[논문]타우린 영양 강화 로티퍼 공급이 방어(Seriola quinqueradiata) 부화 자어의 성장 및 부레 팽창에 미치는 영향

김재훈; 박진우; 조정현

doi:10.5657/kfas.2022.0524

타우린 영양 강화 로티퍼 공급이 방어(Seriola quinqueradiata) 부화 자어의 성장 및 부레 팽창에 미치는 영향
Taurine-enriched Rotifers Improve the Growth and Swim Bladder Inflation of Yellowtail Seriola quinqueradiata Larvae 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.55 no.5, 2022년, pp.524 - 532

김재훈 (국립수산과학원 제주수산연구소) , 박진우 (국립수산과학원 제주수산연구소) , 조정현 (국립수산과학원 제주수산연구소)

Abstract ▼ AI-Helper

We investigated the effects of taurine-enriched rotifers on larval growth and swim bladder inflation of yellowtail Seriola quinqueradiata. Rotifers were enriched with a commercial taurine supplement at two levels (0 and 800 mg/L). The larvae (initial notochord length=3.98±0.24 mm) were fed the enriched rotifers in triplicate from two days post-hatch for five days. The average taurine contents of the taurine non-enriched and enriched rotifers were 0.35±0.01 and 4.77±0.05 mg/g dry matter, respectively. The weight gain and specific growth rate of the fish fed enriched rotifers with the taurine supplement at 800 mg/L significantly improved compared with those of fish fed rotifers without taurine enrichment (P<0.05). The swim bladder inflation rate of larvae fed taurine enriched rotifers significantly (P<0.05). The results of the present study indicate that yellowtail larvae benefit from taurine concentrations compared with those typically reported to feed on non-taurine supplemented rotifers. Furthermore, taurine-enriched food for fish larval effectively improved the growth performance and swim bladder inflation of yellowtail larvae.

주제어

표/그림 (5)

표 Table 1. Free amino acid content of chlorella and rotifers (mg/g, dry-matter)
표 Table 2. Fatty acid composition (% of total fatty acids) of chlorella and rotifers
그림 Fig. 1. Growth performance (A, notochord length; B, body weight; C, weight gain; D, specific growth rate) of yellowtail Seriola quinqueradiata larvae fed at the different taurine-enriched rotifers for 5 days (n=3 tanks; twenty pooled fish per tank). Vertical bar indicates the standard error of mean values of triplicate tanks. The asterisks indicate significant differences between T-0 and T-800 by t-test (P<0.05).
$Fig. 2. The mean of the fractions of larvae with inflated swim bladders of yellowtail Seriola quinqueradiata larvae fed at the different taurine-enriched rotifers for 5 days (n=3 tanks; 30 fish per tank). Vertical bar indicates the standard error of mean values of triplicate tanks. The asterisks indicate significant differences between T-0 and T-800 by t-test (P<0.05).$ 그림 Fig. 2. The mean of the fractions of larvae with inflated swim bladders of yellowtail Seriola quinqueradiata larvae fed at the different taurine-enriched rotifers for 5 days (n=3 tanks; 30 fish per tank). Vertical bar indicates the standard error of mean values of triplicate tanks. The asterisks indicate significant differences between T-0 and T-800 by t-test (P<0.05).
그림 Fig. 3. Image of yellowtail Seriola quinqueradiata larvae showing successful (upper) and unsuccessful (lower) first swim bladder inflation. In (A) the swim bladder can be seen to be inflated via the presence of a bubble just above the gut while this feature is absent in (B).

