[논문]금은화(金銀花)물추출물이 마우스 대식세포의 사이토카인 생성에 미치는 영향

박완수

doi:10.15188/kjopp.2022.04.36.2.66

금은화(金銀花)물추출물이 마우스 대식세포의 사이토카인 생성에 미치는 영향
Effects of Flos Lonicerae Japonicae Water Extract on Cytokine Production in RAW 264.7 Mouse Macrophages 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.36 no.2, 2022년, pp.66 - 72

Abstract ▼ AI-Helper

Flos Lonicerae Japonicae (the flower buds of Lonicera japonica Thunberg) has been used as an antibacterial and antiviral drug in Korean Medicine. The aim of this study is to evaluate the effect of Flos Lonicerae Japonicae water extract (FL) on the production of cytokines in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS). After 24 h treatment, the production of various cytokines from RAW 264.7 was measured with multiplex cytokine assay using Bio-Plex 200 suspension array system. FL at concentrations of 50, 100, and 200 ㎍/mL significantly inhibited productions of tumor necrosis factor-α, macrophage inflammatory protein (MIP)-1β, and MIP-2 in LPS-stimulated RAW 264.7 cells; FL at concentrations of 100 and 200 ㎍/mL significantly inhibited productions of leukemia inhibitory factor, LIX (CXCL5), and RANTES in LPS-stimulated RAW 264.7 cells; FL at concentrations of 200 ㎍/mL significantly inhibited productions of granulocyte-macrophage colony-stimulating factor and macrophage colony-stimulating factor in LPS-stimulated RAW 264.7 cells; FL at concentrations of 50 and 100 ㎍/mL significantly increased productions of interleukin (IL)-10 in LPS-stimulated RAW 264.7 cells; FL at concentrations of 50, 100, and 200 ㎍/mL significantly increased productions of IL-6 and interferon gamma-induced protein-10 in LPS-stimulated RAW 264.7 cells; FL at concentrations of 100 and 200 ㎍/mL significantly increased productions of monocyte chemoattractant protein-1 in LPS-stimulated RAW 264.7 cells. Taken together, these data mean that FL might modulate productions of cytokines, chemokines, and growth factor in LPS-stimulated macrophages. Further study needs to verify the exact mechanism for modulatory activities of FL with macrophages.

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표/그림 (12)

그림 Fig. 1. Effects of FL on cell viability of RAW 264.7 cells for 24 h incubation. Data represent Mean ± SD. FL, water extract of the dried flower buds of Lonicera japonica. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. LPS indicates the group treated with 1 μg/mL of lipopolysaccharide. ^# p < 0.05 vs. Nor.
그림 Fig. 2. Effects of FL on TNF-α production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. FL, water extract of the dried flower buds of Lonicera japonica. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. IN means indomethacin (0.5 μM). LPS indicates the group treated with 1 μg/mL of lipopolysaccharide. ^# p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Fig. 3. Effects of FL on LIF production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. FL, water extract of the dried flower buds of Lonicera japonica. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. IN means indomethacin (0.5 μM). LPS indicates the group treated with 1 μg/mL of lipopolysaccharide. ^# p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Fig. 4. Effects of FL on LIX production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. FL, water extract of the dried flower buds of Lonicera japonica. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. IN means indomethacin (0.5 μM). LPS indicates the group treated with 1 μg/mL of lipopolysaccharide. ^# p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Fig. 5. Effects of FL on MIP-1β production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. FL, water extract of the dried flower buds of Lonicera japonica. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. IN means indomethacin (0.5 μM). LPS indicates the group treated with 1 μg/mL of lipopolysaccharide. ^# p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Fig. 6. Effects of FL on MIP-2 production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. FL, water extract of the dried flower buds of Lonicera japonica. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. IN means indomethacin (0.5 μM). LPS indicates the group treated with 1 μg/mL of lipopolysaccharide. ^# p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Fig. 7. Effects of FL on RANTES production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. FL, water extract of the dried flower buds of Lonicera japonica. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. IN means indomethacin (0.5 μM). LPS indicates the group treated with 1 μg/mL of lipopolysaccharide. ^# p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Fig. 8. Effects of FL on IL-6 production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. FL, water extract of the dried flower buds of Lonicera japonica. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. IN means indomethacin (0.5 μM). LPS indicates the group treated with 1 μg/mL of lipopolysaccharide. ^# p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Fig. 9. Effects of FL on MCP-1 production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. FL, water extract of the dried flower buds of Lonicera japonica. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. IN means indomethacin (0.5 μM). LPS indicates the group treated with 1 μg/mL of lipopolysaccharide. ^# p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Fig. 10. Effects of FL on IL-10 production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. FL, water extract of the dried flower buds of Lonicera japonica. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. IN means indomethacin (0.5 μM). LPS indicates the group treated with 1 μg/mL of lipopolysaccharide. ^# p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Fig. 11. Effects of FL on IP-10 production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. FL, water extract of the dried flower buds of Lonicera japonica. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. IN means indomethacin (0.5 μM). LPS indicates the group treated with 1 μg/mL of lipopolysaccharide. ^# p < 0.05 vs. Nor; * p < 0.05 vs. Con.
그림 Fig. 12. Effects of FL on GM-CSF production in LPS-stimulated RAW 264.7 cells for 24 h treatment. Data represent Mean ± SD. FL, water extract of the dried flower buds of Lonicera japonica. Nor, the group incubated with media only. Con, the group treated with lipopolysaccharide (1 μg/mL) only. IN means indomethacin (0.5 μM). LPS indicates the group treated with 1 μg/mL of lipopolysaccharide. ^# p < 0.05 vs. Nor; * p < 0.05 vs. Con.

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