[논문]RAW 264.7 에서 MAPKs 경로를 통한 Rumex acetosa L.의 항염증, 항산화 효과

성진영; 김용민

doi:10.15230/scsk.2022.48.3.213

RAW 264.7 에서 MAPKs 경로를 통한 Rumex acetosa L.의 항염증, 항산화 효과
Anti-inflammatory and Anti-oxidative Effects of Rumex acetosa L. in RAW 264.7 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.48 no.3, 2022년, pp.213 - 223

초록
AI-Helper

본 연구에서는 과도한 산화 스트레스에 따른 각종 염증 질환과 피부 노화를 예방하기 위해 수영 지상부(aerial parts of Rumex acetosa L.) 추출물의 항염증, 항산화 효과를 확인하였다. ABTS assay를 시행한 결과 농도 의존적으로 radical 소거능이 증가함을 확인하였다. 활성산소종(reactive oxygen species, ROS) 억제능은 DCF-DA assay를 통하여 확인하였고, 농도 의존적인 ROS 생성억제를 확인하였다. ROS에 따른 세포 핵 손상 억제 효과는 DAPI staining을 통하여 확인하였다. 또한 iNOS, COX-2의 mRNA 발현 수준은 qPCR을 통하여 농도 의존적으로 억제함을 확인하였다. iNOS, COX-2의 단백질 수준을 western blotting을 통해 확인한 결과, iNOS는 모든 농도에서 유의하게 감소하였으며, COX-2는 800 ㎍/mL에서 유의하게 감소하였다. iNOS에 의해 생성된 NO의 생성 억제 효과는 NO assay로 확인하였으며 농도 의존적으로 NO가 감소하였다. 또한, MAPKs 신호전달 경로 중 ERK와 JNK의 인산화를 억제하였다. 따라서 Rumex acetosa L.은 MAPKs 경로를 통한 항염증 효과와 항산화 효과를 보여 항염증 및 항산화 화장품 원료로 사용될 수 있는 가능성이 있다.

Abstract ▼ AI-Helper

In this study, the anti-inflammatory and antioxidant effects of aerial parts of Rumex acetosa L. extract were confirmed to prevent various inflammatory diseases and skin aging caused by excessive oxidative stress. As a result of ABTS assay, it was confirmed that the radical scavenging ability increased in a concentration-dependent manner. ROS inhibitory ability was confirmed through DCF-DA assay, and concentration-dependent inhibition of ROS production was confirmed. The effect of inhibiting cell nuclear damage according to ROS was confirmed through DAPI staining. In addition, it was confirmed that the mRNA expression levels of iNOS and COX-2 were inhibited in a concentration-dependent manner through qPCR. As a result of confirming the protein levels of iNOS and COX-2 by western blotting, iNOS was significantly decreased at all concentrations, and COX-2 was significantly decreased at 800 ㎍/mL. The inhibitory effect on the production of NO generated by iNOS was confirmed by NO assay, and NO was decreased in a concentration-dependent manner. In addition, phosphorylation of ERK and JNK in the MAPKs signaling pathway were inhibited. Therefore, Rumex acetosa L. has the potential to be used as an anti-inflammatory and antioxidant cosmetic raw material by showing anti-inflammatory and antioxidant effects through the MAPKs pathway.

주제어

표/그림 (9)

