[논문]아로니아 첨가 배지 내 느타리(Pleurotus ostreatus) 균사체 생장 및 항산화 효과

최성주; 편하영; 박태민; 박윤진; 오태석; 조용구; 장명준

doi:10.14480/jm.2022.20.3.102

아로니아 첨가 배지 내 느타리(Pleurotus ostreatus) 균사체 생장 및 항산화 효과
Mycelial growth and antioxidant effects of Pleurotus ostreatus in medium supplemented with Aronia berries 원문보기

Journal of mushrooms = 한국버섯학회지, v.20 no.3, 2022년, pp.102 - 106

최성주 (공주대학교 식물자원학과) , 편하영 (공주대학교 식물자원학과) , 박태민 (공주대학교 식물자원학과) , 박윤진 (두과녹비자원연구센터) , 오태석 (공주대학교 식물자원학과) , 조용구 (공주대학교 식물자원학과) , 장명준 (공주대학교 식물자원학과)

초록
AI-Helper

아로니아 첨가 시 생육 일자에 따른 느타리 균사체 생장량 및 pH 양상을 확인하였다. 무처리 대비 아로니아 첨가 시 균사체 직경과 건물중이 증가하는 경향을 보였으며, pH의 경우 아로니아 처리 시 배지의 농도가 낮아지는 것을 확인할 수 있었다. 아로니아 10% 첨가 배지에서 배양한 버섯 균사체와 일반 배지에서 배양한 버섯 균사체의 DPPH 라디칼 소거 활성에서 아로니아 첨가 배지 내 느타리 균사체는 61.81%, 무첨가 배지 내 느타리 균사체는 49.65%로 약 12%의 라디칼 소거 활성 차이를 보였으며, ABTS 라디칼 소거 활성은 아로니아 첨가 배지 내 느타리 균사체 59.83%, 무첨가 배지 내 느타리 균사체 52.66%로 나타나 7.17%의 라디칼 소거 활성 차이를 보였다. 총 폴리페놀 함량의 경우 아로니아 첨가 배지 내 생육 균사체 6.54 mg GAE/g, 무첨가 배지 내 생육 균사체 5.77 mg GAE/g로 0.77 mg GAE/g의 차이를 나타냈다. 생육 배지에 아로니아를 첨가함에 따라 느타리 균사체의 총 폴리페놀 함량이 많아지며 ABTS, DPPH에 의한 항산화 효과가 높아지는 경향을 나타냈다.

Abstract ▼ AI-Helper

This study was conducted to investigate the growth and antioxidant activity of Pleurotus ostreatus mycelia grown in medium supplemented with Aronia. The diameter and dry weight of the mycelia were increased in the medium supplemented with Aronia compared with the untreated medium. The total polyphenol content of mycelia grown in medium supplemented with Aronia and untreated medium was 6.54 mg GAE/g and 5.77 mg GAE/g, respectively. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of mycelia grown in medium supplemented with Aronia and untreated medium was 61.81% and 49.65%, respectively. Moreover, the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity of mycelia grown in medium supplemented with Aronia and untreated medium was 59.83% and 52.66%, respectively. These results confirmed that P. ostreatus mycelial growth and antioxidant activity were increased when Aronia was added to the culture medium.

주제어

표/그림 (7)

그림 Fig. 1. Morphology of P. ostreatus mycelium growth by addition of aronia extrats concentration. A, Control (PDA); B, aronia 1%; C, aronia 3%; D, aronia 5%; E, aronia 10%. Incubation period: 7 days. Temperature: 25 °C.
그림 Fig. 2. Changes in medium pH according to the aronia concentration
표 Table 1. Growth of mycelium in solid medium added to aronia extracts (Unit: cm)
표 Table 2. Growth of mycelium in liquid medium added to aronia extracts (Unit: mg)
그림 Fig. 3. DPPH radical scavenging activity in P. ostreatus extracts. A, PDB medium after growth of mycelium; B, PDB medium added to aronia 10% after growth of P. ostreatus mycelium; C, P. ostreatus mycelium in PDB medium; D, P. ostreatus mycelium in medium added to 10% aronia. White bar indicates a positive control; Different letters are significantly different by LSD multiple range test (p<0.05).
그림 Fig. 4. ABTS radical scavenging activity in P. ostreatus extracts. A, PDB medium after growth of mycelium; B, PDB medium added to aronia 10% after growth of P. ostreatus mycelium; C, P. ostreatus mycelium in PDB medium; D, P. ostreatus mycelium in medium added to 10% aronia. White bar indicates a positive control; Different letters are significantly different by LSD multiple range test (p<0.05).
표 Table 3. Total polyphenol contents in P. ostreatus extracts

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