[논문]알코올성 위염에 대한 조구등(釣鉤藤)의 항염증 효과

이진아; 이태종; 김진영; 신미래; 박해진; 노성수

doi:10.6116/kjh.2022.37.5.63.

알코올성 위염에 대한 조구등(釣鉤藤)의 항염증 효과
Anti-inflammatory effect of Uncariae Ramulus et Uncus on alcohol-induced gastritis 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.5, 2022년, pp.63 - 74

이진아 (대구한의대학교 한의과대학 본초약리학교실) , 이태종 (대구한의대학교 한의과대학 본초약리학교실) , 김진영 (대구한의대학교 한의과대학 본초약리학교실) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 박해진 (DHU 바이오융복합시험센터) , 노성수 (대구한의대학교 한의과대학 본초약리학교실)

Abstract ▼ AI-Helper

Objective : Gastritis refers to an inflammatory disease of the gastric mucosa. Alcohol is one of the main aggression factors, causing bleeding and inflammation in the gastric mucosa and it is known to not only increase lipid peroxide levels, but also deplete key antioxidant factors. The purpose of this study was to determine the effect of Uncariae Ramulus et Uncus water extract (URW) in alcohol-induced gastritis. Methods : The total polyphenol and flavonoid contents of URW were confirmed through an in vitro experiment. Also, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) activity were confirmed. For in vivo experiments, mice were divided into 4 groups (n=8). Also, 1 hr after oral administration of each drug, 50% ethanol was orally administered to induce gastritis. Results : As a result of in vitro experiments, URW showed excellent antioxidant activity. In alcohol-induced gastritis, URW alleviated the damage to the gastric mucosa caused by alcohol. Also, URW decreased reactive oxygen species (ROS) and malondialdehyde (MDA) levels in serum and gastric tissues, and significantly decreased the expression of NADPH oxidases in gastric tissues. In addition, it significantly modulated the nuclear factor erythroid-derived 2-related factor 2 (Nrf2) and nuclear factor-𝜅B p65 (NF-𝜅B) pathways as well as significantly increased the expression of anti-inflammatory proteins. Conclusions : These results suggest that URW not only reduces oxidative stress through excellent antioxidant activity but also relieves gastric mucosal inflammation as a regulator of Nrf2 and NF-𝜅B pathways.

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표/그림 (11)

그림 Fig. 1. DPPH and ABTS radical scavenging activites of URW. DPPH radical scavenging activity, (A); ABTS radical scavenging activity, (B). AS, L-ascorbic acid; URW, Uncariae Ramulus et Uncus water extract. All values are expressed as mean±SEM of three replications.
표 Table 1. Total Polyphenol and Total Flavonoid Contents of URW
그림 Fig. 3. Optical changes of gastric mucosa in alcohol-induced gastritis mice. A representative gross image; (A), gastric mucosal damage ratio; (B). Normal mice, Nor; alcohol-induced gastritis mice, Con; sucralfate 10 mg/kg body weight/day-treated alcohol-induced gastritis mice, SC10; Uncariae Ramulus et Uncus water extract 100 mg/kg body weight/day-treated alcohol-induced gastritis mice, UR100. All data are expressed means±SD (n=8). Significance: ^###p<0.001 vs. Nor group and *p<0.05, **p<0.01 vs. Con group.
그림 Fig. 2. FRAP activity of URW. AS, L-ascorbic acid; URW, Uncariae Ramulus et Uncus water extract. All values are expressed as mean±SEM of three replications.
그림 Fig. 4. Levels of serum and tissue ROS in alcohol-induced gastritis mice. Normal mice, Nor; alcohol-induced gastritis mice, Con; sucralfate 10 mg/kg body weight/day-treated alcohol-induced gastritis mice, SC10; Uncariae Ramulus et Uncus water extract 100 mg/kg body weight/day-treated alcohol-induced gastritis mice, UR100. All data are expressed means±SD (n=8). Significance: ^###p<0.001 vs. Nor group and *p<0.05, **p<0.01, ***p<0.001 vs. Con group.
그림 Fig. 5. Levels of serum and tissue MDA in alcohol-induced gastritis mice. Normal mice, Nor; alcohol-induced gastritis mice, Con; sucralfate 10 mg/kg body weight/day-treated alcohol-induced gastritis mice, SC10; Uncariae Ramulus et Uncus water extract 100 mg/kg body weight/day-treated alcohol-induced gastritis mice, UR100. All data are expressed means±SD (n=8). Significance: ^###p<0.001 vs. Nor group and ***p<0.001 vs. Con group.
그림 Fig. 6. Expressions of NADPH oxidase in alcohol-induced gastritis mice. Normal mice, Nor; alcohol-induced gastritis mice, Con; sucralfate 10 mg/kg body weight/day-treated alcohol-induced gastritis mice, SC10; Uncariae Ramulus et Uncus water extract 100 mg/kg body weight/day-treated alcohol-induced gastritis mice, UR100. All data are expressed means±SD (n=8). Significance: ^##p<0.01, ^###p<0.001 vs. Nor group and *p<0.05, **p<0.01, ***p<0.001 vs. Con group.
그림 Fig. 7. Expressions of antioxidant-related protein in alcohol-induced gastritis mice. Normal mice, Nor; alcohol-induced gastritis mice, Con; sucralfate 10 mg/kg body weight/day-treated alcohol-induced gastritis mice, SC10; Uncariae Ramulus et Uncus water extract 100 mg/kg body weight/day-treated alcohol-induced gastritis mice, UR100. All data are expressed means±SD (n=8). Significance: ^###p<0.001 vs. Nor group and *p<0.05, **p<0.01, ***p<0.001 vs. Con group.
그림 Fig. 8. Expressions of NF-κBp65 and p-IκBɑ in alcohol-induced gastritis mice. Normal mice, Nor; alcohol-induced gastritis mice, Con; sucralfate 10 mg/kg body weight/day-treated alcohol-induced gastritis mice, SC10; Uncariae Ramulus et Uncus water extract 100 mg/kg body weight/day-treated alcohol-induced gastritis mice, UR100. All data are expressed means±SD (n=8). Significance: ^###p<0.001 vs. Nor group and **p<0.01 vs. Con group.
그림 Fig. 9. Expressions of inflammatory protein in alcohol-induced gastritis mice. Normal mice, Nor; alcohol-induced gastritis mice, Con; sucralfate 10 mg/kg body weight/day-treated alcohol-induced gastritis mice, SC10; Uncariae Ramulus et Uncus water extract 100 mg/kg body weight/day-treated alcohol-induced gastritis mice, UR100. All data are expressed means±SD (n=8). Significance: ^##p<0.01, ^###p<0.001 vs. Nor group and *p<0.05, **p<0.01 vs. Con group.
그림 Fig. 10. Expressions of anti-inflammatory protein in alcohol-induced gastritis mice. Normal mice, Nor; alcohol-induced gastritis mice, Con; sucralfate 10 mg/kg body weight/day-treated alcohol-induced gastritis mice, SC10; Uncariae Ramulus et Uncus water extract 100 mg/kg body weight/day-treated alcohol-induced gastritis mice, UR100. All data are expressed means±SD (n=8). Significance: ^##p<0.01, ^###p<0.001 vs. Nor group and ***p<0.001 vs. Con group.

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