[논문]애엽(艾葉) 추출물이 알코올성 위염에 미치는 효과

이진아; 서정복; 김진영; 신미래; 박해진; 노성수

doi:10.22246/jikm.2022.43.4.738

애엽(艾葉) 추출물이 알코올성 위염에 미치는 효과
Effect of Artemisiae Argyi Folium Extract on Alcohol-Induced Gastritis 원문보기

대한한방내과학회지 = The journal of internal Korean medicine, v.43 no.4, 2022년, pp.738 - 750

이진아 (대구한의대학교 한의과대학 본초약리학교실) , 서정복 (대구한의대학교 한의과대학 본초약리학교실) , 김진영 (대구한의대학교 한의과대학 본초약리학교실) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 박해진 (DHU 바이오융복합시험센터) , 노성수 (대구한의대학교 한의과대학 본초약리학교실)

Abstract ▼ AI-Helper

Objective: Alcohol is known to cause inflammation in the stomach by decreasing the protective substances of the gastric mucosa and increasing oxidative stress. The purpose of this study was to investigate the anti-inflammatory effect of Artemisiae Argyi Folium extract (AF) on alcohol-induced gastritis. Methods: The total polyphenol and flavonoid contents of AF were confirmed through an in vitro experiment. Radical scavenging activities were confirmed using DPPH and ABTS assays. In vivo experiments were conducted on mice divided into 5 groups (n=8): a normal group (Nor), an alcohol-induced gastritis group (Con), an alcohol-induced gastritis group administered 10 mg/kg sucralfate (SC), an alcohol-induced gastritis group administered 100 mg/kg AF (AFL), and an alcohol-induced gastritis group administered 200 mg/kg AF(AFH). The serum levels of reactive oxygen species (ROS) were determined, and protein expressions were confirmed in gastric tissues. Results: In alcohol-induced gastritis, AF alleviated damage to the gastric mucosa caused by alcohol. AF also decreased the serum ROS levels. Western blots showed that AF decreased the expression of NADPH oxidase and decreased the expression of the NF-κB pathway associated with inflammation. AF also decreased the expression of adhesion molecules and chemokine proteins, and increased the expression of anti-inflammatory proteins. Conclusions: AF not only reduced oxidative stress in alcohol-induced gastritis, but it also relieved gastric mucosal inflammation by regulating the expression of the NF-κB pathway.

주제어

표/그림 (9)

그림 Fig. 1. Optical changes of gastric mucosa in alcohol-induced gastritis.
표 Table 1. Total Polyphenol and Total Flavonoid Cotents of AF
표 Table 2. Scavenging Activity of AF on DPPH and ABTS Radical
그림 Fig. 3. Expression of NADPH oxidase in gastric tissue. Nor : normal group, Con : alcohol-induced gastritis group, SC : sucralfate 10 mg/kg and alcohol-induced gastritis group, AFL : AF 100 mg/kg and alcohol-induced gastritis group, AFH : AF 200 mg/kg and alcohol-induced gastritis group. AF : Artemisiae Argyi Folium extract. All date are expressed mean±SD (n=8). Significance : ^##p<0.01, ^###p<0.001 vs. Nor group and *p<0.05, **p<0.01 vs. Con group
그림 Fig. 2. Levels of serum ROS in alcohol-induced gastritis. Nor : normal group, Con : alcohol-induced gastritis group, SC : sucralfate 10 mg/kg and alcoholinduced gastritis group, AFL : AF 100 mg/kg and alcohol-induced gastritis group, AFH : AF 200 mg/kg and alcohol-induced gastritis group. AF : Artemisiae Argyi Folium extract. All date are expressed mean±SD (n=8). Significance : ^###p<0.001 vs. Nor group and *p<0.05, **p<0.01, ***p<0.001 vs. Con group
그림 Fig. 4. Expression of NF-κBp65 and p-IκBɑ in gastric tissue. Nor : normal group, Con : alcohol-induced gastritis group, SC : sucralfate 10 mg/kg and alcohol-induced gastritis group, AFL : AF 100 mg/kg and alcohol-induced gastritis group, AFH : AF 200 mg/kg and alcohol-induced gastritis group. AF : Artemisiae Argyi Folium extract. All date are expressed mean±SD (n=8). Significance : ^###p<0.001 vs. Nor group and **p<0.01, ***p<0.001 vs. Con group
그림 Fig. 5. Expression of inflammatory protein in gastric tissue. Nor : normal group, Con : alcohol-induced gastritis group, SC : sucralfate 10 mg/kg and alcohol-induced gastritis group, AFL : AF 100 mg/kg and alcohol-induced gastritis group, AFH : AF 200 mg/kg and alcohol-induced gastritis group. AF : Artemisiae Argyi Folium extract. All date are expressed mean±SD (n=8). Significance : ^##p<0.01, ^###p<0.001 vs. Nor group and *p<0.05, **p<0.01, ***p<0.001 vs. Con group
그림 Fig. 6. Expression of anti-inflammatory protein in gastric tissue. Nor : normal group, Con : alcohol-induced gastritis group, SC : sucralfate 10 mg/kg and alcohol-induced gastritis group, AFL : AF 100 mg/kg and alcohol-induced gastritis group, AFH : AF 200 mg/kg and alcohol-induced gastritis group. AF : Artemisiae Argyi Folium extract. All date are expressed mean±SD (n=8). Significance : ^##p<0.01 vs. Nor group and *p<0.05, **p<0.01 vs. Con group
그림 Fig. 7. Expression of adhesion molecule and chemokine in gastric tissue. Nor : normal group, Con : alcohol-induced gastritis group, SC : sucralfate 10 mg/kg and alcohol-induced gastritis group, AFL : AF 100 mg/kg and alcohol-induced gastritis group, AFH : AF 200 mg/kg and alcohol-induced gastritis group. AF : Artemisiae Argyi Folium extract. All date are expressed mean±SD (n=8). Significance : ^##p<0.01, ^###p<0.001 vs. Nor group and *p<0.05, **p<0.01, ***p<0.001 vs. Con group.

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