AI 본문요약
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제안 방법

방어 자어는 어체 성장 분석을 위해 부화 후 0일령에서 7일령까지 매일 수조 당 30마리씩 무작위로 채취하여 척삭 길이(notochord length)와 어체중(weight)을 측정하고 척추 아래 부분 형성되는 부레 발달 유무를 확인하였다. 방어 자어의 척삭 길이 측정과 부레 발달 유무 확인은 stereoscopic microscope (SMZ745T; Nikon, Tokyo, Japan)과 image software (Optiview; Korea Lab Tech, Seongnam, Korea)을 이용하였고, 어체중은 미세 저울(Quintix124-1SKR; Sartorius, Goettingen, Germany)로 0.
방어 자어의 척삭 길이 측정과 부레 발달 유무 확인은 stereoscopic microscope (SMZ745T; Nikon, Tokyo, Japan)과 image software (Optiview; Korea Lab Tech, Seongnam, Korea)을 이용하였고, 어체중은 미세 저울(Quintix124-1SKR; Sartorius, Goettingen, Germany)로 0.1 μg까지 측정하였다
사육 실험 수조 내 로티퍼의 공급은 영양 강화 수조에서 12시간 및 18시간 강화한 로티퍼를 채취하여 25°C 해수에 5분 이상 세척한 후 일 2회(10:00 및 16:00) 공급하였다
1 μg까지 측정하였다. 증체율(weight gain, WG), 일간 성장률(specific growth rate, SGR) 및 부레 팽창률(swim bladder inflation rate, SBIR)을 각각 아래와 같이 계산하였다.

대상 데이터

국립수산과학원 제주수산연구소(Jeju, Korea)에서 사육 관리 중인 방어 어미(5세어, 어체중 약 6–7 kg)로부터 자연 산란된(5월) 수정란을 얻어 폴리에틸렌 재질의 150 L 실내 원형 수조 6개에 수조당 3,000립씩 3반복구로 수용하여 부화시켰다(부화율 76.2±11.0%, n=3)
0%, n=3). 실험은 방어 부화 자어의 개구기(mouthopening stage)가 진행된 2일령부터 수행하였다.

데이터처리

데이터의 통계적 분석은 95%의 유의 수준(P<0.05)으로 ANOVA를 사용하여 수행하였다
05)으로 ANOVA를 사용하여 수행하였다. 유의한 차이(significant treatment effect)가 감지되면 t-검정을 사용하여 평균간의 유의적인 차이를 평가하였다.

성능/효과

chlorella의 DHA는 검출되지 않았다. DHA 영양 강화를 하지 않은 초기 로티퍼의 지방산 내 DHA 함량은 0.27%를 나타냈으나, T-0 및 T-800의 DHA 함량은 각각 5.35% 및 5.43%로 DHA 영양 강화 효과가 확인되었다(Table 2).
부레 팽창률은 T-0구의 경우 58.7%였으며, T-800구의 경우 84.4%로 T-0구와 비교하여 유의적으로 높았다(P<0.05).

후속연구

본 연구는 로티퍼의 타우린 영양강화가 자어기 방어의 성장 및 부레 팽창율 개선에 효과적임을 시사하며, 상기 결과는 방어를 포함한 해산어의 종자 생산 시 로티퍼의 타우린 영양강화의 필요성을 더욱 이해하는데 기초자료로 활용이 가능할 것으로 판단된다. 그러나 종자생산 시 효과적인 타우린 영양 강화를 위해서는 최적 영양 강화 농도 및 강화 시간 등을 구명하는 연구들이 수반되어야 할 것이다.
본 연구는 로티퍼의 타우린 영양강화가 자어기 방어의 성장 및 부레 팽창율 개선에 효과적임을 시사하며, 상기 결과는 방어를 포함한 해산어의 종자 생산 시 로티퍼의 타우린 영양강화의 필요성을 더욱 이해하는데 기초자료로 활용이 가능할 것으로 판단된다. 그러나 종자생산 시 효과적인 타우린 영양 강화를 위해서는 최적 영양 강화 농도 및 강화 시간 등을 구명하는 연구들이 수반되어야 할 것이다.

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Yokoyama M, Takeuchi T, Park GS and Nakazoe J. 2001. Hepatic cysteinesulphinate decarboxylase activity in fish. Aquac Res 32, 216-220. https://doi.org/10.1046/j.1355-557x.2001.00017.x.

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Yoshimatsu T and Hossain MA. 2014. Recent advances in the high-density rotifer culture in Japan. Aquac Int 22, 1587-1603. https://doi.org/10.1007/s10499-014-9767-5.

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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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