[논문]Penicillium vietnamense sp. nov., the First Novel Marine Fungi Species Described from Vietnam with a Unique Conidiophore Structure and Molecular Phylogeny of Penicillium Section Charlesia

Nguyen, Van Duy; Pham, Thu Thuy

doi:10.1080/12298093.2022.2068750

Penicillium vietnamense sp. nov., the First Novel Marine Fungi Species Described from Vietnam with a Unique Conidiophore Structure and Molecular Phylogeny of Penicillium Section Charlesia 원문보기

Mycobiology, v.50 no.3, 2022년, pp.155 - 165

Nguyen, Van Duy (Institute of Biotechnology and Environment, Nha Trang University) , Pham, Thu Thuy (Institute of Biotechnology and Environment, Nha Trang University)

Abstract ▼ AI-Helper

Penicillium vietnamense sp. nov. was isolated from Nha Trang Bay, Vietnam in June 2017. It is phylogenetically distinct from the sister species of Penicillium section Charlesia series Indica based on multi-locus sequence typing results using internal transcribed spacer, large subunit ribosomal RNA, b-tubulin, calmodulin, and RNA polymerase II second largest subunit regions. It showed strong growth on Czapek yeast autolysate agar at 37 ℃, a strong acid production on Creatine sucrose agar, and produced short stipes, small vesicles, and subglobose to globose conidia delicately roughened with very short ridges. As the first novel marine fungi species described from Vietnam and discovered in a unique environment, the data could be significant for understanding the taxonomy and geographical distribution of marine fungi in tropical coastal systems such as Vietnam.

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표/그림 (8)

표 Table 1. Phylogenetic loci and PCR primers used in the present study.
표 Table 2. Details of the strains used in phylogenetic and identity analyses.
그림 Figure 1. Phylogenetic tree based on the neighbor-joining analysis of the ITS sequences for Penicillium species classified in the Charlesia section. Penicillium eremophilum as the most related to the Charlesia section was included as an outgroup. Bootstrap analysis was performed with 1000 replications with values of at least 50% indicated at the nodes. The bar indicates the number of substitutions per position. Each branch indicates taxon name, strain name and GenBank accession number sequentially. (T) indicates the type strain of the species. The isolate in this study is marked as (◯).
그림 Figure 2. Phylogenetic tree based on the neighbor-joining analysis of the combined ITS, BenA, CaM, and RPB2 dataset for Penicillium species classified in the Charlesia section. Penicillium eremophilum was added as the most related species to the Charlesia section. Aspergillus oryzae was included as an outgroup. Bootstrap analysis was performed with 1000 replications with values of at least 50% indicated at the nodes. The bar indicates the number of substitutions per position. Each branch indicates taxon name and strain name sequentially. (T) indicates the type strain of the species. The isolate in this study is marked as (◯).
표 Table 3. BLASTn search against the strain DW14M (VTCC 930029) compared with the closest relatives and type strains in Genbank based on the ITS, LSU, BenA, CaM, and RPB2 sequences.
그림 Figure 3. Characteristics of Penicillium vietnamense (VTCC 930029=DW14M) grown on different media. Colony on (A) CYA 25 °C; (B) CYA 30 °C; (C) CYA 37 °C; (D) MEA 25 °C; (E) YESA 25 °C; (F) CYAS 25 °C; (G) CZ 25 °C; (H) OA 25 °C; (I) CREA 25 °C; (J) PDA 25 °C (top: obverse, bottom: reverse). (K-M) Conidiophores; (N) Conidia (Scale bar = 10 lm in (K)-(N)).
그림 Figure 4. (A-D) Conidial morphology of Penicillium vietnamense (VTCC 930029=DW14M) using scanning electron microscopy. Scale bar = 10 μm in (A). Scale bar = 5 μm in (B). Scale bar = 4 μm in (C). Scale bar = 2 μm in (D).
표 Table 4. Morphological comparison between Penicillium vietnamense and its closest species.

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