[논문]심황색소의 감광활성과 빛 조사에 의한 지질산화 및 세포독성 유도 효과

김희정; 홍정일

doi:10.9721/kjfst.2022.54.1.59

심황색소의 감광활성과 빛 조사에 의한 지질산화 및 세포독성 유도 효과
Induction of lipid peroxidation and melanoma cell death by turmeric oleoresin through its photosensitizing properties 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.54 no.1, 2022년, pp.59 - 65

김희정 (서울여자대학교 자연과학대학 식품응용시스템학부) , 홍정일 (서울여자대학교 자연과학대학 식품응용시스템학부)

초록
AI-Helper

본 연구에서는 형광등 빛 조사 하에서 심황색소의 감광활성을 평가하고 이로 인한 유지산화 유도 및 세포독성 강화 효과를 평가하였다. Formazan 탈색 및 NBT 환원을 이용한 실험에서 심황색소는 빛에 노출 시 유의적인 감광활성을 보였다. 또한, 심황색소는 빛 조사에 의해 올리브유를 제외한 옥수수유, 카놀라유와 대두유 등 식용유지에서 hydroperoxide 양과 TBARS 양을 현저하게 증가시켰다. 그러나, 심황색소는 빛 조사 하에서 O/W 반응계에서 linoleic acid로부터 TBARS의 생성을 저해했으며, ROS에 의한 DCF 형광반응도 감소시키는 산화방지 효과를 나타냈다. 또한 심황색소의 흑색종에 대한 세포독성도 형광등 빛 조사에 의해 현저하게 강화되었다. 본 연구결과는 식품 첨가물로 사용되는 심황색소가 감광활성 발현을 통해 특정환경에서 유지산화를 유도하는 부정적 영향을 미칠 수 있으나, 암세포 사멸 효과강화 등의 긍정적인 활성을 나타낼 수 있음을 보여준다.

Abstract ▼ AI-Helper

Curcuminoids in turmeric oleoresin (TO) are known to be effective antioxidants; they exhibit photosensitizing properties under light. In this study, the photoreactive properties of TO and its consequent induction of lipid peroxidation and cytotoxicity were evaluated. TO exhibited photosensitizing activities as evidenced by the reduction of nitro blue tetrazolium and by the decolorization of formazan under light, whereas light-irradiated TO did not enhance the levels of reactive oxygen species. The levels of hydroperoxide and thiobarbituric acid reactive substance (TBARS) were significantly elevated under a fluorescent light (10 W/m2) in corn, canola, and soybean oils containing 10-40 and 20-80 ㎍/mL of TO (p<0.05) but not in olive oil. Canola oil was the most sensitive to photo-oxidation induced by TO. The level of TBARS from linoleic acid in the oil in water system was, however, decreased by TO under light. The cytotoxicity effect of TO on melanoma cells was also substantially enhanced under light.

주제어

표/그림 (6)

