[논문]진달래 뿌리 추출물의 알레르기 염증 억제 효과

장시성; 이대중; 송지훈; 박도휘; 전찬용; 황귀서

doi:10.22246/jikm.2022.43.1.68

진달래 뿌리 추출물의 알레르기 염증 억제 효과
Inhibitory Effect of Rhododendron Mucronulatum Root Extract on Allergic Inflammation 원문보기

대한한방내과학회지 = The journal of internal Korean medicine, v.43 no.1, 2022년, pp.68 - 78

장시성 (가천대학교 한의과대학) , 이대중 (가천대학교 한의과대학) , 송지훈 (제주 한의약 연구원) , 박도휘 (가천대학교 한의과대학) , 전찬용 (가천대학교 한의과대학) , 황귀서 (가천대학교 한의과대학)

Abstract ▼ AI-Helper

Objective: In this study, we investigated the protective effect of rhododendron mucronulatum extract (RME) on allergic reactions and inflammation. Methods: The effect of RME was determined using ELISA and RT-PCR in RBL-2H3 mast cells and RAW 264.7 macrophage cells. We determined cell viability, β-hexosaminidase release, and the synthesis of IL-4 and TNF-α in RBL-2H3 cells. In addition, we determined NO from RAW 264.7 and the gene expression of IL-1β, iNOS, IL-6, TNF-α, and IL-10. Results: RME inhibited β-hexosaminidase release and synthesis of IL-4 and TNF-α in RBL-2H3 by the anti-DNP IgE plus DNP-HSA stimulation. In addition, RME inhibited the production of NO and the gene expression of IL-1β, iNOS, IL-6, and TNF-α in LPS-stimulated RAW 264.7 cells. Conclusion: From these results, we concluded that RME possesses anti-allergic activity and anti-inflammatory activity due to the inhibition of mast cells and macrophage function.

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표/그림 (12)

그림 Fig. 1. DPPH radical scavenging activity of RME. 50 : 50 ug/ml of RME 100 : 100 ug/ml of RME 150 : 150 ug/ml of RME 200 : 200 ug/ml of RME
그림 Fig. 2. Effect of RME on viability of RBL-2H3 cells. C : normal D : 100 ug/ml of dexamethasone 12.5 : 12.5 ug/ml of RME
그림 Fig. 3. Effect of RME on granule release in HSA-treated RBL-2H3 mast cells. Fig. 3. Effect of RME on granule release in HSAtreated RBL-2H3 mast cells. RBL-2H3 cells were activated by HAS and measured β-hexosaminidase level in cell incubated for 6 hours with RME. N : normal C : HSA only 25 : HSA+25 ug/ml of RME 50 : HSA+50 ug/ml of RME 100 : SA+100 ug/ml of RME 200 : HSA+200 ug/ml of RME ** : p<0.01 vs N, ##: p<0.01
그림 Fig. 4. Effect of RME on IL-4 synthesis in HSAtreated RBL-2H3 mast cells. RBL-2H3 cells were activated by HAS and measured IL-4 level in medium incubated for 6 hours with RME. N : normal C : HSA only D : HSA+100ug/ml of dexamethasone 25 : HSA+25 ug/ml of RME 50 : HSA+50 ug/ml of RME 100 : HSA+100 ug/ml of RME ** : p<0.01 vs N, ##: p<0.01
그림 Fig. 5. Effect of RME on TNF-α synthesis in HSAtreated RBL-2H3 mast cells. RBL-2H3 cells were activated by HAS and measured TNF-α level in medium incubated for 6 hours with RME. N : normal C : HSA only 25 : HSA+25 ug/ml of RME 50 : HSA+50 ug/ml of RME 100 : HSA+100 ug/ml of RME ** : p<0.01 vs N, #: p<0.05 vs C
그림 Fig. 6. Effect of RME on LPS-induced NO synthesis in RAW 264.7 macrophage cells. N : normal C : LPS only 25 : LPS+25 ug/ml of RME 50 : LPS+50 ug/ml of RME 100 : LPS+100 ug/ml of RME 200 : LPS+200 ug/ml of RME ** : p<0.01 vs N, ##: p<0.01 vs C
그림 Fig. 7. Effect of RME on cell viability of RAW 264.7 cells. C : normal 12.5 : 12.5 ug/ml of RME 25 : 25 ug/ml of RME 50 : 50 ug/ml of RME 100 : 100 ug/ml of RME 200 : 200 ug/ml of RME
그림 Fig. 8. Effect of RME on LPS-induced IL-1β gene expression in Raw 264.7 cells. N : normal C : LPS only 50 : LPS+50 ug/ml of RME 100 : LPS+100 ug/ml of RME 200 : LPS+200 ug/ml of RME ** : p<0.01 vs N, ##: p<0.01 vs C
그림 Fig. 9. Effect of RME on LPS-induced iNOS gene expression in Raw 264.7 cells. N : normal C : LPS only 50 : LPS+50 ug/ml of RME 100 : LPS+100 ug/ml of RME 200 : LPS+200 ug/ml of RME ** : p<0.01 vs N, ##: p<0.01 vs C
그림 Fig. 10. Effect of RME on LPS-induced IL-6 expression in Raw 264.7 cells. N : normal C : LPS only 50 : LPS+50 ug/ml of RME 100 : LPS+100 ug/ml of RME 200 : LPS+200 ug/ml of RME ** : p<0.01 vs N, ##: p<0.01 vs C
그림 Fig. 11. Effect of RME on LPS-induced IL-10 expression in Raw 264.7 cells. N : normal C : LPS only 50 : LPS+50 ug/ml of RME 100 : LPS+100 ug/ml of RME 200 : LPS+200 ug/ml of RME ** : p<0.01 vs N
그림 Fig. 12. Effect of RME on LPS-induced TNFα expression in Raw 264.7 cells. N : normal C : LPS only 50 : LPS+50 ug/ml of RME 100 : LPS+100 ug/ml of RME 200 : LPS+200 ug/ml of RME ** : p<0.01 vs N, ##: p<0.01 vs C

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