[논문]기수산 물벼룩의 유충 호르몬(Juvenile hormone) 신호전달경로 관련 유전자의 특성 분석

조하영; 유제원; 이영미

doi:10.11626/kjeb.2022.40.3.255

기수산 물벼룩의 유충 호르몬(Juvenile hormone) 신호전달경로 관련 유전자의 특성 분석
Molecular characterization of juvenile hormone signaling pathway-related genes in the brackish water flea Diaphanosoma celebensis 원문보기

환경생물 = Korean journal of environmental biology, v.40 no.3, 2022년, pp.255 - 266

조하영 (상명대학교 융합공과대학 생명공학과) , 유제원 (상명대학교 융합공과대학 생명공학과) , 이영미 (상명대학교 융합공과대학 생명공학과)

초록
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갑각류에서 탈피는 ecdysteroid와 juvenile hormone (JH) 신호 경로에 관여하는 유전자의 상호작용에 의해 조절된다. Ecdysteroid와 달리, 탈피 과정에서 JH 신호 경로 유전자의 역할은 부유성 갑각류에서는 잘 알려져 있지 않다. 본 연구는 기수산 물벼룩(Diaphanosoma celebensis)의 JH 신호경로에서 JH 합성, 수용체, 분해 등에 관여하는 3종의 유전자(JHAMT, Met, JHEH)의 염기서열 분석과 계통 분석을 실시하였다. 또한 탈피 주기에서 이들 유전자의 mRNA 발현양상을 분석하였다. D. celebensis의 JH 관련 유전자는 잘 보존된 domain을 가지고 있었으며, 아미노산 서열 분석과 계통 분석 결과는 이들 단백질이 곤충 및 다른 갑각류의 해당 단백질과 기능적으로 유사한 특징을 나타낸다. 또한 탈피 주기에 따른 유충 호르몬( JH) 신호전달경로 관련 유전자의 발현변화 결과를 통해 이들 유전자가 JH의 합성 및 분해에 관여함으로써 D. celebensis에서 성공적인 탈피에 기여할 것임을 제시하였다. 본 연구는 지각류에서 탈피 주기에서 JH 경로 유전자의 역할을 이해하는 데 도움이 될 것이다.

Abstract ▼ AI-Helper

In crustaceans, molting is regulated by interactions between ecdysteroid and juvenile hormone (JH) signaling pathway-related genes. Unlike the ecdysteroid signaling pathway, little information on the role of JH signaling pathway-related genes in molting is available in zooplanktonic crustaceans. In this study, three genes (juvenile hormone acid O-methyltransferase (JHAMT), methoprene-tolerant (Met), and juvenile hormone epoxide hydrolase (JHEH)) which are involved in the synthesis, receptor-binding, and degradation of JH were identified using sequence and phylogenetic analysis in the brackish water flea, Diaphanosoma celebensis. Transcriptional changes in these genes during the molting cycle in D. celebensis were analyzed. Sequence and phylogenetic analysis revealed that these putative proteins may be functionally conserved along with those of insects and other crustaceans. In addition, the expression of the three genes was correlated with the molting cycle of D. celebensis, indicating that these genes may be involved in the synthesis and degradation of JH, resulting in normal molting. This study will provide information for a better understanding of the role of JH signaling pathway-related genes during the molting process in Cladocera.

주제어

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