표 Table 1. Gene Name and Assay ID Number in qPCR Analysis
그림 Figure 1. The effect of R. acetosa L . on A B TS radical scavenging test. Control; untreated group. Positive control; Ascorbic acid (Aa) 200 μM. 100, 200, 400 and 800 μg/mL; treated with various concentrations of R. acetosa L. The IC₅₀ value is 500 μg/mL. Indicates a significant difference from the control(p < 0.05).
그림 Figure 2. The effect of R. acetosa L. on cell viability in RAW 264.7 macrophage cells. Control; untreated group. 100, 200, 400, and 800 μg/mL; treated with various concentrations of R. acetosa L.
표 Table 2. Antibody Name and Assay ID Number in Western Blotting Analysis
그림 Figure 3. The effects of R. acetosa L. on DCF-DA in LPS-induced RAW 264.7 macrophage cells. (A) Control; untreated group. LPS; treated with LPS 1 μg/mL. 100, 200, 400 and 800 μg/mL: treated with various concentrations of R. acetosa L. with LPS (1 μg/mL). (B) Analysis data using image J. The IC₅₀ value is 345 μg/mL. Indicates a significant difference from the LPS (p < 0.05).
그림 Figure 4. The effects of R. acetosa L. on DAPI in LPS-induced RAW 264.7 macrophage cells. (A) Control; untreated group. LPS; treated with LPS 1 μg/mL. 100, 200, 400 and 800 μg/mL: treated with various concentrations of R. acetosa L. with LPS (1 μg/mL). (B) Analysis data using image J. The IC₅₀ value is 78 μg/mL. Indicates a significant difference from the LPS (p < 0.05).
그림 Figure 5. The effects of R. acetosa L. on LPS-induced iNOS, COX-2 mRNA expressions in RAW 264.7 macrophage cells. Control; untreated group. LPS; treated with LPS 1 μg/mL. 100, 200, 400 and 800 μg/mL; treated with various concentrations of R. acetosa L. with LPS (1 μg/mL). The IC₅₀ value of iNOS was 230 μg/mL, and the IC₅₀ value of COX-2 was 369 μg/mL. Indicates a significant difference from the LPS(p < 0.05).
그림 Figure 6. The effects of R. acetosa L. on NO production in LPS-induced RAW 264.7 macrophage cells. Control; untreated group. LPS; treated with LPS 1 μg/mL. 100, 200, 400 and 800 μg/mL; treated with various concentrations of R. acetosa L. with LPS (1 μg/mL). The IC₅₀ value is 1 mg/mL. Indicates a significant difference from the LPS(p < 0.05).
그림 Figure 7. The effects of R. acetosa L. on iNOS, COX-2, MEK, ERK, JNK, p38 protein expression in LPS-induced RAW 264.7 macrophage cells by western blotting. Control; untreated group. LPS; treated with LPS 1 μg/mL. 100, 200, 400 and 800 μg/mL; treated with various concentrations of R. acetosa L. with LPS (1 μg/mL). The IC₅₀ values of iNOS, COX-2, MEK, ERK, and JNK were 71 μg/mL, 2 mg/mL, 91 μg/mL, 88 μg/mL, 183 μg/mL, respectively. Indicates a significant difference from the LPS (p < 0.05).

참고문헌 (26)

M. Gasparrini, S. Afrin, T. Forbes-Hernandez, D. Cianciosi, P. Reboredo-rodriguez, A. Amici, M. Battino, and F. Giampieri, Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages. Part 2: control of oxidative stress induced damage, increase of antioxidant enzyme activities and attenuation of inflammation, Food. Chem. Toxicol. 120, 578 (2018).

상세보기
D. W. Kim, H. J. Yun, J. Y. Heo, T. H. Kim, H. J. Cho, and S. D. Park, Anti-oxidative and antiinflammatory effect of Do-Ki-Tang methanol extract in mouse macrophage cells, Kor. J. Herbology, 25(4), 103 (2010).

원문보기 상세보기
C. Peyssonnaux and A. Eychene, The Raf/MEK/ERK pathway: new concepts of activation, Biol. Cell, 93(1-2), 53 (2001).

상세보기
S. J. Kim, Master's Thesis Dissertation, Semyung Univ., Jecheon, Korea (2020).
J . H. Ryu, J . H. An, Y. K. Woo, and H. J . Cho, The anti-inflammation effects of A.C.C. extracts on the LPS-induced RAW 264.7 cell, JKAIS, 18(12), 503 (2017).
M. G. Park and Y. W. Kang, Anti-oxidant and antiinflammatory activities of various organ extracts from Trichosanthes kirilowii maxim, Korea J. of Pharmacogn, 47(4), 327 (2016).
D. H. Yoo and J. Y. Lee, Anti-inflammatory activities verification of Ambrosia trifida L. extract in RAW 264.7 cells, Microbiol. Biotechnol. Lett., 48(1), 79 (2020).
H. K. Kim, The effects of anti-inflammatory activities and active fractions analysis of ethanol extract from red rose petals, JCCT, 6(2), 543 (2020).
S. H. Seo and M. O. Choi, Protectvie effects of Lonicerae japonicae flos against hydrogen peroxidaseinduced oxidative stress on human keratinocyte, HaCaT cells, Kor. J. Herbology, 28(4), 57 (2013).
H. S. Kim, Y. M. Lee, M. A. Yoo, J. W. Lee, S. H. Ryu, and K. W. Kim, Ofloxacin inhibits hypoxia/ hypoglycemia-induced apoptosis in bovine aortic endothelial cells, JCP, 6(3), 155 (2001).
H. J . Son, H. K. Park, S. A. Lee, E. Y. J ung, and C. W. Jung, The characteristics of microbial community for biological activated carbon in water treatment plant, J. Korean Soc. Environ. Eng., 27(12), 1311 (2005).
J. Schmuch, S. Becker, S. Brandt, G. Lohr, F. Hermann, T. J. Schmidt, T. Beikler, and A. Hensel, Extract from Rumex acetosa L. for prophylaxis of periodontitis: inhibition of bacterial in vitro adhesion and of gingipains of Porphyromonas gingivalis by epicatechin-3-O-(4β, 8)-epicatechin-3'-O-gallate (procyanidin-B2-Di-gallate), PLoS ONE, 10(3), e0120130 (2015).

