[논문]Taxus chinensis로부터 파클리탁셀 정제를 위한 음압 캐비테이션 아세톤-펜테인 분별침전

민혜수; 김진현

doi:10.9713/kcer.2022.60.4.544

Taxus chinensis로부터 파클리탁셀 정제를 위한 음압 캐비테이션 아세톤-펜테인 분별침전
Negative Pressure Cavitation Acetone-Pentane Fractional Precipitation for the Purification of Paclitaxel from Taxus chinensis 원문보기

Korean chemical engineering research = 화학공학, v.60 no.4, 2022년, pp.544 - 549

민혜수 (공주대학교 화학공학부, 미래지속가능기술연구소) , 김진현 (공주대학교 화학공학부, 미래지속가능기술연구소)

초록
AI-Helper

본 연구에서는 음압 캐비테이션 아세톤-펜테인 분별침전으로 Taxus chinensis로부터 파클리탁셀의 침전 효율을 획기적으로 개선하였다. 음압 -200 mmHg에서 침전할 경우 짧은 조업 시간(5분)에 대부분의 파클리탁셀을 회수(>99.9%) 할 수 있었다. 침전 속도 상수는 대조군 대비 1.512~5.073배(-50~-200 mmHg) 증가하였다. 음압으로 활성화에너지는 -3737~-6536 J/mol 감소하였으며, 이로 인해 침전 속도가 증가하였다. 또한 음압 도입으로 침전물 크기는 5.3배 감소하였으며, 파클리탁셀의 확산 계수는 7.0배 증가하였다.

Abstract ▼ AI-Helper

This study presents the negative pressure cavitation acetone-pentane fractional precipitation to dramatically improve the precipitation efficiency of paclitaxel from Taxus chinensis. When paclitaxel was precipitated under a negative pressure of -200 mmHg, most paclitaxel (>99.9%) could be recovered in a short precipitation time (5 min). The precipitation rate constant increased by 1.512~5.073 times (at -50 mmHg to -200 mmHg) compared to the control. The activation energy decreased by -3,737~-6,536 J/mol due to negative pressure, which increased the precipitation rate. With the introduction of negative pressure, the precipitate size decreased by 5.3 times, and the diffusion coefficient of paclitaxel increased by 7.0 times.

주제어

표/그림 (6)

$Fig. 1. Schematic diagram of (A) conventional fractional precipitation and (B) negative pressure cavitation acetone-pentane fractional precipitation for purification of paclitaxel.$ 그림 Fig. 1. Schematic diagram of (A) conventional fractional precipitation and (B) negative pressure cavitation acetone-pentane fractional precipitation for purification of paclitaxel.
$Fig. 2. Effect of precipitation time on the yield of paclitaxel obtained by conventional acetone-pentane fractional precipitation at 5℃ and atmospheric pressure.$ 그림 Fig. 2. Effect of precipitation time on the yield of paclitaxel obtained by conventional acetone-pentane fractional precipitation at 5℃ and atmospheric pressure.
$Fig. 3. Effect of negative pressures (-50, -100, -150, and -200 mmHg) on the yield of paclitaxel obtained by negative pressure cavitation acetone-pentane fractional precipitation at 5℃.$ 그림 Fig. 3. Effect of negative pressures (-50, -100, -150, and -200 mmHg) on the yield of paclitaxel obtained by negative pressure cavitation acetone-pentane fractional precipitation at 5℃.
$Table 1. Comparison of this study and previous studies for acetone-pentane fractional precipitation of paclitaxel$ 표 Table 1. Comparison of this study and previous studies for acetone-pentane fractional precipitation of paclitaxel
$Table 2. The values of kinetic parameter for the acetone-pentane fractional precipitation of paclitaxel at different negative pressures$ 표 Table 2. The values of kinetic parameter for the acetone-pentane fractional precipitation of paclitaxel at different negative pressures
표 Table 3. Effect of negative pressure cavitation bubbles on mean particle size, viscosity, and diffusivity

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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