[논문]Investigation of Antioxidant, Hypoglycemic and Anti-Obesity Effects of Euphorbia Resinifera L.

Benrahou, Kaoutar; El Guourrami, Otman; Mrabti, Hanae Naceiri; Cherrah, Yahia; My El Abbes, Faouzi

doi:10.3831/kpi.2022.25.3.242

Investigation of Antioxidant, Hypoglycemic and Anti-Obesity Effects of Euphorbia Resinifera L. 원문보기

Journal of pharmacopuncture, v.25 no.3, 2022년, pp.242 - 249

Benrahou, Kaoutar (Laboratory of Pharmacology and Toxicology, Bio Pharmaceutical and Toxicological Analyzes Research Team, Faculty of Medicine and Pharmacy, Mohammed V University) , El Guourrami, Otman (Laboratory of Analytical Chemistry and Bromatology, Faculty of Medicine and Pharmacy, Mohammed V University) , Mrabti, Hanae Naceiri (Laboratory of Pharmacology and Toxicology, Bio Pharmaceutical and Toxicological Analyzes Research Team, Faculty of Medicine and Pharmacy, Mohammed V University) , Cherrah, Yahia (Laboratory of Pharmacology and Toxicology, Bio Pharmaceutical and Toxicological Analyzes Research Team, Faculty of Medicine and Pharmacy, Mohammed V University) , My El Abbes, Faouzi (Laboratory of Pharmacology and Toxicology, Bio Pharmaceutical and Toxicological Analyzes Research Team, Faculty of Medicine and Pharmacy, Mohammed V University)

Abstract ▼ AI-Helper

Objectives: The aim of this work is to evaluate the in vitro antioxidant, hypoglycemic, and antiobesity effects of Euphorbia resinifera extracts and investigate the phenolic constituents and the toxicity of these extracts. Methods: Phytochemical screening was performed to detect polyphenols and flavonoids. Antioxidant activity was evaluated by four methods (DPPH, ABTS, H2O2, and xanthine oxidase inhibition). The hypoglycemic effect was determined by the inhibition of α-amylase and α-glucosidase enzymes in vitro and via a starch tolerance study in normal rats. The antiobesity effect was estimated by in vitro inhibition of lipase. Results: Phytochemical screening revealed that the ethanolic extract was rich in polyphenols (99 ± 0.56 mg GEA/g extract) and tannins (55.22 ± 0.17 mg RE/g extract). Moreover, this extract showed higher antioxidant activity in different tests: the DPPH assay (IC50 = 53.81 ± 1.83 ㎍/mL), ABTS assay (111.4 ± 2.64 mg TE/g extract), H2O2 (IC50 = 98.15 ± 0.68 ㎍/mL), and xanthine oxidase (IC50 = 10.26 ± 0.6 ㎍/mL). With respect to hypoglycemic effect, the aqueous and ethanolic extracts showed IC50 values of 119.7 ± 2.15 ㎍/mL and 102 ± 3.63 ㎍/mL for α-amylase and 121.4 ± 1.88 and 56.6 ± 1.12 ㎍/mL for α-glucosidase, respectively, and the extracts lowered blood glucose levels in normal starch-loaded rats. Additionally, lipase inhibition was observed with aqueous (IC50 = 25.3 ± 1.53 ㎍/mL) and ethanolic (IC50 = 13.7 ± 3.03 ㎍/mL) extracts. Conclusion: These findings show the antioxidant, hypoglycemic, and hyperlipidemic effects of E. resinifera extracts, which should be investigated further to validate their medicinal uses and their pharmaceutical applications.