그림 Fig. 1. Evaluation of photo-reactivity of TO based on the abilities of NBT reduction and formazan decolorization. Different concentrations (μg/mL) of TO were incubated with NBT in the absence or presence of methionine under 10 W/m² light or in a dark during 24 h at 25°C (A). TO and EB were incubated with MTT formazan under 10 W/m² light for 24 h (B). Half-life (a period that requires 50% formazan degradation) changes of MTT (C) and TTC (D) formazan by TO under 2.5, 5, and 10 W/m² lights were calculated. Each value represents the mean±SD (n=3). Different lowercase (A-D) and uppercase (C and D) letters indicate significant difference among different concentrations and different light intensity, respectively (p<0.05) based on one way ANOVA and the Tukey's HSD test. N/C; not calculated.
그림 Fig. 2. Hydroperoxide level formed in commercial oils containing TO by light irradiation. Hydroperoxide level was analyzed in corn oil (A), canola oil (B), soybean oil (C), and olive oil (D) containing 10-40 μg/mL TO after irradiation of 10 W/m² light or in a dark for 1 h. Each value represents the mean±SD (n=3). Different letters indicate significant difference (p<0.05) based on one way ANOVA and the Tukey's HSD test. n.s; not significant. ^## significantly different between dark and light control according to Student's t-test (^##p<0.01)
그림 Fig. 3. Effects of TO on TBARS formation in commercial oils under light. TBARS level in corn oil (A), canola oil (B), soybean oil (C), and olive oil (D) containing TO was analyzed after 4 h irradiation of 10 W/m² light or in a dark. Each value represents the mean±SD (n=3). Different letters indicate significant difference (p<0.05) based on one way ANOVA and the Tukey's HSD test. n.s; not significant.
그림 Fig. 4. Effects of TO on lipid peroxidation in linoleic acid/SDS (O/W) system under light. Levels of hydroperoxide (A) and TBARS (B) in linoleic acid/SDS (0.2/0.4%) emulsion system containing TO were analyzed after 1 h (A) or 2 h (B) irradiation of 10 W/m² light or in a dark. Each value represents the mean±SD (n=3). Different letters indicate significant difference (p<0.05) based on one way ANOVA and the Tukey's HSD test. ^{#, ##} significantly different between dark and light control according to Student's t-test (^#p<0.05, ^##p<0.01)
그림 Fig. 5. Effects of TO on DCF fluorescence under light. DCFH mixed with different concentrations of TO or EB was incubated under 10 W/m² light during 240 min (A). Changes in DCF fluorescence in a dark were also analyzed (B). Each value represents the mean±SD (n=3). Different letters indicate significant difference (p<0.05) based on one way ANOVA and the Tukey's HSD test. n.s; not significant.
그림 Fig. 6. Changes in cytotoxic effects of TO on melanoma cells by light irradiation. B16F10 cells were treated with different concentrations of TO and incubated under 5 W/m² light or a dark at 37°C, 5% CO₂ for 1 h. The cells were then further incubated for 24 h with or without changing media, and the cell viability was analyzed using the MTT assay (A). IC₅₀ values were also calculated (B). Each value represents the mean±SD (n=6). Different letters indicate significant difference (p<0.05) based on one way ANOVA and the Tukey's HSD test.

참고문헌 (24)

Abrahamse H, Hamblin MR. New photosensitizers for photodynamic therapy. Biochem. J. 473: 347-364 (2016)

상세보기
Amalraj A, Pius A, Gopi S, Gopi S. Biological activities of curcuminoids, other biomolecules from turmeric and their derivatives-A review. J. Tradit. Complement. Med. 7: 205-233 (2017)

상세보기
Awolu O, Oladeji O. Natural plant pigments and derivatives in functional foods developments. Eurasian J. Food Sci. Technol. 5: 25-40 (2021)
Bacellar IOL, Baptista MS. Mechanisms of photosensitized lipid oxidation and membrane permeabilization. ACS Omega 4: 21636-21646 (2019)

상세보기
Buss S, Dobra J, Goerg K, Hoffmann S, Kippenberger S, Kaufmann R, Hofmann M, Bernd A. Visible light is a better co-inducer of apoptosis for curcumin-treated human melanoma cells than UVA. Plos One 8: e79748 (2013)

상세보기
Hong J, Kang S, Jung YN. Formulation for measuring photosensitizing activity, and kit and method using same. Korea Patent 10-1850483 (2018)
Jankun J, Wyganowska-Swiatkowska M, Dettlaff K, Jelinska A, Surdacka A, Watrobska-Swietlikowska D, Skrzypczak-Jankun E. Determining whether curcumin degradation/condensation is actually bioactivation. Int. J. Mol. Med. 37: 1151-1158 (2016)

상세보기
Jung YN, Hong J. Changes in chemical properties and bioactivities of turmeric pigments by photo-degradation. AIMS Agriculture and Food 6: 754-767 (2021)

