[논문]엽록소 형광반응을 이용한 인삼 유전자원의 습해 스트레스 평가

지무근; 홍영기; 김선익; 박용찬; 이가순; 장원석; 권아름; 성봉재; 김미선; 조용구

doi:10.5010/jpb.2022.49.3.240

엽록소 형광반응을 이용한 인삼 유전자원의 습해 스트레스 평가
Evaluation of waterlogging tolerance using chlorophyll fluorescence reaction in the seedlings of Korean ginseng (Panax ginseng C. A. Meyer) accessions 원문보기

Journal of plant biotechnology = 식물생명공학회지, v.49 no.3, 2022년, pp.240 - 249

지무근 (충청남도농업기술원 인삼약초연구소) , 홍영기 (충청남도농업기술원 인삼약초연구소) , 김선익 (충청남도농업기술원 인삼약초연구소) , 박용찬 (충청남도농업기술원 인삼약초연구소) , 이가순 (충청남도농업기술원 인삼약초연구소) , 장원석 (충청남도농업기술원 인삼약초연구소) , 권아름 (충청남도농업기술원 인삼약초연구소) , 성봉재 (충청남도농업기술원 인삼약초연구소) , 김미선 (충북대학교 식물자원학과) , 조용구 (충북대학교 식물자원학과)

초록
AI-Helper

본 연구는 인삼 습해 피해 설정을 확립하고 유전자원을 대상으로 엽록소형광 기법을 이용하여 습해 스트레스 수준을 평가하기 위해 수행되었다. 습해 스트레스 평가는 2년생 묘삼 57개 유전자원 대상으로 25일간 지제부까지 담수처리를 하였다. 피해 정도에 따라 5개 등급으로 분류하여 저항성 유전자원 3개, 감수성 유전자원 3개를 선발하였다. 인삼 유전자원 57개의 지하부 생체중 및 엽록소 함량은 대부분 감소하였고 저항성 유전자원보다 감수성 유전자원의 감소율이 크게 나타났다. 5개의 엽록소형광 매개변수는 습해 스트레스에 노출되는 기간이 길어질수록 증감하는 경향을 보였으며, 특히, Fv/Fm값이 0.8보다 낮으면 광계II의 반응중심이 손상을 입은 상태로 감수성 유전자원의 경우 습해 스트레스에 노출될수록 감소하였다. 본 연구결과에서 유전자원 57개의 최대양자수율(Fv/Fm)은 습해 스트레스 하에서 차이를 보였으며, 습해 스트레스에 저항성을 가진 습해 저항성 유전자원과 감수성 유전자원 간의 차이를 확인할 수 있는 매개변수로 판단되어 인삼 유전자원에 대해 습해 스트레스 수준을 평가할 수 있는 도구로 활용 가능하다고 사료된다.

Abstract ▼ AI-Helper

Measuring chlorophyll fluorescence (CF) is a useful tool for assessing a plant's ability to tolerate abiotic stresses such as drought, waterlogging and high temperature. Korean ginseng is highly sensitive to water stress in paddy fields. To evaluate the possibility of non-destructively diagnosing waterlogging stress using chlorophyll fluorescence (CF) imaging techniques, we screened 57 ginseng accessions for waterlogging tolerance. To evaluate waterlogging tolerance among the 2-year-old Korean ginseng accessions, we treated ginseng plants with water stress for 25 days. The physiological disorder rate was characterized through visual assessment (an assigned score of 0-5). The physiological disorder rates of Geumjin, Geumsun and GS00-58 were lower than that of other accessions. In contrast, lines GS97-62, GS97-69 and GS98-1-5 were deemed susceptible. Root traits, chlorophyll content and the reduction rates decreased in most ginseng accessions. Further, these metrics were significantly lower in susceptible genotypes compared to resistant ones. All CF parameters showed a positive or negative response to waterlogging stress, and this response continuously increased over the treatment time among the genotypes. The CF parameter Fv/Fm was used to screen the 57 accessions, and the results showed clear differences in Fv/Fm between the susceptible and resistant genotypes. Susceptible genotypes had an especially low Fv/Fm value of less than 0.8, reflecting damage to the reaction center of photosystem II. It is concluded that Fv/Fm can be used as a CF parameter index for screening waterlogging stress tolerance in ginseng genotypes.

