[논문]발효 시금치 추출물의 무기인산염에 의해 유도된 혈관 석회화 저해 효과

이상희; 홍선미; 성미정

doi:10.7318/kjfc/2022.37.3.248

발효 시금치 추출물의 무기인산염에 의해 유도된 혈관 석회화 저해 효과
Inhibitory Effect of Fermented Spanish Extract on Inorganic phosphate-induced Vascular Calcification in ex vivo Aortic Rings 원문보기

韓國食生活文化學會誌 = Journal of the Korean Society of Food Culture, v.37 no.3, 2022년, pp.248 - 255

이상희 (한국식품연구원) , 홍선미 (환동해산업연구원) , 성미정 (한국식품연구원)

Abstract ▼ AI-Helper

Spinach (Spinacia oleracea L.), a green leafy vegetable, is well known as a functional food due to its biological activities. Vascular calcification is associated with several disease conditions including atherosclerosis, diabetes, and chronic kidney disease (CKD), and is known to raise the risk of cardiovascular diseases related morbidity and mortality. However, there are no previous studies that have investigated the effects of fermented spinach exract (FSE) against aortic and its underlying mechanisms. Therefore, this study investigated the effects and action of possible mechanisms of FSE on inorganic phosphate (PI)-induced vascular calcification in ex vivo mouse aortic rings. PI increased vascular calcification through calcium deposition in ex vivo aortic rings. FSE inhibited calcium accumulation and osteogenic key marker, runt-related transcription factor 2 (Runx2), and bone Morphogenetic Protein 2 (BMP-2) protein expression in ex vivo aortic rings. And, FSE inhibited PI-induced extracellular signal-regulated kinase (ERK) and p38 phosphorylation in ex vivo aortic rings. These results show that FSE can prevent vascular calcification which may be a crucial way for the prevention and treatment of vascular disease association with vascular calcification.

주제어

표/그림 (5)

그림 PI increased vascular calcification in ex vivo aortic rings. Pieces of mouse aorta were cultured in calcification medium (1.4 and 2.6 mM PI) for 7 days. A. Aortic calcification, determined in consecutive sections of the aortic rings by Alizarin red staining (middle) and Von kossa (left). H&E staining (left panels) was performed for histology. Highermagnification images of the boxed areas in the middle panels are shown in the right panels. Representative images from 4 independent experiments performed in duplicate are shown. Scale bars: 100 μm (left and middle panels) and 50 μm (right panels). B Total calcium content of aortic rings in each group determined by Image J in separated sets of experiments. Bar values are means±SD (n=4, ****p<0.0001). Cont: control; PI: inorganic phosphate
표 Vitamin K1 and K2 contents using LC-MS methods
그림 Fermented Spanish Extract inhibited PI-induced vascular calcification in ex vivo aortic rings. Pieces of mouse aorta treated with none (Cont), PI 2.6 mM, PI 2.6 mM+Rapamycin 100ng/mL (PI 2.6 mM+Rapa), PI 2.6 mM+FSE 100 μg/mL (PI 2.6 mM+FSE) for 7 days. A. Aortic calcification, determined in consecutive sections of the aortic rings by Alizarin red staining (middle) and Von kossa (left). H&E staining (left panels) was performed for histology. Representative images from 4 independent experiments performed in duplicate are shown. Scale bars: 100 μm. B. Total calcium content of aortic rings in each group determined by Image J in separated sets of experiments. Bar values are means±SD (n=4, ****p<0.0001). Cont: control; PI: inorganic phosphate; PI+Ra; inorganic phosphate+rapamycin; PI+FSE: inorganic phosphate+fermented Spanish extract.
그림 Fermented Spanish Extract inhibited PI-induced Runx2, and BMP-2 in ex vivo aortic rings. Pieces of mouse aorta treated with none (Cont), PI 2.6 mM, PI 2.6 mM+Rapamycin 100 ng/mL (PI 2.6 mM+Rapa), PI 2.6 mM+FSE 100 μg/ mL (PI 2.6 mM+FSE) for 7 days. A. Runx2 and BMP2 protein expression by western blotting. B. Relative expression normalized to β-actin. Results are presented as mean±SD from three independent experiments. (n=4, ***p<0.001 compared with Cont, ^###p<0.001, ^####p<0.0001 compared with PI). Cont: control; PI: inorganic phosphate; PI+Ra; inorganic phosphate+rapamycin; PI+FSE: inorganic phosphate+fermented Spanish extract.
그림 Fermented Spanish Extract inhibited PI-induced MAPK pathway in ex vivo aortic rings. Pieces of mouse aorta treated with none (Cont), PI 2.6 mM, PI 2.6 mM+Rapamycin 100 ng/mL (PI 2.6 mM+Rapa), PI 2.6 mM+FSE 100 μg/ mL (PI 2.6 mM+FSE) for 7 days. A. phosphor-ERK and phosphor-p38 protein expression by western blotting. B. Relative expression normalized to ERK and p38. Results are presented as mean±SD from three independent experiments. (n=4, ***p<0.001, ***p<0.0001 compared with Cont, ^###p<0.001, ^####p<0.0001 compared with PI). Cont: control; PI: inorganic phosphate; PI+Ra; inorganic phosphate+rapamycin; PI+FSE: inorganic phosphate+fermented Spanish extract.

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