunju (Department of Food and Nutrition, Kyungnam University)

Abstract ▼ AI-Helper

Egg yolk is widely used to extract lecithin, which is utilized in the food and cosmetics industry. After lecithin is removed, the rest of egg yolk is generated as a by-product. Thus, it is necessary to properly utilize it. In this study, egg yolk protein extracts were produced using ethanol (EYE-E) and water (EYE-W). Their antioxidant and immunomodulatory effects were then evaluated. Antioxidant activities of EYE-E and EYE-W were determined using cellular antioxidant capacity (CAC) assay and comet assay. EYE-E and EYE-W showed significant (p<0.05) scavenging effects on intracellular reactive oxygen species (ROS) in a dose dependent manner. At a concentration of 50 ㎍/mL, EYE-W showed higher (p<0.05) antioxidant activity than EYE-E. EYE-E and EYE-W also exhibited protective effects against DNA damage caused by oxidative stress. After treatment with EYE-E and EYE-W, DNA damage level of 48.7% due to oxidative stress was decreased to 36.2% and 31.8% levels, respectively. In addition, EYE-E and EYE-W showed immunomodulatory effects by regulating Th1 cytokines (TNF-α and IL-2) and Th2 cytokines (IL-10 and IL-4) in Balb/c mouse splenocytes. These data suggest that EYE-E and EYE-W could be used as functional food ingredients with excellent antioxidant and immunomodulatory activities in the food industry.

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표/그림 (3)

그림 Fig. 1. Cellular antioxidant capacity (CAC) of (A) EYE-E, (B) EYE-W, and (C) 50 μg/mL of egg yolk protein extracts. Data are presented as means±SD of triplicate measurements. Various corresponding letters indicate significant differences (p<0.05) by Duncan's multiple range test. NC (negative control), treated with HBSS buffer; PC (positive control), treated with 80 mM AAPH, EYE-E, egg yolk protein extractethanol; EYE-W, egg yolk protein extract-water; AAPH, 2,2′-Azobis (2-methylpropionamidine) dihydrochloride.
그림 Fig. 2. Egg yolk protein extracts protect cells against DNA damage. Data are presented as means±SD of triplicate measurements. Various corresponding letters indicate significant differences (p<0.05) by Duncan's multiple range test. NC (negative control), treated with PBS buffer; PC (positive control), treated with 200 μM H2O2; EYE-E, 50 μg/mL of egg yolk protein extract-ethanol; EYE-W, 50 μg/mL of egg yolk protein extract-water.
그림 Fig. 3. Effects of EYE-E and EYE-W on Th1 and Th2 type cytokines from splenocytes in Balb/c mouse treated with LPS or ConA. Data are presented as means±SD of triplicate measurements. Various corresponding letters indicate significant (p<0.05) differences by Duncan's multiple range test. NC (negative control), cell only; LPS or ConA, treated with LPS (5 μg/mL) or ConA (5 μg/mL); EYE-E, 200 μg/mL of egg yolk protein extract-ethanol; EYE-W, 200 μg/mL of egg yolk protein extract-water; ConA, concanavalin A.

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대상 데이터

The water extract was directly freeze dried for 3 days. Samples were classified as EYE-E (egg yolk protein extract-ethanol) and EYE-W (egg yolk protein extract-water).

데이터처리

The results were compared by analysis of one-way ANOVA followed by Duncan's multiple-range test using SPSS for Window ver

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