주제어

표/그림 (7)

표 Table 1 Information on the 57 Korean ginseng genotypes screened for waterlogging tolerance
그림 Fig. 1 Description of scoring criteria for plant phenotypes after abiotic stress treatment. Stressed phenotypes are described separately for each plant part
표 Table 2 Evaluation of tolerance degree after flooding treatment for the 57 Korean ginseng genotypes
그림 Fig. 2 Comparison of the growth of Korean ginseng genotypes at 5, 15 and 25 days of waterlogging treatment
그림 Fig. 3 Comparison of the growth of Korean ginseng genotypes after 25 days of waterlogging treatment
표 Table 3 Effect of waterlogging on root growth traits and SPAD values of Korean ginseng
그림 Fig. 4 Changes in the chlorophyll fluorescence (CF) parameters of Geumjin and GS98-1-5 as representative genotypes over the 25-day duration of waterlogging stress. Use of the same letter ("a" and/or "b") above the point indicates that the results are not significantly different at α = 0.05 according to a Duncan's multiple range test. Vertical bars represent the standard deviation (n = 3)

참고문헌 (31)

Baker NR (2008) Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annual Review Plant Bio 59:89-113
Barraclough PB, Kyte J (2001) Effect of water stress on chlorophyll meter readings in winter wheat. In: Plant Nutrition. Springer, pp.722-723
Bennett JM, Albrecht SL (1984) Drought and flooding effects on N2 fixation, water relations and diffusive resistance of soybean. Agron J 76:735-740
Boyer JS (1970) Differing sensitivity of photosynthesis to low leaf water potentials in corn and soybean. Plant Physiol 46:234-239
Cen H, Weng H, Yao J, He M, Lv J, Hua S, Li H, He Y (2017) Chlorophyll fluorescence imaging uncovers photosynthetic fingerprint of citrus huanglongbing. Front. Plant Sci 8:1509
Choi YM (2018) Exocarp characteristics and the effects of soil water status and fruit set level on growth and fruit quality in a nes cultivar 'Jinok' grape (Vitis labruscana). Doctoral Dissertation. Jeonbuk National University
Filipovic A, Poljak M, Skobic D (2013) Response of chlorophyll a, SPAD values and chlorophyll fluorescence parameters in leaves of apricot affected some abiotic factors. J Food Sci and & Eng 3(1):19-24
Fujii R, N Yamano, H Hashimoto, N Misawa, K Ifuku (2016) Photoprotection vs. photoinhibition of photosystem II in transplastomic lettuce (Lactuca sativa) dominantly accumulating astaxanthin. Plant and Cell Physiology 57:1518-1529

상세보기
Hur SO, Lee YJ, Yeon BR, Jeon SH, Ha SG, Kim JG (2009) Assessment on Water Movement in Paddy-Upland Rotation Soil Scheduled for Ginseng Cultivation. Korean Journal of Medicinal Crop Science 17(3):204-209
Jo JS, Kim CS, Won JY (1996) Crop Rotation of the Korean ginseng (Panax ginseng C.A.Meyer) and the Rice in Paddy Field. Korean Journal of Medicinal Crop Science 4(1):19-26
Kim JU, Lee JW, Jo IH, Kim DH, Kim KH, Kim YC (2015) Growth characteristics of 2-year-old cultivars in Korean ginseng (Panax ginseng C. A. Meyer) conditioned artificial wet injury. CNU Journal of agricultural science 42(4):299-304
Kim KS, Seo YJ, Kim DC, Nam HH, Lee BY, Kim JH (2020) Effect of Soil Water and Shading Treatment on Chlorophyll Fluorescence Parameters and Photosynthetic Capacity in Cnidium officinale Makino. Korean Journal of Medicinal Crop Science 28(6):412-420
Kozlowski TT (1997) Response of woody plants to flooding and salinity. Tree physiology 17(7):490-490
Lee JE, Kim HS, Kwon YU, Jung GH, Lee CK, Yun HT, Kim CK (2010a) Responses of Photosynthetic Characters to Waterlogging in Soybean [Glycine max (L.) Merrill]. Korean Journal of Crop science 55(2):111-118
Lee JE, Kim HS, Kwon YU, Jung GH, Lee CK, Yun HT, Kim CK (2010b) Responses of root growth characters to waterlogging in soybean [Glycine max (L.) Merril]. Korean Journal of Crop science 55(1):1-7
Lee SW, Park KC, Lee SH, Park JM, Jang IB, Kim KH (2013) Soil chemical property and leaf mineral nutrient of ginseng cultivated in paddy field occurring leaf discoloration. Korean Journal of Medicinal Crop Science 21(4):289-295
MAFRA (Ministry of Agriculture, Food and Rural Affairs) (2021) Statistical Sourcebook of Ginseng 2020. pp.1-72. Ministry of Agriculture, Food and Rural Affairs, Sejong, Korea
Maxwell K, Johnson GN (2000) Chlorophyll fluorescence-a practical guide. Journal of experimental botany 51(345): 659-668.

