[논문]무화과식초를 이용한 유제품의 개발과 인간 대장세포주에 미치는 영향

허지혜

doi:10.15324/kjcls.2022.54.3.217

무화과식초를 이용한 유제품의 개발과 인간 대장세포주에 미치는 영향
Development of Dairy Products Using Ficus carica Vinegar and the Effects on the Caco-2 Cell Line 원문보기

Korean journal of clinical laboratory science : KJCLS = 대한임상검사과학회지, v.54 no.3, 2022년, pp.217 - 223

허지혜 (동강대학교 임상병리학과)

초록
AI-Helper

다양한 건강기능식품 중 프로바이오틱스는 전 세계적으로 가장 방대한 시장을 이루고 있으며, 장 건강이 우리 몸의 전반적인 면역작용을 조절하며 질병을 예방하고, 나아가 치료에 도움을 줄 수 있다는 연구결과에 따라 지속적으로 프로바이오틱스에 대한 관심이 증가하고 있다. 본 연구에서는 무화과식초가 가진 항균능을 이용하여 유제품과 드레싱 소스를 개발하여 그 선호도를 조사하고 대장세포에 미치는 영향을 분석하고자 하였다. 일반우유에 5% 무화과식초를 첨가하였을 때 마시는 요거트와 리코타치즈에 대한 가장 만족도가 높았다. 제조된 유제품에서 분리한 균은 Leuconostoc lactis이며 1.0×10⁷~1.0×10⁸ CFU/mL 이상의 세균수가 측정되어 식품의 기준 및 규격의 기준치를 만족하였다. 인체의 소장 상피세포와 흡사하게 증식되는 Caco-2 세포주에서 세포생존율을 관찰한 결과, 유청을 10% 농도로 처리하였을 때 세포생존율이 19% 가량 유의적으로 증가하였다. 농도별로 유청을 처리하였을 때 최대 58%의 항산화능을 보였다. 또한 유청에서 분리된 Leuconostoc lactis는 우수한 부착능이 관찰됨에 따라 장건강에 도움을 주는 유제품임을 확인하였다.

Abstract ▼ AI-Helper

Among various health functional foods, probiotics constitute the largest market. The interest in probiotics is increasing continuously according to the research results that gut health can control the immune function of the body, prevent diseases, and assist in treatment. In this study, dairy products and dressing sauces were developed using Ficus carica vinegar (FV), and their effects on colon cells were analyzed. When 5% FV was added to regular milk, the satisfaction with the resulting yogurt and ricotta cheese was high. The dairy product was Leuconostoc lactis, and the number of bacteria was more than 1.0×107~1.0×108 CFU/mL. The product satisfied the health food standards as probiotics. An examination of the cell viability of Caco-2 cells, which proliferate similarly to human intestinal epithelial cells, revealed an approximately 19% increase in the proliferation rate when treated with whey at 10%. An antioxidant activity of up to 58% was recorded when the cells were treated with whey at various concentrations. In addition, excellent adhesion was observed for L.latis isolated from whey. This study confirmed that dairy products made using traditionally fermented FV assist intestinal health effectively as the microbiome.

주제어

표/그림 (7)

표 Table 1. Viable cells (log CFU/mL) counts on drink yogurt from regular milk or sterilized milk with concentration of FV
표 Table 2. Viable cells (log CFU/mL) counts on ricotta cheese from regular milk or sterilized milk with concentration of FV
표 Table 3. Viable cells (log CFU/mL) counts on dressing sauce with concentration of FV
표 Table 4. Sequence similarity of bacteria from drink yogurt from regular milk with 5% FV
그림 Figure 1. Percent of proliferation rate by concentration of whey from seperated with ricotta cheese at Caco-2 cell line. Data are presented as mean±SD (N=3). *P<0.05; P-values were calculated by paired Student t test. Abbreviation: C, control.
그림 Figure 2. Superoxide dismutase activity by concentration of whey from seperated with ricotta cheese at Caco-2 cell line. Each bar showed the mean±SD (N=3). P-values were calculated by paired Student t test. P=NS. Abbreviation: A, 100 μg/mL arbutin.
그림 Figure 3. Adhesion ability of Leuconostoc lactis from whey from seperated with ricotta cheese at Caco-2 cell line. Each bar showed the mean±SD (N=3). P-values were calculated by paired Student t test. P=NS.

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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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