[논문]사육 밀도가 무지개송어(Oncorhynchus mykiss) 치어의 성장, 체조성, 스트레스, 항산화 및 면역반응에 미치는 영향

김재훈; 황인준; 박진우; 조정현

doi:10.5657/kfas.2023.0079

사육 밀도가 무지개송어(Oncorhynchus mykiss) 치어의 성장, 체조성, 스트레스, 항산화 및 면역반응에 미치는 영향
Effects of Stocking Density on the Growth, Body Composition, Stress, Antioxidant Status and Immune Responses of Juvenile Rainbow Trout Oncorhynchus mykiss 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.56 no.1, 2023년, pp.79 - 88

김재훈 (국립수산과학원 제주수산연구소) , 황인준 (국립수산과학원 서해수산연구소) , 박진우 (국립수산과학원 제주수산연구소) , 조정현 (국립수산과학원 제주수산연구소)

Abstract ▼ AI-Helper

A 12-week feeding trial was conducted to evaluate the effect of different stocking densities on the growth, body composition, stress, antioxidant status, and immune responses of juvenile rainbow trout Oncorhynchus mykiss. Juvenile rainbow trout (32.2±0.5 g) were randomly placed in eight experimental tanks, each containing 2,000 L of water, at stocking densities of 150, 300, 600, and 1,200 individual/m3. The trout were assigned to four treatments (2.4, 4.8, 9.7, and 19.4 kg/m3) in duplicates. Following after the feeding trial, the fish cultured in the less dense group showed significantly higher growth and survival (P<0.05). In terms of proximate composition, the whole body crude protein and lipid contents were lower in the denser group (19.4 kg/m3). Regarding the antioxidant and immune responses, the fish reared in the relatively denser group exhibited lower lysozyme, immunoglobulin M, and superoxide dismutase levels. The stress indicators, glucose and cortisol, were significantly lower in the less dense group (2.4 kg/m3), while the denser group (19.4 km/m3) exhibited higher AST and ALP levels(P<0.05). The results showed that denser stocking has a crowding stress effect and negatively impacts growth and immune responses.

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표/그림 (8)

표 Table 1. Rearing conditions for feeding trial of juvenile rainbow trout Oncorhynchus mykiss
그림 Fig. 1. Survival of juvenile rainbow trout Oncorhynchus mykiss at different initial stocking densities for 12 weeks.
그림 Fig. 2. Pearson correlation coefficients between initial stocking density and final body weight (left) and correlation coefficients between final stocking density and final body weight (right). ^**P<0.01.
표 Table 2. Growth performance and biological indices of juvenile rainbow trout Oncorhynchus mykiss at different initial stocking densities for 12 weeks¹
그림 Fig. 3. A two-slope straight (solid line) and quadratic (dashed line) broken-line regression models were fitted to datasets of final biomass in response to the initial stocking density of the juvenile rainbow trout Oncorhynchus mykiss. The equation of broken-line models for the final biomass dataset are [final biomass (kg/m³)=13.3608+0.9927(x-9.0040)+0.2722(x-9.0040) (R²= 0.9557), where (x-9.0040) is defined as zero at the values of (x<9.0040) and [final biomass (kg/m³)=15.0520-0.0498x(x- 11.2138)+0.5373(x-11.2138) (R²=0.9612), where (x-11.2138) is defined as zero at the values of (x<11.2138). The x represents initial stocking density (kg/m³).
표 Table 3. Proximate composition of whole-body and dorsal muscle of rainbow trout Oncorhynchus mykiss at different initial stocking densities for 12 weeks¹
표 Table 4. Plasma metabolites of rainbow trout Oncorhynchus mykiss at different initial stocking densities for 12 weeks¹
그림 Fig. 4. Interaction effect means of plasma lysozyme, immunoglobulin M, superoxide dismutase and cortisol of rainbow trout Oncorhynchus mykiss at different initial stocking densities for 12 weeks. Statistical difference in the concentration (mean±SEM) within the interaction effect (N=2 tanks; thirty pooled fish per tank) in response to the different feeding rates was analyzed by one-way analysis of variance (ANOVA). When significance (P<0.05) was detected, multiple range test using the Tukey's honestly significant difference was performed.

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제안 방법

사육 실험 종료 시에는 24시간 절식 후 각 실험구별 전체 무게를 측정하여 증체율(weight gain, WG), 일간 성장률(specific growth rate, SGR), 사료 계수(feed conversion ratio, FCR) 및 생존율(survival)을 계산하였다. 생물학적 지표를 조사하기 위해 각 수조별 30마리를 무작위로 취하여 비만도(condition factor, CF), 간 중량 지수(hepatosomatic index, HSI), 내장 중량 지수(viscerosomatic index, VSI) 및 내장지방 지수(intraperitoneal fat index, IFI)를 계산하였다.
사육 실험 종료 시에는 24시간 절식 후 각 실험구별 전체 무게를 측정하여 증체율(weight gain, WG), 일간 성장률(specific growth rate, SGR), 사료 계수(feed conversion ratio, FCR) 및 생존율(survival)을 계산하였다. 생물학적 지표를 조사하기 위해 각 수조별 30마리를 무작위로 취하여 비만도(condition factor, CF), 간 중량 지수(hepatosomatic index, HSI), 내장 중량 지수(viscerosomatic index, VSI) 및 내장지방 지수(intraperitoneal fat index, IFI)를 계산하였다. 각각의 지표는 아래와 같이 계산하였다.

대상 데이터

스트레스, 항산화 및 면역 분석은 혈액 성상 분석 조사에 사용된 30마리의 혈액을 이용하여 수행하였다. 혈중 lysozyme (Lyz), immunoglobulin M (IgM), superoxide dismutase (SOD) 활성 및 cortisol 측정은 상업용 측정 키트를 사용하여 (Cat.

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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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