[논문]총백추출물의 고지방식이 유도 비만 마우스에서의 항비만 효과 및 근육조직에서의 에너지대사 조절기전 연구

최윤용; 이현수; 백수연; 임수민; 정효원; 강석용; 박용기

doi:10.15429/jkomor.2022.22.2.102

총백추출물의 고지방식이 유도 비만 마우스에서의 항비만 효과 및 근육조직에서의 에너지대사 조절기전 연구
Anti-Obesity Effects and the Regulation of Energy Metabolism in Skeletal Muscle Tissues of Allii Fistulosi Bulbus Extract in High Fat Diet-Induced Obesity Mice 원문보기

한방비만학회지 = Journal of Korean Medicine for Obesity Research, v.22 no.2, 2022년, pp.102 - 114

최윤용 (동국대학교 한의과대학) , 이현수 (동국대학교 한의과대학) , 백수연 (동국대학교 한의과대학) , 임수민 (동국대학교 한의과대학) , 정효원 (동국대학교 한의과대학) , 강석용 (동국대학교 한방신약개발센터) , 박용기 (동국대학교 한의과대학)

Abstract ▼ AI-Helper

Objectives: We investigated the effects of Allii Fistulosi Bulbus (AFB) on high fat diet (HFD)-induced obesity in mice and the regulation of energy metabolism in muscle tissues of mice. Methods: The C57BL/6 mice (6 weeks, male) were fed a HFD for 8 weeks and then administrated with AFB extract at 500 mg/kg (p.o.) once daily for 4 weeks. The body weight (BW), muscle weight, calorie intake, fasting blood glucose (FBG) and serum glucose, insulin, and low-density lipoprotein-cholesterol (LDL-C) levels were measured in mice. It was also observed the histological changes of pancreas, liver, and fat tissues with hematoxylin and eosin staining. It was investigated the phosphorylation of insulin receptor substrate 1 (IRS-1), Ser/Thr kinase (AKT), and adenosine monophosphate-activated protein kinase (AMPK), and the expression of phosphoinositide 3-kinase, glucose transporter type 4 (GLUT4), and sirtuin1 (Sirt1) in gastrocnemius tissues by western blot, respectively. Results: The increases of BWs, calorie intakes and FBG levels in obesity mice were decreased significantly by the administration of AFB extract. The AFB extract administration was reduced significantly serum levels of glucose, insulin, and LDL-C in obesity mice. The AFB extract inhibited lipid accumulation in liver tissues, hyperplasia of pancreatic islets, and enlargement of fat tissues in obesity mice. The phosphorylation of IRS-1 and AKT was increased significantly in muscle tissues and AMPK phosphorylation and the GLUT4 and Sirt1 expression were decreased significantly in muscle tissues after the AFB administration. Conclusions: Our study indicates that AFB extract improves symptoms of obesity through regulation of energy regulating proteins in muscle tissues.

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표/그림 (5)

그림 Fig. 1. Effects of AFB extract on the changes of body weight, food intake, and calorie intake in HFD-induced obesity mice. (A) Body weights were measured in mice once a week during drug administration. (B) Body weights gain was calculated using body weights at 9-12 week. (C) Calorie intake was calculated using food intakes at 9-12 week. Data were presented as mean±standard deviation (n=5 per a group). *P<0.05, **P<0.01, and ***P<0.001 vs. Nor (a) or HFD group (b). Nor: normal group, HFD: high fat diet-induced obesity control group, AFB: Allii Fistulosi Bulbus extract (500 mg/kg)-administrated group, Met: metformin (500 mg/kg)-administrated group.
그림 Fig. 2. Effects of AFB extract on the blood glucose levels in HFD-induced obesity mice. The fasting blood glucose levels were measured in whole blood of mice at 8-12 weeks (A) and were presented as a histogram at 12 weeks (B). The oral glucose tolerance test were measured at 12 week (C), and the area under curve was calculated using a GraphPad Prism 5.0 program (GraphPad Software, La Jolla, CA, USA) (D). Data were presented as mean±standard deviation (n=5 per a group). *P<0.05, **P<0.01, and ***P<0.001 vs. Nor (a) or HFD group (b). Nor: normal group, HFD: high fat diet-induced obesity control group, AFB: Allii Fistulosi Bulbus extract (500 mg/kg)-administrated group, Met: metformin (500 mg/kg)-administrated group.
그림 Fig. 3. Effects of AFB extract on the levels of glucose, insulin, and LDL-cholesterol in the sera of HFD-induced obesity mice. The levels of (A) glucose, (B) insulin, and (C) LDL-cholesterol were measured in the sera of mice at 12 weeks. Data were presented as mean±standard deviation (n =5 per a group). **P<0.01, and ***P<0.001 vs. Nor (a) or HFD group (b). Nor: normal group, HFD: high fat diet-induced obesity control group, AFB: Allii Fistulosi Bulbus extract (500 mg/kg)-administrated group, Met: metformin (500 mg/kg)-administrated group, LDL: low-density lipoprotein.
그림 Fig. 4. Effects of AFB extract on the histological changes of liver and adipose tissues in HFD-induced obesity mice. The liver (A), pancreas (C), epididymis fat (D), and brown fat (E) tissues were stained with hematoxylin and eosin and then were observed under microscope (x100). (B) The weights of liver were measured in mice at 12 week. Data were presented as mean±standard deviation (n=5 per a group). **P<0.01, and ***P<0.001 vs. Nor (a) or HFD group (b). Nor: normal group, HFD: high fat diet-induced obesity control group, AFB: Allii Fistulosi Bulbus extract (500 mg/kg)-administrated group, Met: metformin (500 mg/kg)-administrated group. Arrows were shown pathological changes of each tissue.
그림 Fig. 5. Effects of AFB extract on muscle weights and the expression of energy metabolism-regulating proteins in gastrocneminus tissues of HFD-induced obesity mice. (A) The weights of gasctrocnemius tissues of mice were mearued at 12 weeks. The expression of p-IRS-1, IRS-1 (B), p-AKT, AKT (C), PI3K (D), p-AMPK, AMPK (E), GLUT4 (F), Sirt1 (G), and β-actin was determined by Western blot, respectively. β-actin was used an internal control. Histograms (A-G) were analyzed the expression of target protein per each total form or β-actin. Data were presented as mean±standard deviation (n=5 per a group). *P<0.05, **P<0.01, and ***P<0.001 vs. Nor: normal group, HFD: high fat diet-induced obesity control group, AFB: Allii Fistulosi Bulbus extract (500 mg/kg)-administrated group, Met: metformin (500 mg/kg)-administrated group, IRS-1: insulin receptor substrate 1, AKT: Ser/Thr kinase, PI3K: phosphoinositide 3-kinase, AMPK: adenosine monophosphate-activated protein kinase, GLUT4: Glucose transporter type 4, Sirt1: sirtuin1.

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