[논문]Streptozotocin으로 유발된 흰쥐의 당뇨 증상 개선에 미치는 한약재 첨가 청국장 추출물의 영향

조창숙; 김소영; 최문열; 김미형; 고경아; 김미려; 서부일

doi:10.6116/kjh.2022.37.4.49.

Streptozotocin으로 유발된 흰쥐의 당뇨 증상 개선에 미치는 한약재 첨가 청국장 추출물의 영향
Effects of Herbal Medicine-added Cheonggugjang extract on Improvement of the Symptoms in Streptozotocin-induced Diabetic Rats 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.4, 2022년, pp.49 - 57

조창숙 (대구한의대학교 한의과대학 본초약리학교실) , 김소영 (대구한의대학교 한의과대학 본초약리학교실) , 최문열 (대구한의대학교 한의과대학 본초약리학교실) , 김미형 (대구한의대학교 한의과대학 본초약리학교실) , 고경아 (대구한의대학교 한의과대학 본초약리학교실) , 김미려 (대구한의대학교 한의과대학 본초약리학교실) , 서부일 (대구한의대학교 한의과대학 본초약리학교실)

Abstract ▼ AI-Helper

Objective : This study was conducted to investigate antidiabetic effects of chunggugjang with medicinal herbal complex (CJ) in streptozotocin (STZ)-induced animal models. Method : STZ (65 mg/kg) was injected intraperitoneally to induce diabetes. Then rats were divided into 5 groups ; NG (normal diet + 0.9% saline), COS (STZ +saline 5 mL/kg), COB (STZ + fermented soybean(100 mg/kg), CJ 100/200 (STZ+CJ(100 and 200 mg/kg), CJ 300/600 (STZ+CJ(300 and 600 mg/kg). 4 weeks later, oral glucose tolerance test (OGTT) was performed. After sacrificing rats, serum levels of blood urea nitrogen (BUN), creatinine, aspartate amino transferase (AST), alanine amino transferase (ALT), total cholesterol (TC), and triglyceride (TG) were measured and histological changes were observed. Result : Body weight change and food efficiency ratio (FER) were decreased in the CJ 300/600 group than in the COS group. But, there was no change in water intake. Serum levels of glucose, AST, ALT and BUN were lower in the CJ 300/600 group than in the COS group. Also, TG, TC, and creatinine were decreased in the CJ 300/600 group than in the COS group. According to OGTT, 120 minutes postprandial glucose levels were lower in the CJ 300/600 group than in the COS group. In addition, administration of CJ extracts restored histopathological damage. Conclusion : The results suggest that CJ can be used as a functional material for diabetes treatment as it has the effect to improve pathological symptoms in STZ-induced diabetic rats.

주제어

표/그림 (8)

표 Table 1. Analysis of general components in Chunggugjang (CJ) and boiled bean extract (COB).
그림 Fig. 1. The effect of CJ on fasting blood glucose in STZ-induced diabetic rats. NG : Normal group, COS : STZ + Salin 5 ml/kg, COB : STZ + boiled bean extract 100 mg/5 mL/kg, CJ 100/200 : STZ+CJ 100/200 mg/kg/5 mL, CJ 300/600 : STZ+CJ 300/600 mg/kg/ 5 mL. Data expressed as the mean±SD (n =6). ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs. the NG group ; *P < 0.05, **P < 0.01, and ***P < 0.001 vs. COS group ; ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001 vs. COB group.
표 Table 2. Effects of CJ on body weight gain, food intake, water intake and food intake ratio in STZ-induced diabetic rats.
표 Table 3. The effects of CJ on organ weights in STZ-induced diabetic rats.
표 Table 4. The effects of CJ on serum lipid profiles in STZ-induced diabetic rats.
그림 Fig. 2. Effect of CJ on OGTT in STZ-induced diabetic rats. NG : Normal group, COS : STZ + Salin 5 ml/kg, COB : STZ + boiled bean extract 100 mg/5 mL/kg, CJ 100/200 : STZ+CJ 100/200 mg/kg/5 mL, CJ 300/600 : STZ+CJ 300/600 mg/kg/ 5 mL. Data expressed as the mean±SD (n =6). ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs. the NG group ; *P < 0.05, **P < 0.01, and ***P < 0.001 vs. COS group ; ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001 vs. COB group.
표 Table 5. The effects of CJ on serum functional enzyme levels in STZ-induced diabetic rats.
그림 Fig. 3. Effects of CJ on the histomorphology of liver, kidney and pancreas in STZ-induced diabetic rats. NG : Normal group, COS : STZ + saline 5 mL/kg COB : STZ + boiled bean extract 100 mg/5 mL/kg, CJ 100/200 : STZ+CJ 100/200 mg/kg/5 mL, CJ 300/600 : STZ+CJ 300/600 mg/kg/5 mL. Data expressed as the mean±SD (n=6). H&E stain, ×200

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