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표/그림 (5)

표 Table 1. Total phenols and flavonoids content of E. resinifera
표 Table 2. Antioxidant activity by DPPH, ABTS, H2O2 and xanthine oxidase (XO) methods of E. resinifera extracts
그림 Figure 1. Effect of Euphorbia resinifera on blood glucose after starch loading in normal rats (A) and with presentation in the area under curve (B). Values are means ± SEM (n = 5). Ns, not significant to the normal controls; ERA, aqueous extract of E. resinifera; ERE, ethanolic extract of E. resinifera; AUC, area under the curve.
표 Table 3. IC₅₀ values of E. resinifera extract on α-amylase, α-glucosidase and lipase inhibition
그림 Figure 2. HRMS mass spectrum obtained in negative mode for Saponarin in aqueous extract.

참고문헌 (40)

1 Noor ZI Ahmed D Rehman HM Qamar MT Froeyen M Ahmad S 2019 In vitro antidiabetic, anti-obesity and antioxidant analysis of Ocimum basilicum aerial biomass and in silico molecular docking simulations with alpha-amylase and lipase enzymes Biology (Basel) 8 4 92 10.3390/biology8040092 31817095

상세보기
2 Ajiboye BO Ojo OA Okesola MA Akinyemi AJ Talabi JY Idowu OT 2018 In vitro antioxidant activities and inhibitory effects of phenolic extract of Senecio biafrae (Oliv and Hiern) against key enzymes linked with type II diabetes mellitus and Alzheimer's disease Food Sci Nutr 6 7 1803 10 10.1002/fsn3.749 30349669

상세보기
3 Balasubramaniam V Mustar S Mustafa Khalid N Abd Rashed A Mohd Noh MF Wilcox MD 2013 Inhibitory activities of three Malaysian edible seaweeds on lipase and α-amylase J Appl Phycol 25 5 1405 12 10.1007/s10811-012-9964-4

상세보기
4 Worsztynowicz P Napierała M Białas W Grajek W Olkowicz M 2014 Pancreatic α-amylase and lipase inhibitory activity of polyphenolic compounds present in the extract of black chokeberry (Aronia melanocarpa L.) Process Biochem 49 9 1457 63 10.1016/j.procbio.2014.06.002

상세보기
5 Oboh G Akinyemi AJ Ademiluyi AO Adefegha SA 2010 Inhibitory effects of aqueous extracts of two varieties of ginger on some key enzymes linked to type-2 diabetes in vitro J Food Nutr Res 49 1 14 20
6 Poongothai K Ponmurugan P Ahmed KS Kumar BS Sheriff SA 2011 Antihyperglycemic and antioxidant effects of Solanum xanthocarpum leaves (field grown & in vitro raised) extracts on alloxan induced diabetic rats Asian Pac J Trop Med 4 10 778 85 10.1016/S1995-7645(11)60193-4 22014732

상세보기
7 Nair SS Kavrekar V Mishra A 2013 In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts Eur J Exp Biol 3 1 128 32
8 Wang S Liang H Zhao Y Wang G Yao H Kasimu R 2016 New triterpenoids from the latex of Euphorbia resinifera Berg Fitoterapia 108 33 40 10.1016/j.fitote.2015.11.009 26586618

상세보기
9 Boutoub O El-Guendouz S Estevinho LM Paula VB Aazza S El Ghadraoui L 2021 Antioxidant activity and enzyme inhibitory potential of Euphorbia resinifera and E. officinarum honeys from Morocco and plant aqueous extracts Environ Sci Pollut Res Int 28 1 503 17 10.1007/s11356-020-10489-6 32815014

상세보기
10 Wang SY Li GY Zhang K Wang HY Liang HG Huang C 2019 New ingol-type diterpenes from the latex of Euphorbia resinifera J Asian Nat Prod Res 21 11 1075 82 10.1080/10286020.2018.1498084 30607997

상세보기
11 Spanos GA Wrolstad RE 1990 Influence of processing and storage on the phenolic composition of Thompson Seedless grape juice J Agric Food Chem 38 7 1565 71 10.1021/jf00097a030

상세보기
12 Dewanto V Wu X Liu RH 2002 Processed sweet corn has higher antioxidant activity J Agric Food Chem 50 17 4959 64 10.1021/jf0255937 12166989

