[논문]Pharmacological potential of Cordyceps militaris with enhanced Cordycepin production for anti-inflammatory and tumor cell anti-proliferative applications

Ha, Si-Young; Jung, Ji-Young; Yang, Jae-Kyung

doi:10.14480/jm.2022.20.3.93

Pharmacological potential of Cordyceps militaris with enhanced Cordycepin production for anti-inflammatory and tumor cell anti-proliferative applications 원문보기

Journal of mushrooms = 한국버섯학회지, v.20 no.3, 2022년, pp.93 - 101

Ha, Si-Young (Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University) , Jung, Ji-Young (Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University) , Yang, Jae-Kyung (Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University)

Abstract ▼ AI-Helper

Cordyceps militaris mycelium extracts containing high amounts of cordycepin were evaluated in vitro for their anti-inflammatory and tumor cell growth-inhibitory activities. All extracts dose dependently inhibited the increased production of inflammatory mediators including reactive oxygen species (ROS), nitric oxide (NO), and 𝛽-hexosaminidase in lipopolysaccharide (LPS)-stimulated inflammatory cells. All extracts were evaluated for anti-proliferative activity against normal RBL-2H3 cells and diverse types of cancer cell lines, including HCT, MC5-7, U-87MG, AGS, and A549 cells. The extract showed the strongest growth inhibition (IC50 = 28.13 ㎍/mL) relative to vehicle-treated control cells against fibrosarcoma (MC5-7). We have demonstrated anti-inflammatory activity of C. militaris via inhibition of NO, ROS production, and 𝛽-hexosaminidase release in activated cells. C. militaris mycelium extract was also evaluated mechanistically and found to exert six types of anti-cancer activity, confirming its pharmacological potential. Our study suggests C. militaris use as a potential source of anti-inflammatory and anti-cancer agents. C. militaris may also be considered a functional food.

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표/그림 (8)

그림 Fig. 1. Effect of extraction condition on the extraction content of cordycepin from C. militaris.
그림 Fig. 2. The effects of extract obtained from C. militaris on the cell viability of RBL-2H3 mast cell.
그림 Fig. 3. The effects of extract obtained C. militaris on β- hexosaminidase induced by DNP-BSA from RBL-2H3 cells. +++ indicates a significant difference (p<0.001) compared with the DNP-IgE; ** indicates a significant difference (p<0.01) compared with the DNP-BSA; *** indicates a significant difference (p<0.001) compared with the DNPBSA.
그림 Fig. 4. The effects of extract obtained from C. militaris on the ROS of RAW 264.7 cell. *** indicates a significant difference (p<0.001) compared with the LPS.
그림 Fig. 5. The effects of extract obtained from C. militaris on the NO of RAW 264.7 cell. *** indicates a significant difference (p<0.001) compared with the LPS.
그림 Fig. 6. The effects of extract obtained from C. militaris on the five types cancer cell lines (HCT (colorectal cancer), MC5-7 (Fibrosarcoma cancer), U-87MG (brain tumor), AGS (stomach cancer) and A549 (lung cancer)). * p<0.05, ** p<0.01 and *** p<0.001 indicates a significant difference compared with the untreated sample (0 ppm).
표 Table 1. IC50 values of extract against HCT, MC5-7, U-87MG, AGS and A549 cells.
그림 Fig. 7. Cell morphology of MC5-7 using a phase-contrast microscope

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