[논문]Bacillus amyloliquefaciens ISP-5 균주의 배지 최적화 및 상추를 이용한 식물 생장 촉진 평가

최강현; 서선일; 박해성; 임지환; 김평일

doi:10.5010/jpb.2022.49.4.356

Bacillus amyloliquefaciens ISP-5 균주의 배지 최적화 및 상추를 이용한 식물 생장 촉진 평가
Medium optimization for growth of Bacillus amyloliquefaciens ISP-5 strain and evaluation of plant growth promotion using lettuce 원문보기

Journal of plant biotechnology = 식물생명공학회지, v.49 no.4, 2022년, pp.356 - 361

최강현 ((재)농축산용미생물산업지원육성센터) , 서선일 ((재)농축산용미생물산업지원육성센터) , 박해성 ((재)농축산용미생물산업지원육성센터) , 임지환 ((재)농축산용미생물산업지원육성센터) , 김평일 ((재)농축산용미생물산업지원육성센터)

초록
AI-Helper

본 연구에서는 Bacillus amyloliquefaciens ISP-5 균주의 최적배지조성을 확인하기 위해 5종의 탄소원(glucose, fructose, sucrose, maltose, corn starch)과 5종의 질소원(yeast extract, peptone, tryptone, soytone, soy bean flour)을 이용하여 각각의 생육을 측정하였다. 그 결과 glucose와 soy bean flour를 각각 최적 탄소원과 질소원으로 확립하였으며 최적 배지를 이용하였을 때 상업용 배지(TSB)에 비해 약 2500배의 생육을 확인할 수 있었다. B. amyloliquefaciens ISP-5 배양액을 상추에 엽면 살포하였을 때 상추의 엽폭과 엽장은 대조군에 비해 8.6%, 12.9%의 증가하였으며, 생체중과 건물중은 대조군에 비해 각각 24.2%, 23.9% 증가하였다. 이러한 결과들을 종합해보았을 때 B. amyloloiquefaciens ISP-5 균주를 이용한 미생물 제제는 상추용 식물생장촉진제로써 활용할 수 있을 것이라 기대된다.

Abstract ▼ AI-Helper

Bacillus sp. is a useful strain for agriculture because it promotes plant growth and controls plant pathogens through a variety of mechanisms. In this study, we obtained a microbial preparation with a high number of viable cells by culturing newly isolated soil bacteria on an optimized medium. Subsequently, we applied this preparation to lettuce to enhance its growth and yield. First, B. amyloliquefaciens ISP-5 was isolated from soil. Next, optimization of culture medium was carried out using 5 L scale fermenters. When culturing B. amyloliquefaciens ISP-5 on this optimized medium, the number of viable cells was approximately 1000 times higher than that obtained from culturing on the commercial medium. Afterwards, the plant growth promotion properties of the ISP-5 strain were evaluated using lettuce as a test plant. Foliar spray treatment of lettuce was carried out by inoculating half the standard concentration suspension (0.5 × 107 cfu/ml). As a result, leaf width increased by 8.6% and leaf length increased by 12.9% compared to the control group. Live weight also increased by 24.2% and dry weight by 23.9%. Considering the results from field test, B. amyloliquefaciens ISP-5 showed potential as a plant growth-promoting bacteria.

주제어

표/그림 (6)

그림 Fig. 1 Culture profile of Bacillus amyloliquefaciens ISP-5 on optimal medium (MMS) and TSB medium in 5 L jar fermenter
표 Table 1 Culture profile of Bacillus amyloliquefaciens ISP-5 using 5 carbon and 5 nitrogen sources
그림 Fig. 2 IAA production of Bacillus amyloliquefaciens ISP-5 in culture medium. (a) Standard curve of IAA, (b) IAA production of Bacillus amyloliquefaciens ISP-5 in (MMS?) culture medium
그림 Fig. 3 Growth promotion in lettuce by Bacillus amyloliquefaciens ISP-5 culture
표 Table 2 Growth evaluation of leaf width and leaf height in lettuce using Bacillus amyloliquefaciens ISP-5 culture
표 Table 3 Growth evaluation of live and dry weight in lettuce using Bacillus amyloliquefaciens ISP-5 culture

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