상세보기
13 Huang B Ke H He J Ban X Zeng H Wang Y 2011 Extracts of Halenia elliptica exhibit antioxidant properties in vitro and in vivo Food Chem Toxicol 49 1 185 90 10.1016/j.fct.2010.10.015 20971152

상세보기
14 Tuberoso CI Boban M Bifulco E Budimir D Pirisi FM 2013 Antioxidant capacity and vasodilatory properties of Mediterranean food: the case of Cannonau wine, myrtle berries liqueur and strawberry-tree honey Food Chem 140 4 686 91 10.1016/j.foodchem.2012.09.071 23692754

상세보기
15 Muruhan S Selvaraj S Viswanathan PK 2013 In vitro antioxidant activities of Solanum surattense leaf extract Asian Pac J Trop Biomed 3 1 28 34 10.1016/S2221-1691(13)60019-2 23570013

상세보기
16 Umamaheswari M AsokKumar K Somasundaram A Sivashanmugam T Subhadradevi V Ravi TK 2007 Xanthine oxidase inhibitory activity of some Indian medical plants J Ethnopharmacol 109 3 547 51 10.1016/j.jep.2006.08.020 17014977

상세보기
17 Chakrabarti R Singh B Prakrith VN Vanchhawng L Thirumurugan K 2014 Screening of nine herbal plants for in vitro α-amylase inhibition Asian J Pharm Clin Res 7 4 84 9
18 Kee KT Koh M Oong LX Ng K 2013 Screening culinary herbs for antioxidant and α-glucosidase inhibitory activities Int J Food Sci Technol 48 9 1884 91 10.1111/ijfs.12166

상세보기
19 Hu B Cui F Yin F Zeng X Sun Y Li Y 2015 Caffeoylquinic acids competitively inhibit pancreatic lipase through binding to the catalytic triad Int J Biol Macromol 80 529 35 10.1016/j.ijbiomac.2015.07.031 26193679

상세보기
20 Beejmohun V Peytavy-Izard M Mignon C Muscente-Paque D Deplanque X Ripoll C 2014 Acute effect of Ceylon cinnamon extract on postprandial glycemia: alpha-amylase inhibition, starch tolerance test in rats, and randomized crossover clinical trial in healthy volunteers BMC Complement Altern Med 14 351 10.1186/1472-6882-14-351 25249234

상세보기
21 Botham PA 2004 Acute systemic toxicity--prospects for tiered testing strategies Toxicol In Vitro 18 2 227 30 10.1016/S0887-2333(03)00143-7 14757114

상세보기
22 Farah H Ech-chahad A Lamiri A 2014 Antioxidant, antimicrobial and phytochemical investigations of polar extracts of Euphorbia resinifera Beg., roots, stems and flowers Am J Adv Drug Deliv 2 6 776 85
23 Peter EL Nagendrappa PB Ajayi CO Sesaazi CD 2021 Total polyphenols and antihyperglycemic activity of aqueous fruits extract of Abelmoschus esculentus: modeling and optimization of extraction conditions PLoS One 16 4 e0250405 10.1371/journal.pone.0250405 33861803

상세보기
24 Dudonné S Vitrac X Coutière P Woillez M Mérillon JM 2009 Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays J Agric Food Chem 57 5 1768 74 10.1021/jf803011r 19199445

상세보기
25 Boulfia M Lamchouri F Senhaji S Lachkar N Bouabid K Toufik H 2021 Mineral content, chemical analysis, In Vitro antidiabetic and antioxidant activities, and antibacterial power of aqueous and organic extracts of Moroccan Leopoldia comosa (L.) Parl. Bulbs Evid Based Complement Alternat Med 2021 9932291 10.1155/2021/9932291 34335845