상세보기
N. J . Lee, J . H. Choi, B. S. Koo, S. Y. Ryu, Y. H. Han, S. I. Lee, and D. U. Lee, Antimutagenicity and cytotoxicity of the constituents from the aerial parts of Rumex acetosa, J-STAGE, 28(11), 2158 (2005).
Y. Y. Sun, X. H. Su, J. Y. Jin, Z. Q. Zhou, S. S. Sun, J . F. Wen, D. G. Kang, H. S. Lee, K. W. Cho, and S. N. Jin, Rumex acetosa L. induces vasorelaxation in rat aorta via activation of PI3-kinase/Akt- and Ca2+-eNos-NO signaling in endothelial cells, J. Physiol. Pharmacol., 66(6), 907 (2015).
S. E. Lee J . H. Lee, J . K. Kim, G. S. Kim, Y. O. Kim, J . S. Soe, J . H. Choi, E. S. Lee, H. J . Noh, and S. Y. Kim, Anti-inflammatory activity of medicinal plant extracts, KJMCS, 19(4) 217 (2011).
J. Bicker, F. Petereit, and A. Hensel, Proanthocyanidins and a phloroglucinol derivative from Rumex acetosa L., Fitoterapia, 80(8), 483 (2009).

상세보기
H. K. Kim, B. S. Cheon, Y. H. Kim, S. Y. Kim, and H. P. Kim, Effects of naturally occurring flavonoids on nitric oxide production in the macrophage cell line RAW 264.7 and their structure - activity relationships, Biochem. Pharmacol., 58(5), 759 (1999).

상세보기
S. Baek, J. H. Choi, S. H. Ko, Y. J. Lee, D. S. Cha, E. Y. Park, and Y. G. Kang, Antioxidant and antiinflammatory effect of Nardostachys chinensis in IFN-γ/LPS-stimulated peritoneal macrophage, JPPKM, 23(4), 853 (2009).
S. H. J eon, S. L. Oh, S. J . Kim, B. H. J eon, J . Y. Sung, and Y. M. Kim, Anti-oxidative effect of Chungsimyeonjaum (CSYJE) via Nrf2/HO-1 pathway activity in lipopolysaccharide (LPS) induced RAW 264.7 macrophages, J. Soc. Cosmet. Scientists Korea, 46(3), 253 (2020).

원문보기 상세보기
S. H. Hong, H. N. Lee, T. Y. Jeong, Y. H. Kim, H. S. Jeong, and J. S. Lee, Protective effect of Jujube water extract against UVB-induced skin photoaging in human skin fibroblasts, JKSFSN, 48(10), 1172 (2019).
J. J. Nam and Y. J. Kim, Fractionated Trapa japonica extracts inhibit ROS-induced skin inflammation in HaCaT keratinocytes, J. Soc. Cosmet. Scientists Korea, 41(1), 45 (2015).

원문보기 상세보기
J . S. Seo, T. H. Lee, S. M. Lee, S. E. Lee, N. S. Seung, and J. Y. Kim, Inhibitory effects of methanolic extracts of medicinal plants on nitric oxide production in activated macrophage RAW 264.7 Cells, KJMCS, 17(3), 173 (2009).
D. H. Jeong, B. K. Kang, K. B. W. R. Kim, M. J. Kim, and D. H. Ahn, Anti-inflammatory activity of Sargassum micracanthum water extract, J. Appl. B iol. Chem., 57(3), 227 (2014).
Crofford. LJ, COX-1 and COX-2 tissue expression: implications and predictions, J. Rheumatol., 49, 15 (1997).
D. J. Choi, G. S. Kim, B. R. Choi, Y. S. Lee, K. S. Han, D. S. Lee, and D. Y. Lee, Anti-inflammatory effect of a mixture of Astragalus membranaceus and Lithospermum erythrorhizon extracts by inhibition of MAPK and NF-κB signaling pathways in RAW264.7 cells, J. Appl. Biol. Chem., 63(4), 421 (2020).
H. K. Kim, Role of ERK/MAPK signalling pathway in anti-inflammatory effects of Ecklonia cava in activated human mast cell line-1 cells, Asian Pac. J. Trop. Med., 7(9), 703 (2014).

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증