상세보기
Oh DJ, Lee CY, Kim SM, Li GY, Lee SJ, Hwang DY, Son HJ, Won JY (2010) Effects of Chlorophyll Fluorescence and Photosynthesis Characteristics by Planting Positions and Growth Stage in Panax ginseng C. A. Meyer. Korean Journal of Medicinal Crop Science 18(2):65-69
Oh SJ, Zhin KL, Koh SC (2009) Characterization of Chlorophyll a Fluorescence of Hydrophytes under Cadmium Stress. Journal of the Environmental Sciences 18(12):1361-1368
Park EJ, Sohn YG, Park JC, Lee JJ (2006) Effects of NaCl on the growth and inorganic ion contents of green pepper 'Nokwang' and bell pepper 'Newace'. Korean Journal of Horticultural Science Tech 24(1):1-7
Percival GC, Keary IP, Noviss K (2008) The potential of a chlorophyll content SPAD meter to quantify nutrient stress in foliar tissue of sycamore (Acer pseudoplatanus), English oak (Quercus robur), and european beech (Fagus sylvatica). Arboric Urban For 34(2):89-100
Pettigrew WT (2004) Physiological consequences of moisture deficit stress in cotton. Crop Science 44(4):1265-1272

상세보기
Ranjbarfordoei A, Samson R, Damme PV (2006) Chlorophyll fluorescence performance of sweet almond [Prunus dulcis (Miller)] D. Webb] in response to salinity stress induced by NaCl. Photosynthetica 44:513-522

상세보기
RDA (Rural Development Administration) (2001) Revision notice on the standard of Ginseng cultivation method (Notice 2001-8). Suwon, Korea
Shin YK, Jo JS, Cho MC, Yang EY, Ahn YK, Hwang ID, Lee JG (2021) Application of Chlorophyll Fluorescence Parameters to Diagnose Salinity Tolerance in the Seedling of Tomato Genetic Resources. Journal of Bio-Environment Control 30(2):165-173
Snider JL, Collins GD, Whitaker J, Perry CD, Chastain DR (2014) Electron transport throught photosystem II is not limited by a wide range of water-deficit conditions in field grown Gossypium hirsutum. Journal Agronomy and Crop Science 200(1):77-82
Strauss AJ, Kruger GHJ, Strasser RJ, Heerden PDRV (2006) Ranking of dark chilling tolerance in soybean genotypes probed by the chlorophyll a fluorescence transient O-J-I-P. Environmental and experimental botany 56(2):147-157

상세보기
Streb P, Aubert S, Gout E, Feierabend J, Bligny R (2008) Cross tolerance to heavy-metal and cold-induced photoinhibiton in leaves of Pisum sativum acclimated to low temperature. Physiology and molecular biology of plants 14(3):185-193

상세보기
Vu NT, Choi KY, Kim IS (2014) Effect of water content in the substrate of the scion and rootstock during pre-and post-grafting period on the survival rate and quality of tomato plug seedlings. Protected Hort Plant Fac 23(3):199-204
Yang EY (2017) Studies on Physiological and Genetic Characteristics and Breeding of Waterlogging Tolerancein Pepper (Capsicum annuum L.). Doctoral Dissertation, University of Seoul

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증