상세보기
26 Hanane F Abdellah EC Abdeslam L 2014 In vitro antioxidant and antibacterial activity of the root extract of Euphorbia resinifera J Pharmacogn Phytochem 2 5 161 3
27 Sayah K Marmouzi I Naceiri Mrabti H Cherrah Y Faouzi ME 2017 Antioxidant activity and inhibitory potential of Cistus salviifolius (L.) and Cistus monspeliensis (L.) aerial parts extracts against key enzymes linked to hyperglycemia Biomed Res Int 2017 2789482 10.1155/2017/2789482 28116307

상세보기
28 Ameer K Shahbaz HM Kwon JH 2017 Green extraction methods for polyphenols from plant matrices and their byproducts: a review Compr Rev Food Sci Food Saf 16 2 295 315 10.1111/1541-4337.12253 33371540

상세보기
29 Birari RB Bhutani KK 2007 Pancreatic lipase inhibitors from natural sources: unexplored potential Drug Discov Today 12 19-20 879 89 10.1016/j.drudis.2007.07.024 17933690

상세보기
30 Singh U Singh S Kochhar A 2012 Therapeutic potential of antidiabetic nutraceuticals Phytopharmacology 2 1 144 69
31 Chater PI Wilcox M Cherry P Herford A Mustar S Wheater H 2016 Inhibitory activity of extracts of Hebridean brown seaweeds on lipase activity J Appl Phycol 28 1303 13 10.1007/s10811-015-0619-0 27057089

상세보기
32 Wang SY Huang C Sun RK Lu LN Liang HG Gao L 2019 New tirucallane triterpenoids from the dried latex of Euphorbia resinifera Phytochem Lett 29 220 4 10.1016/j.phytol.2018.12.012

상세보기
33 Gutiérrez-Del-Río I López-Ibáñez S Magadán-Corpas P Fernández-Calleja L Pérez-Valero Á Tuñón-Granda M 2021 Terpenoids and polyphenols as natural antioxidant agents in food preservation Antioxidants (Basel) 10 8 1264 10.3390/antiox10081264 34439512

상세보기
34 Chen YZ Zhang BW Yang J Zou CS Li T Zhang GC 2021 Detoxification, antioxidant, and digestive enzyme activities and gene expression analysis of Lymantria dispar larvae under carvacrol J Asia Pac Entomol 24 1 208 16 10.1016/j.aspen.2020.12.014

상세보기
35 Yang W Chen X Li Y Guo S Wang Z Yu X 2020 Advances in pharmacological activities of terpenoids Nat Prod Commun doi: 10.1177/1934578X20903555. [Epub ahead of print] 10.1177/1934578X20903555

상세보기
36 Ćavar Zeljković S Šišková J Komzáková K De Diego N Kaffková K Tarkowski P 2021 Phenolic compounds and biological activity of selected Mentha species Plants (Basel) 10 3 550 10.3390/plants10030550 33804017

상세보기
37 Fettach S Mrabti HN Sayah K Bouyahya A Salhi N Cherrah Y 2019 Phenolic content, acute toxicity of Ajuga iva extracts and assessment of their antioxidant and carbohydrate digestive enzyme inhibitory effects South Afr J Bot 125 381 5 10.1016/j.sajb.2019.08.010

상세보기
38 Naceiri Mrabti H Marmouzi I Sayah K Chemlal L El Ouadi Y Elmsellem H 2017 Arbutus unedo L aqueous extract is associated with in vitro and in vivo antioxidant activity J Mater Environ Sci 8 1 217 24
39 Simeonova R Vitcheva V Krasteva I Zdraveva P Konstantinov S Ionkova I 2016 Antidiabetic and antioxidant effects of saponarin from Gypsophila trichotoma on streptozotocin-induced diabetic normotensive and hypertensive rats Phytomedicine 23 5 483 90 10.1016/j.phymed.2016.02.024 27064007

상세보기
40 Sengupta S Mukherjee A Goswami R Basu S 2009 Hypoglycemic activity of the antioxidant saponarin, characterized as alpha-glucosidase inhibitor present in Tinospora cordifolia J Enzyme Inhib Med Chem 24 3 684 90 10.1080/14756360802333075 18951283

상세보기

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