상세보기
Kang S. Development of a rapid and convenient method for evaluating photosensitizing activity using formazan dyes and effects of various solvent agents on bioactivities of curcuminoids. PhD thesis, Seoul Women's University, Seoul, Korea (2021)
Kwiatkowski S, Knap B, Przystupski D, Saczko J, Kedzierska E, Knap-Czop K, Kotlinska J, Michel O, Kotowski K, Kulbacka J. Photodynamic therapy-mechanisms, photosensitizers and combinations. Biomed. Pharmacother. 106: 1098-1107 (2018)

상세보기
Lee E, Hong J. Reduction of nitro blue tetrazolium by combined reaction of various photosensitizers with amino acids. Korean J. Food Sci. Technol. 54: 1-7 (2022)
Mahmood K, Zia KM, Zuber M, Salman M, Anjum MN. Recent developments in curcumin and curcumin based polymeric materials for biomedical applications: A review. Int. J. Biol. Macromol. 81: 877-890 (2015)

상세보기
Min DB, Boff JM. Chemistry and reaction of singlet oxygen in foods. Compr. Rev. Food Sci. F. 1: 58-72 (2002)

상세보기
Moon JS, Lee OH, Son JY. The oxidation stability of virgin and pure olive oil on autoxidation and thermal oxidation. J. Korean Soc. Food Sci. Nutr. 34: 93-98 (2005)

원문보기 상세보기
Peram MR, Jalalpure SS, Palkar MB, Diwan PV. Stability studies of pure and mixture form of curcuminoids by reverse phase-HPLC method under various experimental stress conditions. Food Sci. Biotechnol. 26: 591-602 (2017)

상세보기
Rao MNA. Curcuminoids as potent inhibitors of lipid peroxidation. J. Pharm. Pharmacol. 46: 1013-1016 (1994)

상세보기
Ryu KY, Kim AG, Kim TS, Lee HH, Seo KW, Cho BS. Inhibition effect of herbs on the rancidity of soybean oil. Korean J. Food Preserv. 25: 36-43 (2018)

원문보기 상세보기
Song ES, Kang S, Hong J. Changes in chemical properties, antioxidant activities, and cytotoxicity of turmeric pigments by thermal process. Korean J. Food Sci. Technol. 50: 21-27 (2018)

원문보기 상세보기
Szlasa W, Supplitt S, Drag-Zalesinska M, Przystupski D, Kotowski K, Szewczyk A, Kasperkiewicz P, Saczko J, Kulbacka J. Effects of curcumin based PDT on the viability and the organization of actin in melanotic (A375) and amelanotic melanoma (C32)-in vitro studies. Biomed. Pharmacother. 132: 110883 (2020)

상세보기
Wang Z, Jia Y, Li W, Zhang M. Antimicrobial photodynamic inactivation with curcumin against Staphylococcus saprophyticus, in vitro and on fresh dough sheet. LWT-Food Sci. Technol. 147: 111567 (2021)

상세보기
Yang MY, Chang CJ, Chen LY. Blue light induced reactive oxygen species from flavin mononucleotide and flavin adenine dinucleotide on lethality of HeLa cells. J. Photoch. Photobio. B. 173: 325-332 (2017)

상세보기
Yi B, Ka HJ, Kim MJ, Lee J. Effects of curcumin on the oxidative stability of oils depending on type of matrix, photosensitizers, and temperature. J. Am. Oil Chem. Soc. 92: 685-691 (2015)

상세보기
Yi B, Kim MJ, Lee J. Lipid oxidation and antioxidant mechanisms in different matrix. Food Sci. Ind. 51: 127-135 (2018)

원문보기 상세보기
Zhang YP, Li YQ, Lv YT, Wang JM. Effect of curcumin on the proliferation, apoptosis, migration, and invasion of human melanoma A375 cells. Genet. Mol. Res. 14: 1056-1067 (2015)

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증